Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACK3B6_RS13205 Genome accession   NZ_CP176792
Coordinates   2599791..2600216 (-) Length   141 a.a.
NCBI ID   WP_105953566.1    Uniprot ID   -
Organism   Bacillus halotolerans strain BCP32     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2594791..2605216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3B6_RS13175 (ACK3B6_13175) - 2595046..2595180 (-) 135 WP_010335019.1 hypothetical protein -
  ACK3B6_RS13180 (ACK3B6_13180) - 2595258..2595989 (-) 732 WP_101864558.1 SGNH/GDSL hydrolase family protein -
  ACK3B6_RS13185 (ACK3B6_13185) - 2596236..2596988 (-) 753 WP_416042757.1 N-acetylmuramoyl-L-alanine amidase -
  ACK3B6_RS13190 (ACK3B6_13190) - 2597183..2597809 (+) 627 WP_202852917.1 TVP38/TMEM64 family protein -
  ACK3B6_RS13195 (ACK3B6_13195) gnd 2597830..2598723 (-) 894 WP_202852916.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACK3B6_RS13200 (ACK3B6_13200) - 2599029..2599757 (+) 729 WP_248337398.1 DUF308 domain-containing protein -
  ACK3B6_RS13205 (ACK3B6_13205) nucA/comI 2599791..2600216 (-) 426 WP_105953566.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACK3B6_RS13210 (ACK3B6_13210) - 2600393..2600743 (+) 351 Protein_2553 sigma-70 family RNA polymerase sigma factor -
  ACK3B6_RS13215 (ACK3B6_13215) spoIVCA 2600771..2602231 (-) 1461 WP_250620044.1 site-specific DNA recombinase SpoIVCA -
  ACK3B6_RS13220 (ACK3B6_13220) - 2602189..2602367 (-) 179 Protein_2555 hypothetical protein -
  ACK3B6_RS13225 (ACK3B6_13225) - 2603280..2603492 (+) 213 WP_029726695.1 spore germination protein -
  ACK3B6_RS13230 (ACK3B6_13230) - 2603860..2603953 (+) 94 Protein_2557 spore germination protein -
  ACK3B6_RS13235 (ACK3B6_13235) - 2604237..2604947 (-) 711 WP_029726694.1 DUF421 domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15589.77 Da        Isoelectric Point: 5.7459

>NTDB_id=1083013 ACK3B6_RS13205 WP_105953566.1 2599791..2600216(-) (nucA/comI) [Bacillus halotolerans strain BCP32]
MKRWMAMLFLAAAVFLCLLGKDMTAPQQVKGAALYDKILYFPLSRYPETGDHIRDAIAQGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1083013 ACK3B6_RS13205 WP_105953566.1 2599791..2600216(-) (nucA/comI) [Bacillus halotolerans strain BCP32]
ATGAAAAGATGGATGGCAATGCTTTTTCTGGCGGCAGCAGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGCGATCATATAA
GAGATGCGATTGCCCAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAATCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGTGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCAGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.484

87.943

0.532

Multiple sequence alignment