Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACKWCM_RS12020 Genome accession   NZ_CP176416
Coordinates   2370522..2371292 (-) Length   256 a.a.
NCBI ID   WP_197172661.1    Uniprot ID   -
Organism   Bacillus safensis strain GUCCb3-7     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2365522..2376292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWCM_RS12000 (ACKWCM_12000) - 2365614..2366435 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  ACKWCM_RS12005 (ACKWCM_12005) - 2366461..2367099 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  ACKWCM_RS12010 (ACKWCM_12010) glyS 2367267..2369312 (-) 2046 WP_024423018.1 glycine--tRNA ligase subunit beta -
  ACKWCM_RS12015 (ACKWCM_12015) glyQ 2369305..2370192 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  ACKWCM_RS12020 (ACKWCM_12020) recO 2370522..2371292 (-) 771 WP_197172661.1 DNA repair protein RecO Machinery gene
  ACKWCM_RS12025 (ACKWCM_12025) - 2371328..2371471 (-) 144 WP_003217288.1 YqzL family protein -
  ACKWCM_RS12030 (ACKWCM_12030) era 2371620..2372525 (-) 906 WP_007501305.1 GTPase Era -
  ACKWCM_RS12035 (ACKWCM_12035) - 2372518..2372916 (-) 399 WP_008356263.1 cytidine deaminase -
  ACKWCM_RS12040 (ACKWCM_12040) - 2373030..2373416 (-) 387 WP_024423020.1 diacylglycerol kinase family protein -
  ACKWCM_RS12045 (ACKWCM_12045) ybeY 2373394..2373873 (-) 480 WP_024423021.1 rRNA maturation RNase YbeY -
  ACKWCM_RS12050 (ACKWCM_12050) - 2373874..2375985 (-) 2112 WP_370880885.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29337.98 Da        Isoelectric Point: 8.1204

>NTDB_id=1079977 ACKWCM_RS12020 WP_197172661.1 2370522..2371292(-) (recO) [Bacillus safensis strain GUCCb3-7]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRA
IREDLFLTAYAAYMTELMDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKQQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMSDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=1079977 ACKWCM_RS12020 WP_197172661.1 2370522..2371292(-) (recO) [Bacillus safensis strain GUCCb3-7]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGAAAAATTGGTGTGATGGCCAGAGGCGCTAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGCACCTTTCTCATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGGCC
ATTCGGGAAGACTTATTTCTGACCGCATATGCCGCATATATGACGGAATTAATGGATAAAGGGACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAACGAAGGCACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTTGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCGATAAGAGATAACGGCTTCATTTGCCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCCGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTTGATGTGAAACAGCAGA
CGAAACAAGAAATTCGTCAAGTGCTTGATCACTACTATGATGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGAGTGACGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75

100

0.75

Multiple sequence alignment