Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACKW6F_RS08895 Genome accession   NZ_CP176527
Coordinates   1784055..1784435 (+) Length   126 a.a.
NCBI ID   WP_065214465.1    Uniprot ID   -
Organism   Bacillus safensis strain D-62     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1779055..1789435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6F_RS08885 (ACKW6F_08885) - 1779552..1782695 (+) 3144 WP_149126620.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  ACKW6F_RS08890 (ACKW6F_08890) - 1782889..1783797 (+) 909 WP_024425124.1 trypsin-like serine peptidase -
  ACKW6F_RS08895 (ACKW6F_08895) nucA/comI 1784055..1784435 (+) 381 WP_065214465.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACKW6F_RS08900 (ACKW6F_08900) - 1784581..1785429 (+) 849 WP_024427077.1 STAS domain-containing protein -
  ACKW6F_RS08905 (ACKW6F_08905) - 1785462..1786004 (-) 543 WP_024425127.1 IseA DL-endopeptidase inhibitor family protein -
  ACKW6F_RS08910 (ACKW6F_08910) - 1786301..1786747 (+) 447 WP_149126619.1 hypothetical protein -
  ACKW6F_RS08915 (ACKW6F_08915) lexA 1786793..1787413 (-) 621 WP_024425129.1 transcriptional repressor LexA -
  ACKW6F_RS08920 (ACKW6F_08920) yneA 1787572..1787883 (+) 312 WP_024425130.1 cell division suppressor protein YneA -
  ACKW6F_RS08925 (ACKW6F_08925) - 1787901..1788548 (+) 648 WP_025093247.1 recombinase family protein -
  ACKW6F_RS08930 (ACKW6F_08930) - 1788618..1788848 (+) 231 WP_024425132.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13833.49 Da        Isoelectric Point: 7.9051

>NTDB_id=1080678 ACKW6F_RS08895 WP_065214465.1 1784055..1784435(+) (nucA/comI) [Bacillus safensis strain D-62]
MGTLFGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISEFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=1080678 ACKW6F_RS08895 WP_065214465.1 1784055..1784435(+) (nucA/comI) [Bacillus safensis strain D-62]
ATGGGCACGTTGTTTGGCGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGGGCAGGCTCATG
GGTCGGGCATCAAATCAGCGAGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment