Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ACLINZ_RS05765 Genome accession   NZ_CP176860
Coordinates   1127264..1130989 (+) Length   1241 a.a.
NCBI ID   WP_411501188.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain MPB18     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1122264..1135989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLINZ_RS05750 (ACLINZ_05750) - 1122422..1123015 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  ACLINZ_RS05755 (ACLINZ_05755) lepB 1123072..1123635 (+) 564 WP_000751894.1 signal peptidase I -
  ACLINZ_RS05760 (ACLINZ_05760) addB 1123752..1127267 (+) 3516 WP_000058572.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACLINZ_RS05765 (ACLINZ_05765) addA 1127264..1130989 (+) 3726 WP_411501188.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACLINZ_RS05770 (ACLINZ_05770) - 1131002..1131289 (+) 288 WP_000718626.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  ACLINZ_RS05775 (ACLINZ_05775) gerPF 1131327..1131542 (-) 216 WP_001141566.1 spore germination protein GerPF -
  ACLINZ_RS05780 (ACLINZ_05780) - 1131585..1131971 (-) 387 WP_000902341.1 spore germination protein GerPE -
  ACLINZ_RS05785 (ACLINZ_05785) gerPD 1131987..1132181 (-) 195 WP_001052802.1 spore germination protein GerPD -
  ACLINZ_RS05790 (ACLINZ_05790) gerPC 1132188..1132802 (-) 615 WP_001070767.1 spore germination protein GerPC -
  ACLINZ_RS05795 (ACLINZ_05795) gerPB 1132870..1133076 (-) 207 WP_001012512.1 spore germination protein GerPB -
  ACLINZ_RS05800 (ACLINZ_05800) gerPA 1133091..1133312 (-) 222 WP_001111188.1 spore germination protein GerPA -
  ACLINZ_RS05805 (ACLINZ_05805) - 1133409..1133588 (-) 180 WP_000462851.1 aspartyl-phosphate phosphatase Spo0E family protein -
  ACLINZ_RS05810 (ACLINZ_05810) - 1133836..1134735 (+) 900 WP_038357215.1 fumarylacetoacetate hydrolase family protein -
  ACLINZ_RS05815 (ACLINZ_05815) - 1134771..1135055 (-) 285 WP_000925338.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142774.22 Da        Isoelectric Point: 4.8876

>NTDB_id=1083349 ACLINZ_RS05765 WP_411501188.1 1127264..1130989(+) (addA) [Bacillus thuringiensis strain MPB18]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNMIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSAAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVKYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHVRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWKYRYGEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAVEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=1083349 ACLINZ_RS05765 WP_411501188.1 1127264..1130989(+) (addA) [Bacillus thuringiensis strain MPB18]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGAGAGGCTTTAGAAAAAGTATTAATTGATGAGCCTGGCTCTCAGCACATCCGAAAGCAACTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACCGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATATGATATTCTTTGAACTCGTTGATCGTTATACGAGCGACCGTAGTGATGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTAAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACTGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACGCTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCAGCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAACAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCACGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTAAAATA
CCGTGACTTCGTTATTTTACTTCGCTCGATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGACCTTGCCACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCACGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTCGCTGGACTTGGTCGTCGTTTTAATACGCAAGACTTAATGAA
ACGTTTCTTACTTCATAAAGACTTTGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGG
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCACGTACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGTATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGGAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTTCCGTTAGAGAGTGAACGGAAAGAAGAAGTGTACGATAGATTAATGTGGAAGTACAGATATGGAG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAGAACACGTCCTCGTTTCATGGAAAAAAAAGGGTTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAGTTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCGGGAGGATTCGAACAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1059-1232)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.666

100

0.537

Multiple sequence alignment