Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACKW6F_RS01915 Genome accession   NZ_CP176527
Coordinates   357304..357732 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus safensis strain D-62     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 352304..362732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6F_RS01885 (ACKW6F_01885) - 352467..352796 (+) 330 WP_024424311.1 YckD family protein -
  ACKW6F_RS01890 (ACKW6F_01890) - 352840..353604 (-) 765 WP_149126012.1 MBL fold metallo-hydrolase -
  ACKW6F_RS01895 (ACKW6F_01895) - 353720..354136 (+) 417 WP_025092811.1 Lrp/AsnC family transcriptional regulator -
  ACKW6F_RS01900 (ACKW6F_01900) - 354165..354971 (+) 807 WP_149126011.1 aminoglycoside N(3)-acetyltransferase -
  ACKW6F_RS01905 (ACKW6F_01905) - 354973..355359 (-) 387 WP_149126010.1 competence protein ComJ -
  ACKW6F_RS01910 (ACKW6F_01910) - 355479..357176 (-) 1698 WP_056765079.1 methyl-accepting chemotaxis protein -
  ACKW6F_RS01915 (ACKW6F_01915) nucA/comI 357304..357732 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACKW6F_RS01920 (ACKW6F_01920) - 358100..360169 (+) 2070 WP_149126009.1 hydantoinase/oxoprolinase family protein -
  ACKW6F_RS01925 (ACKW6F_01925) - 360159..362147 (+) 1989 WP_122631501.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=1080653 ACKW6F_RS01915 WP_024424305.1 357304..357732(-) (nucA/comI) [Bacillus safensis strain D-62]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1080653 ACKW6F_RS01915 WP_024424305.1 357304..357732(-) (nucA/comI) [Bacillus safensis strain D-62]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCATCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCAATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATTCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535

Multiple sequence alignment