Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AACH77_RS27160 Genome accession   NZ_AP029053
Coordinates   1814158..1815663 (+) Length   501 a.a.
NCBI ID   WP_027810400.1    Uniprot ID   A0A3N8YL34
Organism   Burkholderia contaminans strain GalA64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1809158..1820663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH77_RS27135 (BconGalA64_53820) kdsD 1810389..1811372 (+) 984 WP_006483136.1 arabinose 5-phosphate isomerase KdsD -
  AACH77_RS27140 (BconGalA64_53830) - 1811372..1811908 (+) 537 WP_006477791.1 HAD family hydrolase -
  AACH77_RS27145 (BconGalA64_53840) lptC 1811912..1812526 (+) 615 WP_124592226.1 LPS export ABC transporter periplasmic protein LptC -
  AACH77_RS27150 (BconGalA64_53850) lptA 1812547..1813212 (+) 666 WP_006477789.1 lipopolysaccharide transport periplasmic protein LptA -
  AACH77_RS27155 (BconGalA64_53860) lptB 1813209..1813985 (+) 777 WP_006477788.1 LPS export ABC transporter ATP-binding protein -
  AACH77_RS27160 (BconGalA64_53870) rpoN/rpoN1 1814158..1815663 (+) 1506 WP_027810400.1 RNA polymerase factor sigma-54 Machinery gene
  AACH77_RS27165 (BconGalA64_53880) raiA 1815822..1816181 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  AACH77_RS27170 (BconGalA64_53890) - 1816376..1816888 (+) 513 WP_027806888.1 PTS sugar transporter subunit IIA -
  AACH77_RS27175 (BconGalA64_53900) hprK 1817018..1817986 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  AACH77_RS27180 (BconGalA64_53910) rapZ 1818061..1818969 (+) 909 WP_006477783.1 RNase adapter RapZ -
  AACH77_RS27185 (BconGalA64_53920) - 1818997..1819632 (+) 636 WP_011546241.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54739.80 Da        Isoelectric Point: 4.8740

>NTDB_id=108317 AACH77_RS27160 WP_027810400.1 1814158..1815663(+) (rpoN/rpoN1) [Burkholderia contaminans strain GalA64]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSTSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=108317 AACH77_RS27160 WP_027810400.1 1814158..1815663(+) (rpoN/rpoN1) [Burkholderia contaminans strain GalA64]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCCAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCACCGCG
ACCAACGGCAGCAGCACGTCCAGCGATCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGTTACGGGTC
CGGTGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATACGGCCGCTCGAGCGGTGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGATCATCTCTCGGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTACT
CGCCGACCTGCCGCCCGAACTGGAAATCGACGGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGCTGCGTCTCGATCCGTCCCCCACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTTCCCGGCGCCGCCTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCTGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAACAGCA
GCTCCAGGAAGCACGCTGGTTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCACAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3N8YL34

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment