Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACLIMH_RS21040 Genome accession   NZ_CP177046
Coordinates   4058368..4059114 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus thuringiensis strain MPB12     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4053368..4064114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLIMH_RS21025 (ACLIMH_21025) dnaG 4054135..4055931 (-) 1797 WP_000528216.1 DNA primase -
  ACLIMH_RS21030 (ACLIMH_21030) - 4056786..4057598 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  ACLIMH_RS21035 (ACLIMH_21035) - 4057628..4058260 (-) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  ACLIMH_RS21040 (ACLIMH_21040) recO 4058368..4059114 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  ACLIMH_RS21045 (ACLIMH_21045) - 4059148..4059291 (-) 144 WP_000883924.1 YqzL family protein -
  ACLIMH_RS21050 (ACLIMH_21050) era 4059468..4060373 (-) 906 WP_001080241.1 GTPase Era -
  ACLIMH_RS21055 (ACLIMH_21055) - 4060366..4060764 (-) 399 WP_087932366.1 cytidine deaminase -
  ACLIMH_RS21060 (ACLIMH_21060) - 4060864..4061217 (-) 354 WP_001989858.1 diacylglycerol kinase family protein -
  ACLIMH_RS21065 (ACLIMH_21065) ybeY 4061214..4061684 (-) 471 WP_000054692.1 rRNA maturation RNase YbeY -
  ACLIMH_RS21070 (ACLIMH_21070) - 4061681..4063825 (-) 2145 WP_000946454.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=1085273 ACLIMH_RS21040 WP_000487010.1 4058368..4059114(-) (recO) [Bacillus thuringiensis strain MPB12]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=1085273 ACLIMH_RS21040 WP_000487010.1 4058368..4059114(-) (recO) [Bacillus thuringiensis strain MPB12]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTTTCAAG
AGAATTGGGTAAGGTAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGTCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGACACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGAGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTACCGTATACCAGTGGG
GGAAGCTGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGGCTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment