Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   ACKW6F_RS10415 Genome accession   NZ_CP176527
Coordinates   2086469..2087104 (+) Length   211 a.a.
NCBI ID   WP_024428112.1    Uniprot ID   A0A5C0WK87
Organism   Bacillus safensis strain D-62     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2081469..2092104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6F_RS10375 (ACKW6F_10375) - 2081618..2082736 (+) 1119 WP_024428115.1 NADH-dependent flavin oxidoreductase -
  ACKW6F_RS10380 (ACKW6F_10380) - 2082791..2083297 (-) 507 WP_024422767.1 PTS glucose transporter subunit IIA -
  ACKW6F_RS10385 (ACKW6F_10385) - 2083467..2083886 (+) 420 WP_056767106.1 Hsp20/alpha crystallin family protein -
  ACKW6F_RS10390 (ACKW6F_10390) - 2083906..2084367 (-) 462 WP_024422769.1 YppG family protein -
  ACKW6F_RS10395 (ACKW6F_10395) - 2084476..2084856 (-) 381 WP_226340828.1 YppE family protein -
  ACKW6F_RS10400 (ACKW6F_10400) - 2084906..2085139 (-) 234 WP_223251001.1 DUF5446 family protein -
  ACKW6F_RS10405 (ACKW6F_10405) sspM 2085337..2085441 (+) 105 WP_024422772.1 acid-soluble spore protein SspM -
  ACKW6F_RS10410 (ACKW6F_10410) - 2085466..2086422 (-) 957 WP_056767111.1 DUF2515 domain-containing protein -
  ACKW6F_RS10415 (ACKW6F_10415) recU 2086469..2087104 (+) 636 WP_024428112.1 Holliday junction resolvase RecU Machinery gene
  ACKW6F_RS10420 (ACKW6F_10420) - 2087124..2089841 (+) 2718 WP_056767113.1 PBP1A family penicillin-binding protein -
  ACKW6F_RS10425 (ACKW6F_10425) - 2089878..2090387 (-) 510 WP_025093074.1 YpoC family protein -
  ACKW6F_RS10430 (ACKW6F_10430) nth 2090384..2091046 (-) 663 WP_024422776.1 endonuclease III -
  ACKW6F_RS10435 (ACKW6F_10435) - 2091064..2091768 (-) 705 WP_024422777.1 DnaD domain-containing protein -

Sequence

Protein


Download         Length: 211 a.a.        Molecular weight: 24317.64 Da        Isoelectric Point: 9.7224

>NTDB_id=1080679 ACKW6F_RS10415 WP_024428112.1 2086469..2087104(+) (recU) [Bacillus safensis strain D-62]
MIRYPNGKSYQPIQQIGTKKRISGESSYSNRGMTLEADLNETNQYYLVNGIAVIHKKPTPVQIVNVDYPKRSAAVIKEAY
FKQSSTTDYNGVYKGRYIDFEAKETKSTTSFPLKNFHDHQIEHMKQVEKQGGICFVIISAFGSVYYLPASDLFYFWERQK
KNGRKSISKDELMEAGHIMTLGYSPRIDYIKVVETLHFSDESNQQLRSKKV

Nucleotide


Download         Length: 636 bp        

>NTDB_id=1080679 ACKW6F_RS10415 WP_024428112.1 2086469..2087104(+) (recU) [Bacillus safensis strain D-62]
ATGATTCGGTATCCAAACGGAAAATCGTATCAGCCCATTCAACAAATTGGGACAAAAAAAAGAATCAGCGGCGAATCCTC
TTATAGTAACCGCGGGATGACGCTTGAGGCTGATTTAAATGAAACGAACCAATACTATTTGGTCAACGGGATCGCAGTCA
TTCATAAAAAGCCTACCCCAGTTCAAATTGTGAATGTGGATTACCCAAAAAGAAGTGCTGCTGTCATTAAAGAAGCCTAT
TTTAAACAATCATCCACAACAGACTATAACGGGGTGTACAAAGGCCGCTATATCGATTTTGAAGCAAAGGAAACCAAAAG
CACTACCTCGTTTCCGCTCAAAAATTTCCACGACCATCAAATTGAGCATATGAAACAGGTTGAAAAGCAAGGCGGCATTT
GTTTCGTTATTATATCCGCTTTTGGCTCCGTTTATTATTTGCCCGCTTCAGATCTCTTTTACTTCTGGGAGCGGCAGAAG
AAAAACGGCCGTAAATCCATTAGCAAAGACGAATTAATGGAAGCTGGCCACATAATGACACTTGGATATTCGCCAAGAAT
TGATTATATTAAAGTAGTGGAGACACTTCATTTTTCGGATGAAAGCAATCAGCAATTACGTTCGAAAAAGGTTTAA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WK87

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

73.762

95.735

0.706

Multiple sequence alignment