Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACLIMH_RS25360 Genome accession   NZ_CP177046
Coordinates   4858966..4860315 (-) Length   449 a.a.
NCBI ID   WP_411469780.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain MPB12     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4853966..4865315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLIMH_RS25340 (ACLIMH_25340) secA 4854288..4856795 (-) 2508 WP_000579383.1 preprotein translocase subunit SecA -
  ACLIMH_RS25345 (ACLIMH_25345) hpf 4857072..4857614 (-) 543 WP_000671190.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACLIMH_RS25350 (ACLIMH_25350) cspC 4857938..4858135 (-) 198 WP_001990088.1 cold shock protein CspC -
  ACLIMH_RS25355 (ACLIMH_25355) comFC 4858262..4858966 (-) 705 WP_047957195.1 comF operon protein ComFC -
  ACLIMH_RS25360 (ACLIMH_25360) comFA 4858966..4860315 (-) 1350 WP_411469780.1 ATP-dependent helicase ComFA Machinery gene
  ACLIMH_RS25365 (ACLIMH_25365) - 4860443..4861756 (-) 1314 WP_071728262.1 NlpC/P60 family protein -
  ACLIMH_RS25370 (ACLIMH_25370) - 4862011..4862325 (-) 315 WP_000400857.1 winged helix-turn-helix transcriptional regulator -
  ACLIMH_RS25375 (ACLIMH_25375) - 4862499..4863341 (-) 843 WP_000844766.1 DegV family protein -
  ACLIMH_RS25380 (ACLIMH_25380) - 4863581..4864216 (+) 636 WP_411469781.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51247.91 Da        Isoelectric Point: 9.5047

>NTDB_id=1085283 ACLIMH_RS25360 WP_411469780.1 4858966..4860315(-) (comFA) [Bacillus thuringiensis strain MPB12]
MLAGKQLLLEELPSDLRRELSDLKKEGEVICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRTGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEIGLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVIYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1085283 ACLIMH_RS25360 WP_411469780.1 4858966..4860315(-) (comFA) [Bacillus thuringiensis strain MPB12]
ATGCTCGCTGGAAAGCAGTTACTATTAGAAGAACTCCCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGGAGGG
AGAGGTCATATGTGTACAAGGCGTAATAAAGAAAGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGCTGGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAAAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAACGGGGAAACAAAA
AGGTATCATTGTTTCGGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAGGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTCGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTATTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(108-254)

Helicase conserved C-terminal domain (Helicase_C)

(312-408)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

52.153

93.096

0.486
  comFA Latilactobacillus sakei subsp. sakei 23K

40.704

88.641

0.361

Multiple sequence alignment