Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   ACK3B6_RS00020 Genome accession   NZ_CP176792
Coordinates   3031..4143 (+) Length   370 a.a.
NCBI ID   WP_024123582.1    Uniprot ID   -
Organism   Bacillus halotolerans strain BCP32     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1532..28051 3031..4143 within 0


Gene organization within MGE regions


Location: 1532..28051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3B6_RS00010 (ACK3B6_00010) dnaN 1532..2668 (+) 1137 WP_105954685.1 DNA polymerase III subunit beta -
  ACK3B6_RS00015 (ACK3B6_00015) rlbA 2800..3015 (+) 216 WP_003219264.1 ribosome maturation protein RlbA -
  ACK3B6_RS00020 (ACK3B6_00020) recF 3031..4143 (+) 1113 WP_024123582.1 DNA replication/repair protein RecF Machinery gene
  ACK3B6_RS00025 (ACK3B6_00025) remB 4161..4406 (+) 246 WP_024123583.1 extracellular matrix regulator RemB -
  ACK3B6_RS00030 (ACK3B6_00030) gyrB 4462..6378 (+) 1917 WP_024123584.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  ACK3B6_RS00035 (ACK3B6_00035) gyrA 6590..9061 (+) 2472 WP_202853894.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -
  ACK3B6_RS00065 (ACK3B6_00065) - 14380..15330 (-) 951 WP_202853799.1 YaaC family protein -
  ACK3B6_RS00070 (ACK3B6_00070) guaB 15451..16917 (+) 1467 WP_024123587.1 IMP dehydrogenase -
  ACK3B6_RS00075 (ACK3B6_00075) dacA 17072..18403 (+) 1332 WP_024123588.1 D-alanyl-D-alanine carboxypeptidase -
  ACK3B6_RS00080 (ACK3B6_00080) pdxS 18599..19483 (+) 885 WP_024123589.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  ACK3B6_RS00085 (ACK3B6_00085) pdxT 19504..20094 (+) 591 WP_024123590.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  ACK3B6_RS00090 (ACK3B6_00090) serS 20426..21703 (+) 1278 WP_024123591.1 serine--tRNA ligase -
  ACK3B6_RS00100 (ACK3B6_00100) dck 22043..22696 (-) 654 WP_029441179.1 deoxyadenosine/deoxycytidine kinase -
  ACK3B6_RS00105 (ACK3B6_00105) dgk 22693..23316 (-) 624 WP_044153628.1 deoxyguanosine kinase -
  ACK3B6_RS00110 (ACK3B6_00110) - 23415..24704 (-) 1290 WP_105955734.1 glycoside hydrolase family 18 protein -
  ACK3B6_RS00115 (ACK3B6_00115) - 24761..25312 (-) 552 WP_010332741.1 isochorismatase family cysteine hydrolase -
  ACK3B6_RS00120 (ACK3B6_00120) tadA 25396..25881 (+) 486 WP_024123596.1 tRNA adenosine(34) deaminase TadA -
  ACK3B6_RS00130 (ACK3B6_00130) dnaX 26357..28051 (+) 1695 WP_202853798.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42434.44 Da        Isoelectric Point: 7.0882

>NTDB_id=1082960 ACK3B6_RS00020 WP_024123582.1 3031..4143(+) (recF) [Bacillus halotolerans strain BCP32]
MYIQNLELTSYRNYEHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLYDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLIEVAAKVVVKRLQFTAQLEKWAQPIHSGISRGLEELTLKYHTALEVSDPEDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGTLVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1082960 ACK3B6_RS00020 WP_024123582.1 3031..4143(+) (recF) [Bacillus halotolerans strain BCP32]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTATGAACATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATTGGAGAAAATGCTCAGGGGAAAACAAACCTCATGGAAGCGATCTATGTCTTGTCCATGGCAAAATCGCATCGGA
CTTCAAATGACAAAGAACTTATCCGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGAGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATCGAACAGCAAAAGCTCAGCCAGTA
TGTCGGCGCCCTCAACACCATCATGTTTGCACCGGAAGATTTAAACCTTGTAAAGGGAAGCCCTCAAGTGAGACGGAGAT
TTCTTGACATGGAAATCGGCCAAGTTTCTCCCGTCTACCTGTATGACCTTTCTCTTTACCAGAAGATTCTTTCCCAGCGA
AATCATTTTTTGAAGCAGCTGCAAACAAGAAAACAAACTGACAGAACAATGCTTGATGTTCTGACTGATCAGCTTATTGA
AGTTGCCGCAAAAGTCGTCGTAAAACGTCTGCAGTTCACGGCACAGCTCGAAAAATGGGCGCAGCCTATCCATTCGGGCA
TCTCAAGAGGGCTTGAGGAATTGACGCTGAAGTACCATACGGCGCTTGAGGTATCAGATCCTGAAGACTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATCGAGCGCGGCGTCACGCTGTCTGGACCTCATCG
GGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGGTCTCAAGGACAGCAGCGGACGACGGCATTAT
CCCTTAAGCTGGCTGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTGCTGAGT
GAGCTGGATGATTATCGCCAATCGCACTTGCTTCATACCATCCAGGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGGCAAGCAGGAATGTTCCGTGTGCAAAACGGTACGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-365)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

98.108

100

0.981

Multiple sequence alignment