Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AACJ17_RS04670 Genome accession   NZ_AP029010
Coordinates   973210..974709 (-) Length   499 a.a.
NCBI ID   WP_020831172.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain MAFF 301524     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 968210..979709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACJ17_RS04640 (MAFF301524_09060) - 968717..969367 (-) 651 WP_016724261.1 LON peptidase substrate-binding domain-containing protein -
  AACJ17_RS04645 (MAFF301524_09070) rapZ 969445..970335 (-) 891 WP_011000365.1 RNase adapter RapZ -
  AACJ17_RS04650 (MAFF301524_09080) - 970429..970764 (-) 336 WP_020425146.1 PsiF family protein -
  AACJ17_RS04655 (MAFF301524_09090) hprK 970955..971929 (-) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  AACJ17_RS04660 (MAFF301524_09100) ptsN 972000..972455 (-) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AACJ17_RS04665 (MAFF301524_09110) raiA 972805..973158 (-) 354 WP_011000368.1 ribosome-associated translation inhibitor RaiA -
  AACJ17_RS04670 (MAFF301524_09120) rpoN/rpoN1 973210..974709 (-) 1500 WP_020831172.1 RNA polymerase factor sigma-54 Machinery gene
  AACJ17_RS04675 (MAFF301524_09130) lptB 974807..975595 (-) 789 WP_016724264.1 LPS export ABC transporter ATP-binding protein -
  AACJ17_RS04680 (MAFF301524_09140) lptA 975629..976204 (-) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  AACJ17_RS04685 (MAFF301524_09150) lptC 976273..976905 (-) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  AACJ17_RS04690 (MAFF301524_09160) - 976907..977494 (-) 588 WP_011000373.1 HAD family hydrolase -
  AACJ17_RS04695 (MAFF301524_09170) - 977533..978516 (-) 984 WP_016724265.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55213.53 Da        Isoelectric Point: 4.8037

>NTDB_id=108020 AACJ17_RS04670 WP_020831172.1 973210..974709(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain MAFF 301524]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTADASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=108020 AACJ17_RS04670 WP_020831172.1 973210..974709(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain MAFF 301524]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGTGCGGACCATGCCGACACAGCCGACGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

99.8

100

0.998

Multiple sequence alignment