Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AACH41_RS01185 Genome accession   NZ_AP029023
Coordinates   262085..263629 (-) Length   514 a.a.
NCBI ID   WP_275356460.1    Uniprot ID   -
Organism   Methylophilus sp. DW102     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 257085..268629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH41_RS01160 (MTDW_02210) - 257167..257574 (+) 408 WP_194749717.1 polymer-forming cytoskeletal protein -
  AACH41_RS01165 (MTDW_02220) erpA 257641..257997 (+) 357 WP_194749778.1 iron-sulfur cluster insertion protein ErpA -
  AACH41_RS01170 (MTDW_02230) gltA 258076..259365 (-) 1290 WP_194749716.1 citrate synthase -
  AACH41_RS01175 (MTDW_02240) - 259549..260676 (-) 1128 WP_338657565.1 anhydro-N-acetylmuramic acid kinase -
  AACH41_RS01180 (MTDW_02250) tyrS 260801..262039 (+) 1239 WP_194749715.1 tyrosine--tRNA ligase -
  AACH41_RS01185 (MTDW_02260) rpoN/rpoN1 262085..263629 (-) 1545 WP_275356460.1 RNA polymerase factor sigma-54 Machinery gene
  AACH41_RS01190 (MTDW_02270) lptB 263636..264379 (-) 744 WP_194749713.1 LPS export ABC transporter ATP-binding protein -
  AACH41_RS01195 (MTDW_02280) lptA 264388..265002 (-) 615 WP_275356461.1 lipopolysaccharide transport periplasmic protein LptA -
  AACH41_RS01200 (MTDW_02290) lptC 265061..265684 (-) 624 WP_194749711.1 LPS export ABC transporter periplasmic protein LptC -
  AACH41_RS01205 (MTDW_02300) - 265710..266711 (-) 1002 WP_194749710.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 514 a.a.        Molecular weight: 58305.79 Da        Isoelectric Point: 4.5597

>NTDB_id=108156 AACH41_RS01185 WP_275356460.1 262085..263629(-) (rpoN/rpoN1) [Methylophilus sp. DW102]
MKQNLQLRISQNLALTPQLQQSIRLLQLSTLELNQELETILQENPLLEMSDGEEGEFEDNTPPPPTVEAEPTDASSFDMA
TQQEITAPAESLREDLHDELGGSESDLPNLNEEFTPPEFEDDYEEFGSTSNWDEAGRNHQEDEDSDFSRQETTSISLREH
LIDQIQLAHLSQRDMSLVKLLLDSITDDGYLEQDLQEIVEHLPLELEVELLELETALKLIQNLDPVGVGARDLRECLLLQ
LTRLPQETPHLQTAIAIAKDHLALLANKDFVKLRKVLGCDEAVLKAAQHLIRQQNPRPGSEFSSFSHDHFIQHDVVVKKI
KGIWVASLNDGVIPKLRINQMYADILKRNRESSGQYLQSQMQEAKWMIKNIQQRFSTILRVSQAIVDRQRNFFEHGEIAM
RPLVLREIAEELDLHESTVSRVTTHKYMLTPRGVYELKYFFGSSVATDAGGSCSATAIRALIKQMVAEENPKKPLSDNQI
TDTLAQQGIVVARRTIAKYRESLNIPPANLRKTL

Nucleotide


Download         Length: 1545 bp        

>NTDB_id=108156 AACH41_RS01185 WP_275356460.1 262085..263629(-) (rpoN/rpoN1) [Methylophilus sp. DW102]
ATGAAACAGAACCTGCAATTACGTATTTCGCAAAATCTGGCGCTGACGCCGCAGCTGCAGCAATCGATCCGTTTGCTGCA
ACTTTCCACGCTGGAACTGAACCAGGAACTGGAAACCATCCTGCAGGAAAACCCGTTACTGGAAATGTCAGACGGTGAAG
AAGGCGAGTTTGAAGATAACACACCGCCTCCCCCCACTGTAGAAGCTGAGCCGACAGATGCCAGCTCGTTTGACATGGCA
ACGCAGCAAGAGATCACCGCACCGGCAGAAAGTCTGCGTGAAGACTTGCACGATGAGCTGGGCGGGAGCGAGAGCGACCT
GCCCAATTTAAACGAGGAATTCACGCCGCCAGAGTTTGAAGATGATTATGAAGAGTTTGGCAGCACCAGCAACTGGGACG
AAGCCGGACGCAATCATCAGGAAGACGAAGACAGTGACTTTTCCAGACAGGAAACCACCAGCATCAGTTTGCGCGAGCAT
CTGATTGATCAGATACAACTGGCACATTTATCGCAACGTGACATGAGCCTGGTCAAACTGCTACTGGATAGCATCACTGA
TGATGGCTATCTGGAGCAAGATCTGCAGGAGATTGTGGAGCACTTGCCACTCGAACTTGAGGTCGAGCTGCTGGAACTGG
AAACCGCCCTCAAGCTGATCCAGAATCTGGACCCTGTAGGCGTAGGTGCGCGCGATTTACGCGAGTGCCTGCTCTTGCAA
CTGACGCGTTTGCCGCAAGAAACGCCACACTTGCAAACCGCCATTGCCATTGCCAAAGACCACCTGGCGCTGCTGGCCAA
CAAAGACTTTGTCAAACTGCGCAAGGTGCTTGGTTGCGATGAAGCGGTACTCAAGGCGGCGCAGCACCTGATCCGCCAGC
AAAACCCCAGACCCGGCAGCGAGTTTTCCAGCTTTAGTCACGATCACTTTATCCAGCACGATGTGGTGGTTAAAAAAATC
AAGGGCATTTGGGTCGCCTCTTTAAATGATGGCGTGATCCCCAAACTGCGCATCAACCAGATGTATGCGGACATCCTCAA
GCGCAACCGCGAGAGTTCAGGCCAATACCTGCAAAGCCAGATGCAGGAAGCCAAGTGGATGATTAAAAATATCCAGCAGC
GCTTCTCGACCATTTTGCGCGTGTCACAAGCCATTGTAGATCGCCAGCGCAACTTCTTTGAACATGGTGAAATTGCCATG
CGGCCACTGGTGCTGCGTGAAATCGCCGAAGAACTCGACTTGCATGAAAGCACAGTTTCCCGCGTCACCACGCATAAGTA
TATGCTCACACCGCGTGGCGTCTATGAACTCAAATACTTCTTTGGCAGCTCGGTGGCCACAGATGCAGGCGGCTCCTGCT
CTGCCACCGCTATCCGCGCCTTGATCAAGCAAATGGTGGCCGAAGAAAACCCGAAAAAGCCTTTGTCGGACAACCAGATC
ACCGACACACTGGCACAACAAGGTATCGTCGTTGCCCGCCGTACCATAGCCAAATATCGCGAGTCCCTGAATATTCCGCC
CGCGAACTTGCGCAAAACGCTCTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(355-512)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-342)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.136

100

0.525

Multiple sequence alignment