Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACK2VN_RS27490 Genome accession   NZ_CP176690
Coordinates   5250996..5252372 (-) Length   458 a.a.
NCBI ID   WP_001085212.1    Uniprot ID   A0A2B3Y2M8
Organism   Bacillus paranthracis strain GTVSS-022     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5245996..5257372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2VN_RS27470 (ACK2VN_27470) ispF 5247360..5247836 (-) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACK2VN_RS27475 (ACK2VN_27475) ispD 5247952..5248632 (-) 681 WP_000288304.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACK2VN_RS27480 (ACK2VN_27480) - 5248649..5249758 (-) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  ACK2VN_RS27485 (ACK2VN_27485) disA 5249919..5250992 (-) 1074 WP_000392171.1 DNA integrity scanning diadenylate cyclase DisA -
  ACK2VN_RS27490 (ACK2VN_27490) radA 5250996..5252372 (-) 1377 WP_001085212.1 DNA repair protein RadA Machinery gene
  ACK2VN_RS27495 (ACK2VN_27495) clpC 5252469..5254904 (-) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  ACK2VN_RS27500 (ACK2VN_27500) - 5254927..5255991 (-) 1065 WP_000050825.1 protein arginine kinase -
  ACK2VN_RS27505 (ACK2VN_27505) - 5255996..5256544 (-) 549 WP_000128382.1 UvrB/UvrC motif-containing protein -
  ACK2VN_RS27510 (ACK2VN_27510) ctsR 5256718..5257179 (-) 462 WP_001244563.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49858.44 Da        Isoelectric Point: 7.1421

>NTDB_id=1082396 ACK2VN_RS27490 WP_001085212.1 5250996..5252372(-) (radA) [Bacillus paranthracis strain GTVSS-022]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=1082396 ACK2VN_RS27490 WP_001085212.1 5250996..5252372(-) (radA) [Bacillus paranthracis strain GTVSS-022]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCAAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCGAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTTCAAGAATTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCCTTAGTACTGATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCACAATTAGCGGATTCTTCATATGATGTCCTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGACTTACAGCGAATTGCA
ACACATATTGAAGAGGTGAACCCAGCCTTTGTTGTTATTGATTCTATTCAAACAATACATCTACCTGAAGTAACATCAGC
ACCGGGAAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATACCGCATTTTGCGAGCAGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAGCTTGGCCTTGCAGAAGTATTAAACCCTTCTGAAATTTTTCTTGAGGAAAGACCAGTTGGGGTTGCAG
GATCAACAGTGGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATTTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGATGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGCGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCACTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B3Y2M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629

Multiple sequence alignment