Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACLIMH_RS20775 Genome accession   NZ_CP177046
Coordinates   4005615..4006658 (-) Length   347 a.a.
NCBI ID   WP_001013211.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain MPB12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4000615..4011658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLIMH_RS20730 (ACLIMH_20730) - 4001042..4001242 (-) 201 WP_000106081.1 YqzE family protein -
  ACLIMH_RS20735 (ACLIMH_20735) aroK 4001281..4001778 (-) 498 WP_000836631.1 shikimate kinase AroK -
  ACLIMH_RS20740 (ACLIMH_20740) - 4001899..4002549 (-) 651 WP_000183878.1 2OG-Fe(II) oxygenase -
  ACLIMH_RS20745 (ACLIMH_20745) comGG 4002725..4003096 (-) 372 WP_001231192.1 competence type IV pilus minor pilin ComGG -
  ACLIMH_RS20750 (ACLIMH_20750) comGF 4003093..4003563 (-) 471 WP_000923094.1 competence type IV pilus minor pilin ComGF -
  ACLIMH_RS20755 (ACLIMH_20755) comGE 4003533..4003835 (-) 303 WP_000829456.1 competence type IV pilus minor pilin ComGE -
  ACLIMH_RS20760 (ACLIMH_20760) comGD 4003828..4004283 (-) 456 WP_000810395.1 comG operon protein ComGD -
  ACLIMH_RS20765 (ACLIMH_20765) comGC 4004280..4004579 (-) 300 WP_001178696.1 comG operon protein ComGC -
  ACLIMH_RS20770 (ACLIMH_20770) comGB 4004591..4005622 (-) 1032 WP_000471911.1 comG operon protein ComGB -
  ACLIMH_RS20775 (ACLIMH_20775) comGA 4005615..4006658 (-) 1044 WP_001013211.1 competence protein ComGA Machinery gene
  ACLIMH_RS20780 (ACLIMH_20780) - 4006864..4007559 (+) 696 WP_000434117.1 helix-turn-helix transcriptional regulator -
  ACLIMH_RS20785 (ACLIMH_20785) - 4007685..4007927 (+) 243 WP_000440711.1 DUF2626 domain-containing protein -
  ACLIMH_RS20790 (ACLIMH_20790) - 4008035..4009429 (+) 1395 WP_001094329.1 L-cystine transporter -
  ACLIMH_RS20795 (ACLIMH_20795) - 4009605..4010012 (-) 408 WP_000877905.1 hypothetical protein -
  ACLIMH_RS20800 (ACLIMH_20800) - 4010100..4010315 (-) 216 WP_001008320.1 DUF3912 family protein -
  ACLIMH_RS20805 (ACLIMH_20805) - 4010568..4010879 (+) 312 WP_001093243.1 hypothetical protein -
  ACLIMH_RS20810 (ACLIMH_20810) - 4010915..4011403 (-) 489 WP_000764252.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39318.80 Da        Isoelectric Point: 9.0775

>NTDB_id=1085272 ACLIMH_RS20775 WP_001013211.1 4005615..4006658(-) (comGA) [Bacillus thuringiensis strain MPB12]
MNGIESFANTILKEACRVQASDLHIVPRQKDVVVQLRVGKDLMTKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKILLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1085272 ACLIMH_RS20775 WP_001013211.1 4005615..4006658(-) (comGA) [Bacillus thuringiensis strain MPB12]
ATGAATGGAATTGAAAGCTTTGCGAATACGATTTTGAAAGAAGCGTGCAGGGTGCAAGCGTCGGACTTACATATTGTACC
CCGGCAGAAGGATGTAGTGGTTCAACTGCGTGTAGGAAAAGATTTAATGACGAAACAATGCATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAAGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGATACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.213

100

0.582
  pilB Vibrio campbellii strain DS40M4

35.511

100

0.36

Multiple sequence alignment