Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   ACKW6F_RS08020 Genome accession   NZ_CP176527
Coordinates   1600179..1601081 (+) Length   300 a.a.
NCBI ID   WP_025092576.1    Uniprot ID   A0A5C0WNT3
Organism   Bacillus safensis strain D-62     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1595179..1606081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6F_RS08000 (ACKW6F_08000) - 1595845..1597581 (+) 1737 WP_149126668.1 glycosyltransferase -
  ACKW6F_RS08005 (ACKW6F_08005) - 1597578..1597853 (+) 276 WP_149126667.1 FlhB-like flagellar biosynthesis protein -
  ACKW6F_RS08010 (ACKW6F_08010) sucC 1598030..1599190 (+) 1161 WP_024426972.1 ADP-forming succinate--CoA ligase subunit beta -
  ACKW6F_RS08015 (ACKW6F_08015) sucD 1599213..1600115 (+) 903 WP_007499624.1 succinate--CoA ligase subunit alpha -
  ACKW6F_RS08020 (ACKW6F_08020) dprA 1600179..1601081 (+) 903 WP_025092576.1 DNA-processing protein DprA Machinery gene
  ACKW6F_RS08025 (ACKW6F_08025) topA 1601275..1603350 (+) 2076 WP_024424184.1 type I DNA topoisomerase -
  ACKW6F_RS08030 (ACKW6F_08030) trmFO 1603421..1604725 (+) 1305 WP_149126666.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  ACKW6F_RS08035 (ACKW6F_08035) xerC 1604788..1605705 (+) 918 WP_024424182.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34070.43 Da        Isoelectric Point: 8.8768

>NTDB_id=1080674 ACKW6F_RS08020 WP_025092576.1 1600179..1601081(+) (dprA) [Bacillus safensis strain D-62]
MYNVSERMIFHRLKGLISPSLLTKWWKVDPELYINEETHHFKQDRALQTIDFTRLKQAEENEFPIFQHIVQAYLKQNIHM
IPITSPLYPSTLKNIYDPPPVLFLKGNVSYLNEEKSLGVVGTRVPSSYGEACVKKIVGELVKEEWMIVSGLAKGIDGLAH
KECIRKKGKTIGIIAGGFQHVYPKEHAQMAQYMGEHHLLLSEHPPYVKPEKWHFPMRNRLISALTKGTIVIQCKEKSGSL
ITAYQALEQGKEVFAVAGSIFDSNSTGPARLIQQGAKLVHSTKDILEEFSFHSVQYTELS

Nucleotide


Download         Length: 903 bp        

>NTDB_id=1080674 ACKW6F_RS08020 WP_025092576.1 1600179..1601081(+) (dprA) [Bacillus safensis strain D-62]
ATGTACAACGTGTCCGAAAGAATGATTTTTCACCGCTTAAAAGGCCTCATCTCACCCTCATTATTAACAAAATGGTGGAA
AGTCGATCCTGAACTATATATAAATGAAGAAACACATCATTTTAAACAGGATCGAGCATTACAAACGATCGACTTTACCC
GCTTAAAACAAGCTGAAGAAAATGAATTCCCCATTTTTCAACACATCGTTCAAGCCTATTTAAAGCAAAACATTCACATG
ATTCCCATCACATCACCCTTATATCCCAGCACACTAAAAAATATTTATGATCCTCCCCCTGTGTTATTCCTAAAAGGAAA
CGTATCATATTTAAATGAAGAAAAAAGTTTAGGTGTAGTCGGCACGCGAGTTCCATCGTCTTATGGAGAAGCATGTGTGA
AGAAAATTGTTGGTGAGCTTGTCAAGGAAGAGTGGATGATTGTCAGTGGTTTAGCTAAAGGAATCGATGGACTGGCACAT
AAAGAGTGCATCAGGAAAAAAGGGAAAACAATCGGCATTATAGCAGGCGGCTTTCAGCATGTATACCCAAAAGAACATGC
GCAAATGGCTCAATACATGGGTGAACATCATTTGCTTTTGTCAGAGCACCCGCCTTATGTCAAACCAGAAAAGTGGCATT
TTCCAATGAGAAATCGTTTAATTAGTGCATTGACGAAAGGGACCATCGTGATTCAGTGCAAAGAAAAGAGCGGTTCTCTT
ATTACAGCCTATCAAGCACTTGAGCAAGGCAAAGAAGTATTTGCGGTTGCTGGATCCATCTTTGATTCTAATTCCACAGG
TCCGGCAAGACTCATTCAGCAGGGGGCAAAGCTTGTTCATTCCACAAAGGATATTTTAGAGGAATTCTCCTTCCACAGCG
TTCAATATACTGAACTCTCATAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WNT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

54.355

95.667

0.52
  dprA Legionella pneumophila strain ERS1305867

37.705

100

0.383

Multiple sequence alignment