Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACK2WG_RS12560 Genome accession   NZ_CP176536
Coordinates   2476691..2477458 (-) Length   255 a.a.
NCBI ID   WP_409508998.1    Uniprot ID   -
Organism   Bacillus spizizenii strain 29-4     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2471691..2482458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2WG_RS12540 (ACK2WG_12540) - 2471865..2472677 (-) 813 WP_079287971.1 pyruvate, water dikinase regulatory protein -
  ACK2WG_RS12545 (ACK2WG_12545) ccpN 2472703..2473341 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  ACK2WG_RS12550 (ACK2WG_12550) glyS 2473474..2475513 (-) 2040 WP_242734014.1 glycine--tRNA ligase subunit beta -
  ACK2WG_RS12555 (ACK2WG_12555) glyQ 2475506..2476393 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  ACK2WG_RS12560 (ACK2WG_12560) recO 2476691..2477458 (-) 768 WP_409508998.1 DNA repair protein RecO Machinery gene
  ACK2WG_RS12565 (ACK2WG_12565) - 2477495..2477638 (-) 144 WP_003230053.1 YqzL family protein -
  ACK2WG_RS12570 (ACK2WG_12570) era 2477782..2478687 (-) 906 WP_014114448.1 GTPase Era -
  ACK2WG_RS12575 (ACK2WG_12575) - 2478668..2479078 (-) 411 WP_003226204.1 cytidine deaminase -
  ACK2WG_RS12580 (ACK2WG_12580) - 2479199..2479570 (-) 372 WP_409508999.1 diacylglycerol kinase family protein -
  ACK2WG_RS12585 (ACK2WG_12585) ybeY 2479551..2480024 (-) 474 WP_014114451.1 rRNA maturation RNase YbeY -
  ACK2WG_RS12590 (ACK2WG_12590) pgpH 2480025..2482160 (-) 2136 WP_072566562.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29313.03 Da        Isoelectric Point: 8.5539

>NTDB_id=1080790 ACK2WG_RS12560 WP_409508998.1 2476691..2477458(-) (recO) [Bacillus spizizenii strain 29-4]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTTGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDKGTEEKKPNPYLFEFILESFKQLNEGTDPDVIIFIAQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRVPIKPQTARLLRLFYYFDLSRLGNVSLKQETKAELKQVIDLYYEEYSGLYLKSKRFL
DQLESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=1080790 ACK2WG_RS12560 WP_409508998.1 2476691..2477458(-) (recO) [Bacillus spizizenii strain 29-4]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTTACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCAAGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCATTTTTAATGCAAAAAACAACGGGCCTCGGAACGCTTCAGCAGGGTGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGATTTGTTTTTAACAGCATATGCTGCTTATATTGCTGAGCTAGTCGACAAAGGCACAGAAGAAAAGAAGCC
AAACCCTTATTTGTTTGAATTCATTTTAGAATCGTTTAAACAGCTGAATGAAGGAACAGATCCGGATGTCATCATCTTCA
TTGCTCAAATGAAGATGCTCGGTGTCATGGGGCTGTACCCTGAGCTGAATCACTGTGTGCATTGTAAAAGCCAGGATGGG
ACCTTCCATTTTTCTGTTCGTGATAACGGTTTTATTTGCCATCGCTGCTTTGAAAAAGATCCCTACAGGGTTCCAATTAA
ACCGCAAACGGCAAGGCTTTTGAGACTGTTTTATTACTTCGATTTGTCGAGGTTGGGCAATGTGTCCTTAAAGCAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTATGAAGAATATTCAGGGTTATATTTAAAATCAAAGCGCTTTTTA
GATCAACTGGAAAGCATGAAACATCTGATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

96.471

100

0.965

Multiple sequence alignment