Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACK2JE_RS16715 Genome accession   NZ_CP176625
Coordinates   3472873..3474234 (-) Length   453 a.a.
NCBI ID   WP_411327907.1    Uniprot ID   -
Organism   Eubacterium callanderi strain SFAD75     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3467873..3479234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2JE_RS16680 scfA 3468024..3468164 (-) 141 WP_013381915.1 six-cysteine ranthipeptide SCIFF -
  ACK2JE_RS16685 yajC 3468190..3468633 (-) 444 WP_073381886.1 preprotein translocase subunit YajC -
  ACK2JE_RS16690 tgt 3468652..3469761 (-) 1110 WP_013381917.1 tRNA guanosine(34) transglycosylase Tgt -
  ACK2JE_RS16695 queA 3469774..3470808 (-) 1035 WP_073381889.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  ACK2JE_RS16700 ruvB 3470819..3471847 (-) 1029 WP_013381919.1 Holliday junction branch migration DNA helicase RuvB -
  ACK2JE_RS16705 ruvA 3471859..3472470 (-) 612 WP_013381920.1 Holliday junction branch migration protein RuvA -
  ACK2JE_RS16715 radA 3472873..3474234 (-) 1362 WP_411327907.1 DNA repair protein RadA Machinery gene
  ACK2JE_RS16720 - 3474245..3474730 (-) 486 WP_013381922.1 VanZ family protein -
  ACK2JE_RS16725 - 3474799..3475848 (-) 1050 WP_073381894.1 PTS transporter subunit IIC -
  ACK2JE_RS16730 - 3476116..3476757 (-) 642 WP_073381897.1 acetolactate decarboxylase -
  ACK2JE_RS16735 - 3476767..3477687 (-) 921 WP_083591059.1 CobW family GTP-binding protein -
  ACK2JE_RS16740 - 3477746..3479095 (-) 1350 WP_073381902.1 uroporphyrinogen decarboxylase family protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50478.83 Da        Isoelectric Point: 8.3489

>NTDB_id=1081612 ACK2JE_RS16715 WP_411327907.1 3472873..3474234(-) (radA) [Eubacterium callanderi strain SFAD75]
MAKIKKKFVCQNCGYSTPKWMGRCTECGEWNSFVEELDMPTEQGKKNTLERAVYTKPKRIEEIIPQKEDRFKTKNKELDR
VLGGGIVPGGVILLGGDPGIGKSTILLQTTENLGSQGLKILYISGEESEQQLKMRAVRMKVKSQNIFFLSEINVPYIVDT
ILHEKPDLVIIDSIQTMYSPTITSAPGSASQIRENANVLMQIAKKDNISMILVGHVTKEGNIAGPRVLEHMVDTVLYFEG
EKYHTYRILRGVKNRFGSTNEIGIFEMAQDGLHEVANPSEMMLSSRPKNTCGSVVVPCMEGTRPLLIELQGLVSQTSFGN
PRRMATGMDYNRMVLLLAIMEKRLGLQLQNLDAYINVVGGMKIDEPALDLAVVSVLYSSFRNFEIPEDMMIIGEVGLTGE
VRNIQHIEKRLKEGAKLGFKRCIMPKGNARGLENTGLELLPVDNISKALNILK

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1081612 ACK2JE_RS16715 WP_411327907.1 3472873..3474234(-) (radA) [Eubacterium callanderi strain SFAD75]
ATGGCGAAAATAAAAAAGAAATTTGTGTGCCAGAACTGCGGGTACAGCACGCCTAAATGGATGGGGCGCTGTACAGAATG
TGGCGAATGGAACTCCTTTGTTGAGGAGCTGGATATGCCCACTGAGCAGGGAAAGAAGAATACCCTTGAACGGGCGGTGT
ACACAAAACCCAAGCGTATTGAAGAAATTATTCCTCAGAAAGAGGATCGTTTTAAAACGAAGAATAAGGAGCTCGACCGC
GTCCTCGGCGGCGGCATTGTGCCTGGAGGCGTTATCCTTCTAGGAGGTGACCCGGGAATTGGAAAGTCAACCATTTTGCT
GCAGACGACGGAAAATCTTGGAAGCCAGGGGCTTAAAATCCTGTATATTTCAGGTGAGGAGTCCGAGCAGCAGCTCAAAA
TGCGCGCAGTCCGGATGAAGGTGAAATCCCAGAATATTTTTTTTCTGTCTGAGATTAACGTGCCCTATATCGTAGATACT
ATTTTGCATGAAAAGCCCGATCTGGTCATTATTGACTCGATTCAGACAATGTACAGTCCTACAATTACCTCAGCGCCCGG
AAGCGCCAGCCAGATAAGGGAGAACGCTAACGTGTTGATGCAGATTGCCAAAAAAGACAATATTTCGATGATTCTGGTCG
GACATGTGACCAAGGAAGGCAATATTGCCGGTCCCCGTGTGTTAGAACATATGGTTGATACGGTTTTGTATTTTGAGGGT
GAAAAGTACCATACCTACCGGATACTCAGAGGGGTCAAAAACCGTTTTGGCTCAACCAATGAGATCGGTATTTTCGAAAT
GGCGCAGGATGGCCTGCACGAGGTGGCAAATCCCTCTGAAATGATGCTGTCGAGCCGGCCGAAAAATACCTGTGGCTCTG
TGGTAGTGCCCTGCATGGAGGGGACACGTCCGCTTTTAATCGAGCTTCAGGGGCTCGTATCACAGACAAGCTTCGGTAAC
CCAAGGCGTATGGCCACCGGAATGGACTATAACCGTATGGTGCTGCTCCTGGCCATTATGGAAAAAAGGCTAGGATTACA
GCTTCAAAATCTTGATGCGTATATCAATGTGGTCGGGGGTATGAAGATAGATGAGCCGGCTCTTGATCTGGCCGTTGTGT
CTGTGCTATACTCAAGCTTCAGGAATTTTGAAATTCCAGAGGATATGATGATTATCGGCGAGGTAGGCCTGACTGGTGAG
GTCAGAAATATACAGCATATTGAAAAGCGCCTTAAAGAAGGCGCGAAGCTTGGCTTTAAGCGCTGTATCATGCCAAAGGG
CAATGCCCGGGGCCTGGAAAATACAGGGCTGGAGCTTCTGCCAGTCGATAATATATCTAAGGCCTTAAATATATTGAAAT
AG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(355-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(62-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

55.531

99.779

0.554
  radA Streptococcus mitis NCTC 12261

52.315

95.364

0.499
  radA Streptococcus pneumoniae Rx1

52.315

95.364

0.499
  radA Streptococcus pneumoniae D39

52.315

95.364

0.499
  radA Streptococcus pneumoniae R6

52.315

95.364

0.499
  radA Streptococcus pneumoniae TIGR4

52.315

95.364

0.499
  radA Streptococcus mitis SK321

52.315

95.364

0.499

Multiple sequence alignment