Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AACH55_RS20470 Genome accession   NZ_AP029028
Coordinates   4524551..4526041 (+) Length   496 a.a.
NCBI ID   WP_338716459.1    Uniprot ID   -
Organism   Herbaspirillum sp. DW155     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4519551..4531041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH55_RS20445 (HBDW_40510) - 4520871..4521899 (+) 1029 WP_338716454.1 KpsF/GutQ family sugar-phosphate isomerase -
  AACH55_RS20450 (HBDW_40520) - 4521899..4522453 (+) 555 WP_338716455.1 HAD family hydrolase -
  AACH55_RS20455 (HBDW_40530) lptC 4522450..4523058 (+) 609 WP_338716456.1 LPS export ABC transporter periplasmic protein LptC -
  AACH55_RS20460 (HBDW_40540) lptA 4523110..4523736 (+) 627 WP_338716457.1 lipopolysaccharide transport periplasmic protein LptA -
  AACH55_RS20465 (HBDW_40550) lptB 4523739..4524482 (+) 744 WP_338716458.1 LPS export ABC transporter ATP-binding protein -
  AACH55_RS20470 (HBDW_40560) rpoN/rpoN1 4524551..4526041 (+) 1491 WP_338716459.1 RNA polymerase factor sigma-54 Machinery gene
  AACH55_RS20475 (HBDW_40570) raiA 4526195..4526557 (+) 363 WP_338716460.1 ribosome-associated translation inhibitor RaiA -
  AACH55_RS20480 (HBDW_40580) grxD 4526939..4527250 (+) 312 WP_017452551.1 Grx4 family monothiol glutaredoxin -
  AACH55_RS20485 (HBDW_40590) - 4527275..4527877 (+) 603 WP_338716461.1 UbiX family flavin prenyltransferase -
  AACH55_RS20490 (HBDW_40600) - 4527958..4528725 (-) 768 WP_338716462.1 SDR family oxidoreductase -
  AACH55_RS20495 (HBDW_40610) - 4529288..4530193 (+) 906 WP_338716463.1 DUF6662 family protein -
  AACH55_RS20500 (HBDW_40620) - 4530261..4530800 (+) 540 WP_338716464.1 FMN-binding protein -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55137.62 Da        Isoelectric Point: 5.0403

>NTDB_id=108198 AACH55_RS20470 WP_338716459.1 4524551..4526041(+) (rpoN/rpoN1) [Herbaspirillum sp. DW155]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQILSDNPLLERLDDALDNSVRLLADGAVSSSPSSGVADTSGE
GNAEPAPAAADGDSSFDYQDNNPENGVTGEADWSFDDVARSGKSPDDDDARPQLEAHELTLREHLLEQIRVTARTQRDRA
LLELLTDALDESGYLEEPLEDILARLPEELGIDMEELSISLKLLQSLDPAGVGARSAGECLALQIRRLPKIPFVTRKLAL
RIVEDYLPLFAQRDFNKIKKALDCDDEDLREAQAVIRQCRPHPGADFARDASDYVVPDVIVKQTKNGWQVMLNHEVMPKL
RVNAMYASALKQAKGEGSLSSQLQEAKWLIKNMRQRFDTILRVAQAIVERQRNFFSHGAVAMRPLVLREIADTLGLHEST
ISRVTTQKYMLTPHGMFELKYFFGSHVATETGGEASSTAIRALIKQLIGAENPQTPLSDSKIADMLAEQGMVVARRTVAK
YREVLKIPPVNLRKSL

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=108198 AACH55_RS20470 WP_338716459.1 4524551..4526041(+) (rpoN/rpoN1) [Herbaspirillum sp. DW155]
ATGAAACAGTCTCTCCAGTTACGTACCTCGCAGCACCTCGCATTGACGCCGCAACTGCAGCAGTCGATCAGACTGTTGCA
ACTGTCGACGCTGGAACTGCACCAGGAACTGGAACAGATCCTCTCGGACAATCCCTTGCTGGAGCGGCTCGACGATGCGC
TCGACAATTCGGTGCGCCTGCTGGCCGATGGCGCGGTCTCCAGCTCGCCCTCCAGCGGCGTAGCCGACACCTCTGGCGAA
GGCAATGCCGAGCCGGCACCGGCAGCCGCCGATGGCGATTCCAGTTTTGACTACCAGGACAACAATCCCGAGAACGGCGT
CACCGGTGAGGCCGACTGGAGCTTCGATGATGTGGCCCGCAGCGGCAAGTCGCCTGACGACGACGACGCCCGCCCGCAAC
TCGAAGCCCACGAACTGACCCTGCGCGAGCACCTACTGGAACAGATCCGCGTGACCGCACGCACCCAGCGCGACCGCGCC
CTGCTGGAGTTGCTCACCGATGCGCTCGACGAGAGCGGCTATCTCGAAGAGCCGCTGGAAGACATCCTCGCGCGCCTGCC
CGAAGAACTGGGCATCGACATGGAAGAGCTGTCGATTTCCCTGAAGCTGCTGCAGAGCCTGGACCCGGCCGGCGTGGGTG
CGCGCAGTGCCGGTGAGTGCCTGGCCCTGCAGATCCGTCGCCTGCCCAAGATCCCCTTCGTCACGCGCAAGCTGGCGCTG
CGCATCGTGGAAGATTACCTGCCGCTGTTCGCGCAGCGCGACTTCAACAAGATCAAGAAAGCCCTCGATTGCGATGACGA
AGACCTGCGCGAAGCCCAGGCCGTGATCCGCCAGTGCCGTCCGCATCCGGGCGCCGATTTCGCGCGCGATGCCTCGGACT
ATGTGGTGCCGGACGTGATTGTCAAACAAACAAAGAACGGCTGGCAGGTCATGCTCAACCATGAGGTCATGCCCAAGCTG
CGTGTCAATGCGATGTATGCCAGCGCGCTGAAGCAGGCCAAGGGAGAAGGATCGCTGTCGTCGCAGCTGCAGGAAGCCAA
GTGGCTCATCAAGAATATGCGCCAGCGTTTCGACACGATTTTGCGTGTCGCACAGGCTATTGTGGAACGTCAAAGAAACT
TCTTTTCACATGGGGCAGTGGCCATGCGCCCCCTTGTGCTGCGTGAAATTGCTGATACACTGGGTCTACACGAGAGCACC
ATCTCTCGCGTGACAACTCAGAAATACATGCTCACGCCGCACGGCATGTTTGAGTTGAAGTACTTCTTCGGTAGCCACGT
CGCGACTGAAACCGGGGGCGAAGCCTCCTCCACCGCGATACGGGCGCTGATCAAACAGTTGATAGGAGCCGAGAACCCGC
AGACCCCATTATCCGATAGCAAGATTGCGGATATGCTGGCAGAACAAGGCATGGTGGTCGCGCGACGCACAGTCGCCAAA
TACCGCGAAGTGTTGAAGATTCCGCCGGTCAATCTCCGCAAGTCCCTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(339-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

59.252

100

0.607

Multiple sequence alignment