Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACLIMC_RS20935 Genome accession   NZ_CP177035
Coordinates   4040408..4041451 (-) Length   347 a.a.
NCBI ID   WP_001013197.1    Uniprot ID   A0AAE9TCD1
Organism   Bacillus thuringiensis strain MPB08     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4035408..4046451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLIMC_RS20890 (ACLIMC_20890) - 4035835..4036035 (-) 201 WP_000106085.1 YqzE family protein -
  ACLIMC_RS20895 (ACLIMC_20895) aroK 4036074..4036571 (-) 498 WP_000836643.1 shikimate kinase AroK -
  ACLIMC_RS20900 (ACLIMC_20900) - 4036692..4037342 (-) 651 WP_000183879.1 2OG-Fe(II) oxygenase -
  ACLIMC_RS20905 (ACLIMC_20905) comGG 4037518..4037889 (-) 372 WP_001231192.1 competence type IV pilus minor pilin ComGG -
  ACLIMC_RS20910 (ACLIMC_20910) comGF 4037886..4038356 (-) 471 WP_000923094.1 competence type IV pilus minor pilin ComGF -
  ACLIMC_RS20915 (ACLIMC_20915) comGE 4038326..4038628 (-) 303 WP_000829456.1 competence type IV pilus minor pilin ComGE -
  ACLIMC_RS20920 (ACLIMC_20920) comGD 4038621..4039076 (-) 456 WP_000810395.1 comG operon protein ComGD -
  ACLIMC_RS20925 (ACLIMC_20925) comGC 4039073..4039372 (-) 300 WP_001178696.1 comG operon protein ComGC -
  ACLIMC_RS20930 (ACLIMC_20930) comGB 4039384..4040415 (-) 1032 WP_411500949.1 comG operon protein ComGB -
  ACLIMC_RS20935 (ACLIMC_20935) comGA 4040408..4041451 (-) 1044 WP_001013197.1 competence protein ComGA Machinery gene
  ACLIMC_RS20940 (ACLIMC_20940) - 4041657..4042352 (+) 696 WP_000434117.1 helix-turn-helix transcriptional regulator -
  ACLIMC_RS20945 (ACLIMC_20945) - 4042478..4042720 (+) 243 WP_000440711.1 DUF2626 domain-containing protein -
  ACLIMC_RS20950 (ACLIMC_20950) - 4042828..4044222 (+) 1395 WP_001094329.1 L-cystine transporter -
  ACLIMC_RS20955 (ACLIMC_20955) - 4044460..4044675 (-) 216 WP_001008321.1 DUF3912 family protein -
  ACLIMC_RS20960 (ACLIMC_20960) - 4044928..4045239 (+) 312 WP_001093243.1 hypothetical protein -
  ACLIMC_RS20965 (ACLIMC_20965) - 4045276..4045764 (-) 489 WP_000764252.1 hypothetical protein -
  ACLIMC_RS20970 (ACLIMC_20970) - 4045844..4046218 (-) 375 WP_001012351.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39397.95 Da        Isoelectric Point: 9.0635

>NTDB_id=1085000 ACLIMC_RS20935 WP_001013197.1 4040408..4041451(-) (comGA) [Bacillus thuringiensis strain MPB08]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKYCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKILLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1085000 ACLIMC_RS20935 WP_001013197.1 4040408..4041451(-) (comGA) [Bacillus thuringiensis strain MPB08]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAATACTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCTATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGATACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATTGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579

Multiple sequence alignment