Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACK2VR_RS02685 Genome accession   NZ_CP176717
Coordinates   568627..570060 (-) Length   477 a.a.
NCBI ID   WP_058609398.1    Uniprot ID   A0AAP8NTY1
Organism   Enterobacter cancerogenus strain GTVSS-029     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 563627..575060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2VR_RS02655 (ACK2VR_02655) elbB 565159..565812 (+) 654 WP_153689314.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACK2VR_RS02660 (ACK2VR_02660) mtgA 565809..566534 (+) 726 WP_153689315.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACK2VR_RS02665 (ACK2VR_02665) npr 566574..566846 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  ACK2VR_RS02670 (ACK2VR_02670) rapZ 566843..567697 (-) 855 WP_006178792.1 RNase adapter RapZ -
  ACK2VR_RS02675 (ACK2VR_02675) ptsN 567743..568234 (-) 492 WP_006178791.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACK2VR_RS02680 (ACK2VR_02680) hpf 568317..568604 (-) 288 WP_006178790.1 ribosome hibernation promoting factor -
  ACK2VR_RS02685 (ACK2VR_02685) rpoN/rpoN1 568627..570060 (-) 1434 WP_058609398.1 RNA polymerase factor sigma-54 Machinery gene
  ACK2VR_RS02690 (ACK2VR_02690) lptB 570108..570833 (-) 726 WP_006178788.1 LPS export ABC transporter ATP-binding protein -
  ACK2VR_RS02695 (ACK2VR_02695) lptA 570840..571394 (-) 555 WP_006178787.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACK2VR_RS02700 (ACK2VR_02700) lptC 571363..571938 (-) 576 WP_006178786.1 LPS export ABC transporter periplasmic protein LptC -
  ACK2VR_RS02705 (ACK2VR_02705) kdsC 571935..572501 (-) 567 WP_058609399.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACK2VR_RS02710 (ACK2VR_02710) kdsD 572516..573502 (-) 987 WP_006178784.1 arabinose-5-phosphate isomerase KdsD -
  ACK2VR_RS02715 (ACK2VR_02715) - 573516..574493 (-) 978 WP_153689316.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54129.99 Da        Isoelectric Point: 4.3332

>NTDB_id=1082657 ACK2VR_RS02685 WP_058609398.1 568627..570060(-) (rpoN/rpoN1) [Enterobacter cancerogenus strain GTVSS-029]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQTQDAEMLDTVDALEQKEMPD
ELPLDASWDELYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESIGDEDVELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNVIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1082657 ACK2VR_RS02685 WP_058609398.1 568627..570060(-) (rpoN/rpoN1) [Enterobacter cancerogenus strain GTVSS-029]
ATGAAGCAAGGTTTGCAATTAAGGCTGAGCCAGCAACTGGCGATGACGCCGCAGCTCCAGCAGGCGATCCGTCTGCTGCA
GCTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGTTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACACCCAGCAAACGCAAGACGCAGAAATGCTCGACACCGTTGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAACTCTACACCGCCGGTACCCCTTCCGGAACGCGTGCAGACTACCAGGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACCACCCAGTCTCTGCAGGATTACCTGATGTGGCAGGTGGAGCTTACGCCGTTCT
CCGATACCGATCGCGCCATTGCGACCTCCATTGTCGATGCCGTGGATGACACAGGCTATCTGACCGTCACGCTGGACGAC
ATTCTGGAAAGTATCGGCGACGAAGACGTTGAGCTGGAAGAAATTGAAGCCGTGTTAAAGCGCATCCAGCGCTTCGACCC
GGTCGGTGTGGCGGCGAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCGCAGTTCGCCAAAGAGACGCCGTGGATCG
ACGAAGCCCGCTTAATCATCAGCGATCATCTGGATCTGCTCGCCAACCATGACTTCCGTTCGCTGATGCGCGTCACGCGT
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTGAATGTCATTCAGTCCCTCGATCCGCGTCCCGGCCAGTCGATCCAGACCAG
CGAACCGGAATACGTGATCCCTGACGTGCTGGTGCGAAAGCATAACGGTCGCTGGGTGGTTGAACTGAATTCAGACAGCA
TCCCTCGTTTGCAAATTAACCAGCAGTACGCCTCCATGTGCACCAGCGTTCGCAACGACTCCGACAATCAATATATTCGT
AGCAACCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTCGAGCAGGGCGAAGAATTTATGAAGCCGATGGTGCTGGCAGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCAATCCGTGCACTGGTGAAGAAGTT
GATCGCCGCGGAAAACCCCGCGAAGCCACTGAGCGACAGTAAGTTGACCACCCTTCTCTCCGATCAGGGTATTATGGTGG
CTCGTCGTACCGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482

Multiple sequence alignment