Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   ACLB6K_RS15215 Genome accession   NZ_CP177167
Coordinates   3120501..3123125 (-) Length   874 a.a.
NCBI ID   WP_069475043.1    Uniprot ID   -
Organism   Microcystis aeruginosa FACHB-524     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3115501..3128125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB6K_RS15205 (ACLB6K_15200) - 3117309..3118646 (-) 1338 WP_123231115.1 AAA-like domain-containing protein -
  ACLB6K_RS15210 (ACLB6K_15205) - 3119213..3120424 (-) 1212 WP_002738839.1 glycosyltransferase -
  ACLB6K_RS15215 (ACLB6K_15210) pilJ 3120501..3123125 (-) 2625 WP_069475043.1 methyl-accepting chemotaxis protein Machinery gene
  ACLB6K_RS15220 (ACLB6K_15215) - 3123219..3123749 (-) 531 WP_002751697.1 chemotaxis protein CheW -
  ACLB6K_RS15225 (ACLB6K_15220) hmpF 3125339..3126964 (+) 1626 WP_002739140.1 pilus motility taxis protein HmpF -
  ACLB6K_RS15230 (ACLB6K_15225) - 3127363..3127587 (+) 225 WP_002738436.1 hypothetical protein -

Sequence

Protein


Download         Length: 874 a.a.        Molecular weight: 95003.83 Da        Isoelectric Point: 4.3318

>NTDB_id=1085761 ACLB6K_RS15215 WP_069475043.1 3120501..3123125(-) (pilJ) [Microcystis aeruginosa FACHB-524]
MPSGKEYAKLYGKANTAYCQGNLEDAAVIVKEMISKYPDDANVILLQGHIYVGLQQYSLAEGRYEKVLELAKNSANFFEL
VDYARRGLDQIQQSRFEAENGDFMIAAAESIAYSEAAFDPSQGFSNSLSGRSQAADRDQQQEVMDWDSGLFNDEDFGEPT
AGTVDSYGDDFGESEETAFSSLILAGSGSHGSQWSGDSADGEPPFPFLEAAEIDPLQEGWGGGLGSTGEATFTADWRENP
VTGRDQELSYADAPETSTFAIEPELPRRQAAKTDLNQGRVTQKRYEEDEARDFRELKLNDEDLDGFSQLRLTDIGEELGE
SELFTGSGEEALTAGFVPEPRASLNASSAVGEPSFHPSRLEIPDDKRDSHLGPVSERLLGPVVEVNPGKLAFFVNASLGK
KQLILAALAGITPVVLIFAVSTTSWLSAKVAPKPAPIPPAPASLSPWSQPKPAMMLLTGLGTFALTWLGLQLLISQIKRS
LDDLQSQFDHLSEGNFNAKATIYSEDEFGQLANRFNQMAKVVATITTLAQGRAAETERERKNLQRQVIRLLDDVEGAARG
DLTVEAEVSADVLGAVADAFNLTIQNLREIVRQVKKAAKQVNQASTNSESFARNQSSDALRMAEELAVTLNSVQMMTDSI
QRVAENAREAEEVARTSSITALKGGDSVERTVAGILQIRDTVSETARKVKRLAEASQEISKIVAVVSQIASRTNLLALNA
SIQAARAGDAGRGFAVVADEVRQLAYRSAKSLKEIEQIVLQIQSETGSVMMAMEEGIQQVIDVTERSEQAKKSLEDIIQV
SNRIDTLVRSITADTVKQRENSLNVAQVMQSVELTAQETSQESQQVAGSLQKLVSISRELLSSVERFKIDSEDN

Nucleotide


Download         Length: 2625 bp        

>NTDB_id=1085761 ACLB6K_RS15215 WP_069475043.1 3120501..3123125(-) (pilJ) [Microcystis aeruginosa FACHB-524]
ATGCCGTCAGGGAAAGAATATGCGAAACTCTATGGCAAGGCGAACACCGCCTATTGCCAGGGCAACCTTGAGGATGCCGC
CGTCATTGTCAAAGAAATGATCAGCAAGTATCCCGATGATGCCAACGTCATCCTTTTACAGGGACACATTTATGTCGGAC
TACAACAATACAGCTTGGCCGAGGGACGTTACGAAAAAGTGCTGGAATTGGCCAAAAATTCTGCCAATTTCTTTGAGCTG
GTAGATTATGCTCGACGGGGTCTCGATCAGATTCAGCAATCGCGTTTTGAAGCCGAGAATGGAGATTTTATGATCGCCGC
TGCGGAAAGCATTGCCTACAGCGAGGCAGCTTTCGATCCCAGTCAAGGTTTCAGCAATTCTTTGTCTGGGCGATCGCAAG
CTGCTGATCGAGATCAGCAACAGGAGGTAATGGACTGGGATAGTGGTCTTTTCAATGACGAGGATTTCGGAGAGCCTACG
GCGGGTACAGTTGATTCCTATGGCGATGATTTTGGGGAATCCGAGGAAACCGCCTTTAGTTCCCTTATCCTGGCTGGTTC
AGGCTCTCACGGTTCCCAGTGGTCCGGGGATTCTGCGGATGGAGAACCCCCTTTCCCCTTTTTGGAAGCAGCGGAGATTG
ACCCGCTGCAGGAAGGGTGGGGCGGTGGGTTAGGATCAACGGGAGAAGCCACTTTTACGGCTGACTGGCGGGAAAATCCC
GTCACCGGTCGAGATCAGGAGTTATCCTATGCCGATGCCCCAGAAACAAGCACTTTTGCCATTGAACCGGAACTGCCCCG
CCGACAAGCGGCGAAAACTGACCTTAATCAGGGCAGAGTGACTCAAAAAAGATACGAGGAAGACGAAGCCAGAGATTTTA
GAGAACTAAAACTCAATGATGAGGATTTAGACGGTTTTTCCCAACTACGTCTGACGGATATAGGCGAAGAACTGGGGGAA
TCGGAGCTATTTACCGGTTCGGGAGAGGAAGCTTTAACTGCTGGTTTTGTCCCCGAACCCAGGGCGAGCCTTAATGCCAG
TTCTGCTGTGGGAGAGCCTAGTTTTCATCCTTCTCGTCTAGAAATCCCCGATGACAAAAGAGATAGTCATCTTGGCCCTG
TTTCCGAGCGCCTGCTCGGGCCAGTGGTGGAAGTCAATCCGGGCAAATTAGCCTTTTTTGTCAACGCTTCTTTGGGCAAA
AAACAACTAATTTTAGCGGCTTTAGCGGGTATTACCCCAGTGGTGTTAATTTTTGCCGTTAGCACCACCTCTTGGCTTTC
TGCAAAAGTTGCCCCCAAACCAGCCCCAATACCACCAGCACCAGCCAGCCTTTCTCCTTGGAGTCAACCGAAACCAGCAA
TGATGTTATTAACTGGATTGGGAACCTTTGCCCTAACTTGGCTAGGTTTACAGTTGCTAATTAGTCAAATTAAGCGCAGT
CTCGACGATTTGCAAAGCCAATTCGATCATCTTTCGGAAGGCAATTTTAACGCTAAAGCGACGATCTACTCGGAAGATGA
ATTTGGTCAACTGGCCAATCGTTTCAATCAGATGGCCAAGGTGGTGGCAACTATCACCACCTTGGCCCAAGGCCGCGCCG
CCGAAACGGAACGGGAACGGAAAAATCTACAAAGGCAAGTGATTCGACTTCTCGATGATGTGGAAGGAGCGGCTCGGGGG
GATCTGACCGTGGAAGCGGAGGTAAGTGCCGATGTTTTAGGGGCGGTGGCCGATGCTTTTAATTTGACGATCCAAAACCT
GCGCGAAATTGTCCGACAGGTGAAAAAAGCGGCAAAACAGGTGAATCAAGCCAGTACCAATAGTGAATCTTTCGCCCGCA
ATCAGTCTAGCGATGCCCTGCGGATGGCGGAGGAGTTGGCCGTCACCCTTAATTCGGTACAGATGATGACCGACTCAATT
CAAAGGGTGGCAGAAAACGCCAGGGAAGCCGAAGAAGTCGCCCGCACTTCCTCGATTACCGCTCTCAAGGGGGGCGATTC
CGTGGAAAGAACCGTGGCGGGAATTCTGCAAATCCGCGACACGGTATCAGAAACGGCGCGAAAAGTCAAGCGTTTGGCAG
AAGCTTCCCAGGAAATCTCGAAAATTGTCGCAGTAGTCTCTCAGATTGCCTCGCGAACCAATTTATTGGCTCTTAATGCC
TCGATTCAAGCGGCGCGAGCAGGAGATGCAGGCCGCGGTTTTGCGGTGGTGGCCGATGAGGTACGTCAGTTAGCCTATAG
ATCGGCTAAATCTCTTAAGGAAATTGAACAGATCGTTTTACAGATTCAATCGGAAACGGGTTCGGTAATGATGGCAATGG
AGGAAGGGATTCAACAGGTTATCGATGTCACCGAGCGCTCGGAACAGGCCAAGAAGTCCCTAGAAGATATTATCCAAGTT
TCTAACCGCATCGATACGCTGGTGCGATCGATTACCGCCGATACGGTCAAACAACGGGAAAATTCCCTCAATGTCGCCCA
AGTTATGCAGTCGGTGGAGTTAACCGCTCAGGAAACTTCCCAAGAATCTCAACAGGTGGCGGGTTCTTTGCAAAAATTGG
TGAGTATCTCCCGGGAGCTGCTTTCTTCGGTAGAACGGTTTAAGATAGACTCGGAAGATAATTAA

Domains

Predicted by InterproScan.

HAMP domain (HAMP)

(472-520)

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(652-832)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

49.731

100

0.529

Multiple sequence alignment