Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   CLC19224_RS06430 Genome accession   NZ_CP176605
Coordinates   1221061..1221975 (-) Length   304 a.a.
NCBI ID   WP_257398898.1    Uniprot ID   -
Organism   Campylobacter lari subsp. concheus strain RM19224     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1215239..1237853 1221061..1221975 within 0


Gene organization within MGE regions


Location: 1215239..1237853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLC19224_RS06400 (CLC19224_01260) - 1215239..1216396 (+) 1158 WP_039668725.1 peptidoglycan DD-metalloendopeptidase family protein -
  CLC19224_RS06405 (CLC19224_01261) mgtE 1216398..1217741 (+) 1344 WP_039668726.1 magnesium transporter -
  CLC19224_RS06410 (CLC19224_01262) tpx 1217754..1218272 (+) 519 WP_039668727.1 thiol peroxidase -
  CLC19224_RS06415 (CLC19224_01263) - 1218269..1219654 (-) 1386 WP_240385536.1 replicative DNA helicase -
  CLC19224_RS06420 (CLC19224_01264) cysK 1219667..1220569 (-) 903 WP_257398897.1 cysteine synthase A -
  CLC19224_RS06425 (CLC19224_01265) - 1220667..1220969 (-) 303 WP_039619003.1 HU family DNA-binding protein -
  CLC19224_RS06430 (CLC19224_01266) waaF 1221061..1221975 (-) 915 WP_257398898.1 lipopolysaccharide heptosyltransferase II Regulator
  CLC19224_RS06435 (CLC19224_01267) - 1222033..1222839 (+) 807 WP_410708269.1 glycosyltransferase family 2 protein -
  CLC19224_RS06440 (CLC19224_01268) - 1222836..1223576 (+) 741 WP_306810895.1 glycosyltransferase family 25 protein -
  CLC19224_RS06445 (CLC19224_01269) - 1223935..1224714 (+) 780 WP_410708363.1 glycosyltransferase family 2 protein -
  CLC19224_RS06450 (CLC19224_01270) - 1224701..1225489 (+) 789 WP_410708270.1 hypothetical protein -
  CLC19224_RS06455 (CLC19224_01271) - 1225486..1226439 (+) 954 WP_410708272.1 glycosyltransferase family 2 protein -
  CLC19224_RS06460 (CLC19224_01272) - 1226436..1227644 (+) 1209 WP_257398808.1 glycosyltransferase family 2 protein -
  CLC19224_RS06465 (CLC19224_01273) - 1227641..1228603 (+) 963 WP_306810885.1 glycosyltransferase family 2 protein -
  CLC19224_RS06470 (CLC19224_01274) - 1228584..1229789 (+) 1206 WP_257398809.1 glycosyltransferase family 8 protein -
  CLC19224_RS06475 (CLC19224_01275) - 1229778..1230833 (-) 1056 WP_257398810.1 glycosyltransferase family 4 protein -
  CLC19224_RS06480 (CLC19224_01276) - 1230830..1232377 (-) 1548 WP_257398811.1 glycosyltransferase family 2 protein -
  CLC19224_RS06485 (CLC19224_01277) - 1232386..1233258 (-) 873 WP_257398812.1 lipid A biosynthesis lauroyl acyltransferase -
  CLC19224_RS06490 (CLC19224_01278) waaC 1233255..1234274 (-) 1020 WP_240386947.1 lipopolysaccharide heptosyltransferase I -
  CLC19224_RS06495 (CLC19224_01279) - 1234341..1235120 (+) 780 WP_257398813.1 3'-5' exonuclease -
  CLC19224_RS06500 (CLC19224_01280) galE 1235179..1236165 (+) 987 WP_039626879.1 UDP-glucose 4-epimerase GalE -
  CLC19224_RS06505 (CLC19224_01281) - 1236159..1237853 (+) 1695 WP_257398814.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 34612.84 Da        Isoelectric Point: 10.3419

>NTDB_id=1081500 CLC19224_RS06430 WP_257398898.1 1221061..1221975(-) (waaF) [Campylobacter lari subsp. concheus strain RM19224]
MNIFINLPTWLGDAVMASAAIYAIKEKYPQAKFTFYGSFVSTELFKHFENAQILVENKKQRYKQILKARKNLGKFDLAFS
FRSAFSSKIILNLIKAKKRFYFDKNILKEEHQVLKYLNFIEKALNFKATSNALKLPIKAKSTQKILGINPGAHFGSAKRW
EASYFARVAKEFSSTHQILIFGVESEREICDEIEQFLLKEGIKAKNLCGKTSIYTLCKNISMLDLLITNDSGPMHIGAVY
GVKTVAIFGSTKFSQTSPWQKNAKIAHLNLACMPCMQKVCPLKHHKCMKDLKPEVVINLAKTFF

Nucleotide


Download         Length: 915 bp        

>NTDB_id=1081500 CLC19224_RS06430 WP_257398898.1 1221061..1221975(-) (waaF) [Campylobacter lari subsp. concheus strain RM19224]
ATGAATATTTTTATCAACCTTCCCACTTGGCTTGGCGATGCGGTGATGGCTAGTGCAGCTATTTATGCTATAAAAGAAAA
ATATCCCCAAGCTAAATTTACTTTTTATGGTTCTTTTGTGAGTACAGAGCTTTTTAAACACTTTGAAAATGCTCAAATTT
TAGTAGAAAACAAAAAACAAAGATATAAGCAAATTTTAAAAGCTAGAAAAAACCTTGGTAAATTTGATTTAGCTTTTTCG
TTTCGCTCGGCATTTTCAAGCAAGATCATTTTAAATCTAATCAAAGCAAAAAAAAGATTTTATTTTGATAAAAATATTCT
AAAAGAAGAACATCAAGTCTTAAAATACTTAAATTTCATAGAAAAAGCCTTAAATTTTAAAGCTACTTCAAATGCTTTAA
AGCTTCCTATAAAAGCAAAATCAACTCAAAAAATTTTAGGTATAAATCCAGGCGCACATTTTGGAAGTGCGAAAAGATGG
GAAGCAAGCTATTTTGCAAGGGTAGCAAAAGAATTTAGCTCCACTCATCAAATTTTGATCTTTGGAGTAGAAAGTGAGAG
AGAAATTTGTGATGAAATTGAACAATTTCTTCTAAAAGAAGGCATAAAAGCAAAAAATCTTTGTGGTAAAACTAGCATTT
ATACTTTATGTAAAAATATTTCTATGCTTGATTTACTCATCACAAATGATAGTGGTCCTATGCATATAGGTGCAGTTTAT
GGAGTGAAAACAGTAGCTATTTTTGGTTCGACTAAATTTAGTCAAACTTCACCTTGGCAAAAAAATGCTAAAATAGCACA
TTTAAATTTAGCTTGTATGCCTTGTATGCAAAAGGTCTGCCCTTTAAAACATCATAAATGTATGAAAGATTTAAAGCCTG
AAGTGGTGATAAATTTAGCAAAAACATTTTTTTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(109-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

65.605

100

0.678

Multiple sequence alignment