Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACLIMC_RS25600 Genome accession   NZ_CP177035
Coordinates   4906545..4907894 (-) Length   449 a.a.
NCBI ID   WP_000879420.1    Uniprot ID   B7JFM3
Organism   Bacillus thuringiensis strain MPB08     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4901545..4912894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLIMC_RS25580 (ACLIMC_25580) secA 4901866..4904373 (-) 2508 WP_000579377.1 preprotein translocase subunit SecA -
  ACLIMC_RS25585 (ACLIMC_25585) hpf 4904650..4905192 (-) 543 WP_000671189.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACLIMC_RS25590 (ACLIMC_25590) cspC 4905517..4905714 (-) 198 WP_001990088.1 cold shock protein CspC -
  ACLIMC_RS25595 (ACLIMC_25595) comFC 4905841..4906545 (-) 705 WP_002157779.1 comF operon protein ComFC -
  ACLIMC_RS25600 (ACLIMC_25600) comFA 4906545..4907894 (-) 1350 WP_000879420.1 ATP-dependent helicase ComFA Machinery gene
  ACLIMC_RS25605 (ACLIMC_25605) - 4908022..4909332 (-) 1311 WP_000734329.1 NlpC/P60 family protein -
  ACLIMC_RS25610 (ACLIMC_25610) - 4909585..4909899 (-) 315 WP_000400857.1 winged helix-turn-helix transcriptional regulator -
  ACLIMC_RS25615 (ACLIMC_25615) - 4910073..4910915 (-) 843 WP_000844769.1 DegV family protein -
  ACLIMC_RS25620 (ACLIMC_25620) - 4911155..4911790 (+) 636 WP_000926685.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51161.82 Da        Isoelectric Point: 9.5824

>NTDB_id=1085011 ACLIMC_RS25600 WP_000879420.1 4906545..4907894(-) (comFA) [Bacillus thuringiensis strain MPB08]
MLAGKQLLLEELPSDLRRELSDLKKEGEVICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGGRVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRTGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEIGLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1085011 ACLIMC_RS25600 WP_000879420.1 4906545..4907894(-) (comFA) [Bacillus thuringiensis strain MPB08]
ATGCTCGCTGGAAAGCAGTTACTATTAGAAGAACTCCCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGGAGGG
AGAGGTCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGCTGGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAAAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGGAAGAGTGTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACGGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAACGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGCTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAGGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCATTTCGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(312-408)

Type III restriction enzyme, res subunit (ResIII)

(108-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7JFM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.914

93.096

0.483
  comFA Latilactobacillus sakei subsp. sakei 23K

40.704

88.641

0.361

Multiple sequence alignment