Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACK3B6_RS01970 Genome accession   NZ_CP176792
Coordinates   369667..370104 (-) Length   145 a.a.
NCBI ID   WP_024120178.1    Uniprot ID   -
Organism   Bacillus halotolerans strain BCP32     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 364667..375104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3B6_RS01935 (ACK3B6_01935) - 365336..365674 (+) 339 Protein_337 alcohol dehydrogenase catalytic domain-containing protein -
  ACK3B6_RS01940 (ACK3B6_01940) - 365737..366066 (+) 330 WP_105991526.1 YckD family protein -
  ACK3B6_RS01945 (ACK3B6_01945) - 366207..366626 (+) 420 WP_024120173.1 MarR family winged helix-turn-helix transcriptional regulator -
  ACK3B6_RS01950 (ACK3B6_01950) - 366617..366955 (+) 339 WP_202852453.1 DUF3147 family protein -
  ACK3B6_RS01955 (ACK3B6_01955) - 366972..367700 (+) 729 WP_069487486.1 AAA family ATPase -
  ACK3B6_RS01960 (ACK3B6_01960) - 367773..369206 (+) 1434 WP_101860166.1 glycoside hydrolase family 1 protein -
  ACK3B6_RS01965 (ACK3B6_01965) nin/comJ 369242..369640 (-) 399 WP_105955349.1 competence protein ComJ Regulator
  ACK3B6_RS01970 (ACK3B6_01970) nucA/comI 369667..370104 (-) 438 WP_024120178.1 DNA-entry nuclease Machinery gene
  ACK3B6_RS01975 (ACK3B6_01975) tlpC 370290..372014 (-) 1725 WP_202852454.1 methyl-accepting chemotaxis protein TlpC -
  ACK3B6_RS01980 (ACK3B6_01980) hxlB 372127..372684 (-) 558 WP_105991524.1 6-phospho-3-hexuloisomerase -
  ACK3B6_RS01985 (ACK3B6_01985) hxlA 372690..373322 (-) 633 WP_202852455.1 3-hexulose-6-phosphate synthase -
  ACK3B6_RS01990 (ACK3B6_01990) hxlR 373554..373916 (+) 363 WP_003241118.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15973.68 Da        Isoelectric Point: 4.1966

>NTDB_id=1082967 ACK3B6_RS01970 WP_024120178.1 369667..370104(-) (nucA/comI) [Bacillus halotolerans strain BCP32]
MDIIKTLLLVIVVIAAAAVGLIKGDFFSDDQKTSQTEEYDETLAFPSERYPETANHIKDAISEGHSDVCTIDRDGAEERR
EQSLKDVPSKTGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1082967 ACK3B6_RS01970 WP_024120178.1 369667..370104(-) (nucA/comI) [Bacillus halotolerans strain BCP32]
ATGGACATTATAAAAACTTTACTTCTCGTCATAGTCGTCATAGCAGCTGCAGCTGTTGGCCTGATTAAGGGAGACTTTTT
CTCAGACGATCAAAAAACGTCTCAAACAGAAGAATATGACGAAACACTGGCCTTCCCTTCTGAGCGTTACCCTGAGACAG
CAAATCATATCAAGGACGCAATAAGTGAGGGGCATTCAGATGTGTGCACCATAGACAGAGACGGAGCTGAGGAACGCCGC
GAACAATCATTAAAAGACGTACCTTCCAAAACAGGGTATGACCGAGATGAATGGCCAATGGCCATGTGCAAAGAAGGCGG
TGAAGGGGCTTCTGTAGAATATATTTCTCCCGCTGACAATCGCGGCGCAGGCTCCTGGGTCGGGCATCAGCTGACCGATT
ACCCTGACGGCACAAAGGTTTTATTTACGATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

92.361

99.31

0.917

Multiple sequence alignment