Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACH87_RS02720 Genome accession   NZ_AP029019
Coordinates   599900..600943 (-) Length   347 a.a.
NCBI ID   WP_338797185.1    Uniprot ID   -
Organism   Acidovorax sp. DW039     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 594900..605943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH87_RS02695 (ACDW_05320) - 595535..596137 (+) 603 WP_338797180.1 SIS domain-containing protein -
  AACH87_RS02700 (ACDW_05330) - 596134..596793 (+) 660 WP_338797181.1 BON domain-containing protein -
  AACH87_RS02705 (ACDW_05340) - 596897..597811 (-) 915 WP_338797182.1 NAD(P)-dependent oxidoreductase -
  AACH87_RS02710 (ACDW_05350) pilU 597998..599134 (-) 1137 WP_338797183.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AACH87_RS02715 (ACDW_05360) - 599200..599835 (-) 636 WP_338797184.1 cyclic nucleotide-binding domain-containing protein -
  AACH87_RS02720 (ACDW_05370) pilT 599900..600943 (-) 1044 WP_338797185.1 type IV pilus twitching motility protein PilT Machinery gene
  AACH87_RS02725 (ACDW_05380) - 600982..601704 (+) 723 WP_338797187.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH87_RS02730 (ACDW_05390) ltaE 601746..602810 (-) 1065 WP_338797188.1 low-specificity L-threonine aldolase -
  AACH87_RS02735 (ACDW_05400) - 602977..604116 (+) 1140 WP_338797189.1 aminotransferase class V-fold PLP-dependent enzyme -
  AACH87_RS02740 (ACDW_05410) - 604133..605431 (+) 1299 WP_338797190.1 DUF445 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38191.95 Da        Isoelectric Point: 6.7745

>NTDB_id=108113 AACH87_RS02720 WP_338797185.1 599900..600943(-) (pilT) [Acidovorax sp. DW039]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEALDHKTVHAMVYDIMSDSQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLVNQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=108113 AACH87_RS02720 WP_338797185.1 599900..600943(-) (pilT) [Acidovorax sp. DW039]
GTGGACATTACCCAACTGCTGGCATTCAGCGTCAAGAACAAGGCCTCCGACCTGCACTTGTCGGCAGGACTGCCGCCCAT
GATCCGGGTGCATGGCGATGTGCGACGCATCAATGTGGAAGCGCTGGACCACAAGACGGTGCACGCCATGGTGTACGACA
TCATGAGCGACTCGCAGCGCAAACAGTATGAAGAGTTTCTGGAAGTTGACTTCTCGTTCGAGATCGAAGGTCTGGCACGT
TTTCGCGTCAATGCCTTCAACCAGAACCGCGGCGCAGCCGCCGTTTTCCGGACGATTCCGAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCCAAGATTTTTGGCGACCTGGCACTGAAGCCGCGCGGCCTGGTGCTGGTGACAGGCCCTACGGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTCAACTACCTGAACGAAACCGAATACGGCCACATCCTCACGGTGGAAGAT
CCGATCGAGTTCGTGCACGAATCCAAAAAGTGCCTGGTCAACCAGCGTGAAGTCGGCCCGATGACCCTGTCGTTCGCCGC
TGCGCTGAAGTCTGCGCTGCGCGAAGACCCGGACGCCATTCTGGTGGGTGAAATGCGTGACCTCGAAACCATTCGCCTGG
CCATGACGGCTGCGGAAACAGGCCACTTGGTGTTTGGCACGCTGCACACGTCCAGTGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTTCCCGCCGAAGAAAAGGAAATGGTCCGCGCGATGTTGTCTGAGTCGCTGCAAGCGGTGATCTCGCAGAC
ACTGTGCAAGACCAAGGACGGCTCCGGCCGCGTGGCCGCCCATGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTGGGCATGCAGACTCTGGACCAAAAC
CTGACTGATCTGGTCCGCCGCAACATCATCAGCCCTGCAGAGGCACGCAGCAAAGCGAAGATTCCCGAGAACTTCCCAGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

44.776

96.542

0.432
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406

Multiple sequence alignment