Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   ACK2WG_RS11030 Genome accession   NZ_CP176536
Coordinates   2206621..2207241 (+) Length   206 a.a.
NCBI ID   WP_014114180.1    Uniprot ID   G4NSU7
Organism   Bacillus spizizenii strain 29-4     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2201621..2212241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2WG_RS10990 (ACK2WG_10990) - 2202761..2203267 (-) 507 WP_061187822.1 PTS glucose transporter subunit IIA -
  ACK2WG_RS10995 (ACK2WG_10995) - 2203405..2203824 (+) 420 WP_079287892.1 Hsp20/alpha crystallin family protein -
  ACK2WG_RS11000 (ACK2WG_11000) - 2203845..2204213 (-) 369 WP_014114177.1 YppG family protein -
  ACK2WG_RS11005 (ACK2WG_11005) - 2204400..2204588 (+) 189 WP_409508956.1 YppF family protein -
  ACK2WG_RS11010 (ACK2WG_11010) - 2204628..2204999 (-) 372 WP_003225610.1 YppE family protein -
  ACK2WG_RS11015 (ACK2WG_11015) - 2205045..2205290 (-) 246 WP_014114178.1 DUF5446 family protein -
  ACK2WG_RS11020 (ACK2WG_11020) sspM 2205489..2205593 (+) 105 WP_003225606.1 acid-soluble spore protein SspM -
  ACK2WG_RS11025 (ACK2WG_11025) - 2205618..2206580 (-) 963 WP_409508957.1 DUF2515 domain-containing protein -
  ACK2WG_RS11030 (ACK2WG_11030) recU 2206621..2207241 (+) 621 WP_014114180.1 Holliday junction resolvase RecU Machinery gene
  ACK2WG_RS11035 (ACK2WG_11035) - 2207263..2210004 (+) 2742 WP_409508958.1 PBP1A family penicillin-binding protein -
  ACK2WG_RS11040 (ACK2WG_11040) - 2210078..2210572 (-) 495 WP_014114182.1 YpoC family protein -
  ACK2WG_RS11045 (ACK2WG_11045) nth 2210569..2211228 (-) 660 WP_003225594.1 endonuclease III -
  ACK2WG_RS11050 (ACK2WG_11050) dnaD 2211247..2211945 (-) 699 WP_072566472.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 23959.09 Da        Isoelectric Point: 9.6510

>NTDB_id=1080778 ACK2WG_RS11030 WP_014114180.1 2206621..2207241(+) (recU) [Bacillus spizizenii strain 29-4]
MIRYPNGKTFQPKHSVSSQNSQKRAPSYSNRGMTLEDDLNETNKYYLTNQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTSFPLQNFHDHQIEHMKQVKAQDGICFVIISAFDQVYFLEADKLFFFWDRKE
KNGRKSIRKDELEESAYPISLGYAPRIDYISIIEQLYFSPSSSAKG

Nucleotide


Download         Length: 621 bp        

>NTDB_id=1080778 ACK2WG_RS11030 WP_014114180.1 2206621..2207241(+) (recU) [Bacillus spizizenii strain 29-4]
GTGATTCGGTATCCTAACGGAAAAACGTTCCAGCCGAAACATTCGGTTTCATCCCAAAACAGTCAGAAAAGGGCCCCGTC
TTACAGTAATCGCGGGATGACCCTCGAAGATGACTTAAACGAAACGAATAAGTATTATCTGACAAACCAAATTGCCGTCA
TACACAAAAAGCCGACACCAGTTCAAATTGTAAATGTCCATTATCCAAAAAGAAGCGCCGCAGTGATTAAAGAAGCTTAC
TTTAAACAATCATCGACAACAGATTATAACGGGATTTACAAAGGGCGGTATATTGATTTTGAGGCGAAAGAAACGAAAAA
CAAGACCTCTTTCCCCCTGCAGAATTTCCATGACCATCAAATCGAGCATATGAAGCAGGTAAAGGCTCAAGACGGTATTT
GTTTTGTTATTATATCCGCTTTCGACCAGGTTTATTTTTTGGAAGCCGATAAGCTGTTTTTTTTCTGGGACAGAAAAGAA
AAAAACGGCAGAAAATCAATTCGAAAAGATGAGTTAGAGGAATCAGCATATCCGATTTCTCTTGGATACGCACCTAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCCATCTTCTAGTGCGAAAGGTTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4NSU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

98.544

100

0.985

Multiple sequence alignment