Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   ACK2WG_RS00020 Genome accession   NZ_CP176536
Coordinates   3030..4142 (+) Length   370 a.a.
NCBI ID   WP_014112268.1    Uniprot ID   G4NT00
Organism   Bacillus spizizenii strain 29-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2WG_RS00005 (ACK2WG_00005) dnaA 1..1341 (+) 1341 WP_003242674.1 chromosomal replication initiator protein DnaA -
  ACK2WG_RS00010 (ACK2WG_00010) dnaN 1531..2667 (+) 1137 WP_003219263.1 DNA polymerase III subunit beta -
  ACK2WG_RS00015 (ACK2WG_00015) rlbA 2799..3014 (+) 216 WP_003219264.1 ribosome maturation protein RlbA -
  ACK2WG_RS00020 (ACK2WG_00020) recF 3030..4142 (+) 1113 WP_014112268.1 DNA replication/repair protein RecF Machinery gene
  ACK2WG_RS00025 (ACK2WG_00025) remB 4160..4405 (+) 246 WP_003219266.1 extracellular matrix regulator RemB -
  ACK2WG_RS00030 (ACK2WG_00030) gyrB 4460..6376 (+) 1917 WP_014112269.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  ACK2WG_RS00035 (ACK2WG_00035) gyrA 6587..9052 (+) 2466 WP_061187096.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42333.38 Da        Isoelectric Point: 7.7139

>NTDB_id=1080742 ACK2WG_RS00020 WP_014112268.1 3030..4142(+) (recF) [Bacillus spizizenii strain 29-4]
MYIQNLELTSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLVEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALEVSDPKDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1080742 ACK2WG_RS00020 WP_014112268.1 3030..4142(+) (recF) [Bacillus spizizenii strain 29-4]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCCCAGGGGAAAACAAACCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACAGGA
CGTCAAATGACAAAGAACTTATACGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCAGTA
TGTCGGGGCCCTCAACACCATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAGGGAAGCCCTCAAGTGAGAAGGCGGT
TTCTTGACATGGAAATCGGACAGGTTTCTCCTGTTTACCTGCATGACCTTTCTCTTTACCAGAAAATCCTTTCGCAGCGA
AATCATTTTTTGAAGCAGCTGCAAACAAGAAAACAAACTGACAGAACGATGCTCGATGTTTTGACCGATCAGCTTGTAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAATTTACAGCGCAGCTCGAAAAATGGGCTCAGCCTATCCATGCGGGCA
TCTCAAGAGGGCTTGAAGAGCTGACCCTGAAGTACCATACGGCTCTTGAGGTATCAGATCCTAAAGACTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGCGGTGTTACGCTGTCAGGGCCTCATCG
TGACGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACGACGGCATTGT
CTCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATACCCCATTTTACTATTGGATGATGTACTGAGT
GAACTGGACGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAATGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4NT00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

99.459

100

0.995

Multiple sequence alignment