Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACLHWC_RS02565 Genome accession   NZ_CP177122
Coordinates   523946..525316 (+) Length   456 a.a.
NCBI ID   WP_193989204.1    Uniprot ID   -
Organism   Vagococcus salmoninarum strain 2022-116     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 518946..530316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLHWC_RS02540 (ACLHWC_02540) - 519594..519899 (+) 306 WP_126779326.1 rhodanese-like domain-containing protein -
  ACLHWC_RS02545 (ACLHWC_02545) - 519924..521579 (+) 1656 WP_193989201.1 FAD-dependent oxidoreductase -
  ACLHWC_RS02550 (ACLHWC_02550) - 521588..521845 (+) 258 WP_193989202.1 metal-sensitive transcriptional regulator -
  ACLHWC_RS02555 (ACLHWC_02555) - 521952..523067 (+) 1116 WP_193989203.1 VanZ family protein -
  ACLHWC_RS02560 (ACLHWC_02560) - 523139..523669 (+) 531 WP_126779333.1 dUTP diphosphatase -
  ACLHWC_RS02565 (ACLHWC_02565) radA 523946..525316 (+) 1371 WP_193989204.1 DNA repair protein RadA Machinery gene
  ACLHWC_RS02570 (ACLHWC_02570) - 525389..526522 (+) 1134 WP_170174731.1 PIN/TRAM domain-containing protein -
  ACLHWC_RS02575 (ACLHWC_02575) ispD 526543..527247 (+) 705 WP_193989205.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACLHWC_RS02580 (ACLHWC_02580) ispF 527279..527755 (+) 477 WP_193989206.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACLHWC_RS02585 (ACLHWC_02585) gltX 527771..529234 (+) 1464 WP_193989207.1 glutamate--tRNA ligase -
  ACLHWC_RS02590 (ACLHWC_02590) - 529509..529955 (+) 447 WP_126779344.1 DUF3290 domain-containing protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49872.41 Da        Isoelectric Point: 8.0312

>NTDB_id=1085430 ACLHWC_RS02565 WP_193989204.1 523946..525316(+) (radA) [Vagococcus salmoninarum strain 2022-116]
MAKKAKTQFECQACGYISPKYLGRCPNCGAWNEMLEVKIQEEASRNSRVSMAGRRTTATKLGDVTSRKEPRVKTKLDELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLHQSGGKVLYVSGEESAEQIKMRAERLSIEGNDFFVYPETDMSAIRQ
TIQELEPDYVIIDSIQTMMQPEISSATGSVSQVRETTAELMQIAKTNGIAIFIVGHVTKEGSLAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMKEKGLVEVNNPSEVFLEERIDGATGSAIVVSMEGTRPILVEVQSLVTPSLFG
NAKRTSTGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYKENGTKPDECFIGEIGLTG
EIRRVSRIEQRVGEAKKLGFKKIYVPKNNLHGWEPPTGIKIVGVATIGEALRKIFT

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1085430 ACLHWC_RS02565 WP_193989204.1 523946..525316(+) (radA) [Vagococcus salmoninarum strain 2022-116]
GTGGCAAAAAAAGCAAAAACTCAGTTTGAATGTCAAGCTTGTGGCTATATCTCACCTAAATATTTAGGCCGTTGTCCAAA
CTGTGGGGCTTGGAATGAGATGTTAGAAGTTAAGATTCAAGAAGAAGCTTCTCGTAACAGTCGAGTTAGTATGGCTGGTC
GTCGAACGACAGCGACGAAACTCGGAGATGTTACTTCACGAAAAGAACCTAGAGTCAAAACGAAATTAGACGAATTAAAC
CGAGTATTAGGTGGCGGAGTAGTGCCAGGTTCTCTAGTTTTAATTGGTGGTGACCCAGGGATTGGGAAATCGACGTTATT
ATTACAAGTCTCACAACAACTTCATCAAAGTGGCGGTAAAGTGTTATACGTCTCAGGTGAAGAAAGTGCGGAACAAATTA
AGATGCGAGCGGAACGTTTAAGTATTGAAGGCAACGACTTTTTTGTCTATCCAGAAACGGATATGTCAGCTATTCGGCAA
ACGATTCAAGAATTAGAACCAGATTATGTGATTATTGATTCAATTCAAACGATGATGCAGCCGGAAATAAGCAGTGCTAC
AGGTAGTGTTAGCCAAGTTCGCGAAACTACTGCGGAATTAATGCAAATTGCTAAAACCAACGGTATCGCCATCTTTATTG
TTGGTCATGTGACGAAAGAAGGCTCACTGGCAGGACCGCGGATGTTAGAACACATGGTTGATACCGTCCTTTATTTTGAA
GGGGATCGCCATCATACTTTTAGAATTTTACGAGCCGTGAAAAATCGTTTTGGTTCAACAAATGAGATTGGCATTTTTGA
AATGAAAGAAAAAGGCTTAGTTGAAGTGAATAATCCTTCCGAAGTCTTTCTTGAAGAACGAATCGATGGGGCGACTGGTT
CAGCGATTGTTGTTTCAATGGAAGGGACGCGGCCGATTTTAGTTGAAGTTCAATCTTTAGTCACGCCAAGTCTGTTCGGT
AATGCTAAAAGAACATCAACAGGTTTAGACTATAACCGGGTGTCTTTAATTATGGCAGTCCTAGAAAAACGAGCTGGGCT
CTTATTACAAAATCAAGATGCCTATTTAAAAGCCGCAGGTGGTGTGAAATTAGATGAACCAGCCATTGATTTAGCGATAG
CCGTTGCGATAGCTTCGAGTTATAAAGAAAATGGCACGAAACCTGATGAGTGTTTTATCGGTGAAATTGGTTTAACTGGT
GAAATTCGACGGGTCAGTCGGATTGAGCAACGTGTTGGCGAAGCTAAAAAACTAGGATTTAAAAAGATTTATGTTCCGAA
GAATAATCTCCATGGATGGGAGCCGCCAACAGGCATTAAGATTGTTGGAGTTGCCACAATTGGCGAAGCATTACGTAAAA
TTTTCACTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-32)

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

73.187

99.781

0.73
  radA Streptococcus mitis NCTC 12261

73.187

99.781

0.73
  radA Streptococcus pneumoniae Rx1

72.967

99.781

0.728
  radA Streptococcus pneumoniae D39

72.967

99.781

0.728
  radA Streptococcus pneumoniae R6

72.967

99.781

0.728
  radA Streptococcus pneumoniae TIGR4

72.967

99.781

0.728
  radA Bacillus subtilis subsp. subtilis str. 168

67.257

99.123

0.667

Multiple sequence alignment