Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACKWD1_RS15705 Genome accession   NZ_CP177201
Coordinates   3354048..3355487 (-) Length   479 a.a.
NCBI ID   WP_039040474.1    Uniprot ID   -
Organism   Aeromonas caviae strain QZ63     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3349048..3360487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWD1_RS15675 (ACKWD1_15675) dagF 3349326..3350066 (+) 741 WP_201921325.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  ACKWD1_RS15680 (ACKWD1_15680) - 3350171..3352075 (+) 1905 WP_201985175.1 BglG family transcription antiterminator -
  ACKWD1_RS15685 (ACKWD1_15685) npr 3352091..3352363 (-) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  ACKWD1_RS15690 (ACKWD1_15690) rapZ 3352384..3353250 (-) 867 WP_201985173.1 RNase adapter RapZ -
  ACKWD1_RS15695 (ACKWD1_15695) ptsN 3353291..3353737 (-) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACKWD1_RS15700 (ACKWD1_15700) hpf 3353740..3354027 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  ACKWD1_RS15705 (ACKWD1_15705) rpoN/rpoN1 3354048..3355487 (-) 1440 WP_039040474.1 RNA polymerase factor sigma-54 Machinery gene
  ACKWD1_RS15710 (ACKWD1_15710) lptB 3355553..3356278 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  ACKWD1_RS15715 (ACKWD1_15715) lptA 3356291..3356809 (-) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  ACKWD1_RS15720 (ACKWD1_15720) lptC 3356796..3357356 (-) 561 WP_039040473.1 LPS export ABC transporter periplasmic protein LptC -
  ACKWD1_RS15725 (ACKWD1_15725) kdsC 3357353..3357907 (-) 555 WP_042864828.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACKWD1_RS15730 (ACKWD1_15730) - 3357907..3358902 (-) 996 WP_042880205.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACKWD1_RS15735 (ACKWD1_15735) mlaF 3359109..3359909 (+) 801 WP_029314846.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53761.57 Da        Isoelectric Point: 4.3728

>NTDB_id=1086142 ACKWD1_RS15705 WP_039040474.1 3354048..3355487(-) (rpoN/rpoN1) [Aeromonas caviae strain QZ63]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQTETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=1086142 ACKWD1_RS15705 WP_039040474.1 3354048..3355487(-) (rpoN/rpoN1) [Aeromonas caviae strain QZ63]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGACAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACGGCCCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAGGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCTGCCATCGCCCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCGGACCTGCCCT
GGCTCGACGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTTGAACTCAATCCGG
ATTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCTCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAAGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCGAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482

Multiple sequence alignment