Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | ACKWCM_RS08945 | Genome accession | NZ_CP176416 |
| Coordinates | 1790200..1790580 (+) | Length | 126 a.a. |
| NCBI ID | WP_065214465.1 | Uniprot ID | - |
| Organism | Bacillus safensis strain GUCCb3-7 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 1745510..1794993 | 1790200..1790580 | within | 0 |
Gene organization within MGE regions
Location: 1745510..1794993
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACKWCM_RS08675 (ACKWCM_08675) | - | 1745510..1745812 (-) | 303 | WP_311165352.1 | tyrosine-type recombinase/integrase | - |
| ACKWCM_RS08680 (ACKWCM_08680) | - | 1745869..1746063 (+) | 195 | WP_373393563.1 | XtrA/YqaO family protein | - |
| ACKWCM_RS08685 (ACKWCM_08685) | - | 1746236..1747303 (+) | 1068 | WP_311165355.1 | patatin-like phospholipase family protein | - |
| ACKWCM_RS08690 (ACKWCM_08690) | - | 1747607..1747753 (+) | 147 | WP_311165356.1 | hypothetical protein | - |
| ACKWCM_RS08695 (ACKWCM_08695) | - | 1747820..1748212 (+) | 393 | WP_311165357.1 | DNA methyltransferase | - |
| ACKWCM_RS08700 (ACKWCM_08700) | - | 1748169..1748387 (+) | 219 | WP_311165358.1 | site-specific DNA-methyltransferase | - |
| ACKWCM_RS08705 (ACKWCM_08705) | - | 1748507..1748845 (+) | 339 | WP_311165359.1 | hypothetical protein | - |
| ACKWCM_RS08710 (ACKWCM_08710) | - | 1748937..1749059 (+) | 123 | WP_311165360.1 | hypothetical protein | - |
| ACKWCM_RS08715 (ACKWCM_08715) | - | 1749075..1749539 (+) | 465 | WP_311165361.1 | hypothetical protein | - |
| ACKWCM_RS08720 (ACKWCM_08720) | - | 1749695..1750195 (+) | 501 | WP_311165362.1 | hypothetical protein | - |
| ACKWCM_RS08725 (ACKWCM_08725) | - | 1750267..1750830 (+) | 564 | WP_311165363.1 | hypothetical protein | - |
| ACKWCM_RS08730 (ACKWCM_08730) | terS | 1750984..1751701 (+) | 718 | Protein_1671 | phage terminase small subunit | - |
| ACKWCM_RS08735 (ACKWCM_08735) | - | 1751701..1752273 (+) | 573 | WP_396133867.1 | PBSX family phage terminase large subunit | - |
| ACKWCM_RS08740 (ACKWCM_08740) | - | 1752281..1752505 (+) | 225 | WP_396133868.1 | phage terminase large subunit | - |
| ACKWCM_RS08745 (ACKWCM_08745) | - | 1752510..1752846 (+) | 337 | Protein_1674 | terminase large subunit | - |
| ACKWCM_RS08750 (ACKWCM_08750) | - | 1752922..1754048 (+) | 1127 | Protein_1675 | phage portal protein | - |
| ACKWCM_RS08755 (ACKWCM_08755) | - | 1754019..1754495 (-) | 477 | WP_373393560.1 | phage tail assembly chaperone | - |
| ACKWCM_RS08760 (ACKWCM_08760) | - | 1754891..1755184 (-) | 294 | Protein_1677 | phage tail tube protein | - |
| ACKWCM_RS08765 (ACKWCM_08765) | - | 1755176..1755691 (+) | 516 | Protein_1678 | peptidoglycan-binding protein | - |
| ACKWCM_RS08770 (ACKWCM_08770) | - | 1755813..1756133 (-) | 321 | WP_311165364.1 | hypothetical protein | - |
| ACKWCM_RS08775 (ACKWCM_08775) | - | 1756276..1758081 (+) | 1806 | WP_311165365.1 | S8 family peptidase | - |
| ACKWCM_RS08780 (ACKWCM_08780) | - | 1758258..1759208 (+) | 951 | WP_311165366.1 | S8 family peptidase | - |
| ACKWCM_RS08785 (ACKWCM_08785) | - | 1759495..1759647 (+) | 153 | WP_311165367.1 | hypothetical protein | - |
| ACKWCM_RS08790 (ACKWCM_08790) | - | 1759678..1759914 (+) | 237 | WP_311165368.1 | hypothetical protein | - |
| ACKWCM_RS08795 (ACKWCM_08795) | - | 1759886..1760287 (+) | 402 | WP_311165369.1 | hypothetical protein | - |
| ACKWCM_RS08800 (ACKWCM_08800) | - | 1760253..1760879 (+) | 627 | WP_311165370.1 | hypothetical protein | - |
| ACKWCM_RS08805 (ACKWCM_08805) | - | 1760962..1761150 (-) | 189 | WP_311165371.1 | BlaI/MecI/CopY family transcriptional regulator | - |
| ACKWCM_RS08810 (ACKWCM_08810) | - | 1761231..1762067 (+) | 837 | WP_311165372.1 | SMI1/KNR4 family protein | - |
| ACKWCM_RS08815 (ACKWCM_08815) | - | 1762270..1763376 (+) | 1107 | WP_167753078.1 | Rap family tetratricopeptide repeat protein | - |
| ACKWCM_RS08820 (ACKWCM_08820) | - | 1764819..1765003 (+) | 185 | Protein_1689 | nuclease | - |
| ACKWCM_RS08825 (ACKWCM_08825) | - | 1765169..1765558 (+) | 390 | WP_311165373.1 | hypothetical protein | - |
| ACKWCM_RS08830 (ACKWCM_08830) | - | 1765839..1766642 (+) | 804 | WP_311165374.1 | hypothetical protein | - |
| ACKWCM_RS08835 (ACKWCM_08835) | - | 1767425..1767559 (+) | 135 | WP_254966822.1 | hypothetical protein | - |
| ACKWCM_RS08840 (ACKWCM_08840) | - | 1767649..1769577 (+) | 1929 | WP_341320049.1 | T7SS effector LXG polymorphic toxin | - |
| ACKWCM_RS08845 (ACKWCM_08845) | - | 1769585..1770067 (+) | 483 | WP_341320048.1 | immunity protein YezG family protein | - |
| ACKWCM_RS08850 (ACKWCM_08850) | - | 1770155..1770616 (-) | 462 | WP_044335609.1 | macro domain-containing protein | - |
| ACKWCM_RS08855 (ACKWCM_08855) | - | 1770844..1771425 (+) | 582 | WP_034621968.1 | undecaprenyl-diphosphatase | - |
| ACKWCM_RS08860 (ACKWCM_08860) | - | 1771477..1771848 (-) | 372 | WP_056766571.1 | iron chaperone | - |
| ACKWCM_RS08865 (ACKWCM_08865) | - | 1772391..1773503 (+) | 1113 | WP_024425110.1 | tetratricopeptide repeat protein | - |
| ACKWCM_RS08870 (ACKWCM_08870) | - | 1773959..1774192 (+) | 234 | WP_024425111.1 | hypothetical protein | - |
| ACKWCM_RS08875 (ACKWCM_08875) | - | 1774233..1774928 (-) | 696 | WP_024427061.1 | YoaK family protein | - |
| ACKWCM_RS08880 (ACKWCM_08880) | - | 1774996..1776828 (-) | 1833 | WP_311165376.1 | DNA ligase D | - |
| ACKWCM_RS08885 (ACKWCM_08885) | - | 1776944..1777792 (+) | 849 | WP_024425113.1 | Ku protein | - |
| ACKWCM_RS08890 (ACKWCM_08890) | - | 1778003..1778446 (-) | 444 | WP_311165377.1 | DUF2188 domain-containing protein | - |
| ACKWCM_RS08895 (ACKWCM_08895) | - | 1778612..1779085 (+) | 474 | WP_311165378.1 | VOC family protein | - |
| ACKWCM_RS08900 (ACKWCM_08900) | - | 1779134..1779547 (+) | 414 | WP_025093258.1 | Rrf2 family transcriptional regulator | - |
| ACKWCM_RS08905 (ACKWCM_08905) | - | 1779649..1780500 (+) | 852 | WP_098676909.1 | SDR family oxidoreductase | - |
| ACKWCM_RS08910 (ACKWCM_08910) | - | 1780655..1781563 (+) | 909 | WP_311165379.1 | DMT family transporter | - |
| ACKWCM_RS08915 (ACKWCM_08915) | - | 1781576..1781767 (-) | 192 | WP_025093255.1 | hypothetical protein | - |
| ACKWCM_RS08920 (ACKWCM_08920) | - | 1781878..1784094 (+) | 2217 | WP_311165380.1 | RNA ligase | - |
| ACKWCM_RS08925 (ACKWCM_08925) | - | 1784159..1784869 (+) | 711 | WP_025093253.1 | DUF421 domain-containing protein | - |
| ACKWCM_RS08930 (ACKWCM_08930) | - | 1785097..1785675 (+) | 579 | WP_024427074.1 | TetR/AcrR family transcriptional regulator | - |
| ACKWCM_RS08935 (ACKWCM_08935) | - | 1785697..1788840 (+) | 3144 | WP_197172870.1 | bifunctional cytochrome P450/NADPH--P450 reductase | - |
| ACKWCM_RS08940 (ACKWCM_08940) | - | 1789034..1789942 (+) | 909 | WP_024425124.1 | serine protease | - |
| ACKWCM_RS08945 (ACKWCM_08945) | nucA/comI | 1790200..1790580 (+) | 381 | WP_065214465.1 | NucA/NucB deoxyribonuclease domain-containing protein | Machinery gene |
| ACKWCM_RS08950 (ACKWCM_08950) | - | 1790726..1791574 (+) | 849 | WP_024427077.1 | STAS domain-containing protein | - |
| ACKWCM_RS08955 (ACKWCM_08955) | - | 1791607..1792149 (-) | 543 | WP_024425127.1 | IseA DL-endopeptidase inhibitor family protein | - |
| ACKWCM_RS08960 (ACKWCM_08960) | - | 1792446..1792892 (+) | 447 | WP_149126619.1 | hypothetical protein | - |
| ACKWCM_RS08965 (ACKWCM_08965) | lexA | 1792938..1793558 (-) | 621 | WP_024425129.1 | transcriptional repressor LexA | - |
| ACKWCM_RS08970 (ACKWCM_08970) | yneA | 1793717..1794028 (+) | 312 | WP_024425130.1 | cell division suppressor protein YneA | - |
| ACKWCM_RS08975 (ACKWCM_08975) | - | 1794046..1794693 (+) | 648 | WP_025093247.1 | recombinase family protein | - |
| ACKWCM_RS08980 (ACKWCM_08980) | - | 1794763..1794993 (+) | 231 | WP_024425132.1 | DUF896 domain-containing protein | - |
Sequence
Protein
Download Length: 126 a.a. Molecular weight: 13833.49 Da Isoelectric Point: 7.9051
>NTDB_id=1079970 ACKWCM_RS08945 WP_065214465.1 1790200..1790580(+) (nucA/comI) [Bacillus safensis strain GUCCb3-7]
MGTLFGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISEFPDGKRILFIVK
MGTLFGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISEFPDGKRILFIVK
Nucleotide
Download Length: 381 bp
>NTDB_id=1079970 ACKWCM_RS08945 WP_065214465.1 1790200..1790580(+) (nucA/comI) [Bacillus safensis strain GUCCb3-7]
ATGGGCACGTTGTTTGGCGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGGGCAGGCTCATG
GGTCGGGCATCAAATCAGCGAGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA
ATGGGCACGTTGTTTGGCGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGGGCAGGCTCATG
GGTCGGGCATCAAATCAGCGAGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
57.983 |
94.444 |
0.548 |