Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   ACKWCM_RS07950 Genome accession   NZ_CP176416
Coordinates   1595118..1596020 (+) Length   300 a.a.
NCBI ID   WP_025092576.1    Uniprot ID   A0A5C0WNT3
Organism   Bacillus safensis strain GUCCb3-7     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1590118..1601020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWCM_RS07930 (ACKWCM_07930) - 1590781..1592520 (+) 1740 WP_185854402.1 glycosyltransferase -
  ACKWCM_RS07935 (ACKWCM_07935) - 1592517..1592792 (+) 276 WP_149126667.1 FlhB-like flagellar biosynthesis protein -
  ACKWCM_RS07940 (ACKWCM_07940) sucC 1592969..1594129 (+) 1161 WP_024426972.1 ADP-forming succinate--CoA ligase subunit beta -
  ACKWCM_RS07945 (ACKWCM_07945) sucD 1594152..1595054 (+) 903 WP_007499624.1 succinate--CoA ligase subunit alpha -
  ACKWCM_RS07950 (ACKWCM_07950) dprA 1595118..1596020 (+) 903 WP_025092576.1 DNA-processing protein DprA Machinery gene
  ACKWCM_RS07955 (ACKWCM_07955) topA 1596214..1598289 (+) 2076 WP_024424184.1 type I DNA topoisomerase -
  ACKWCM_RS07960 (ACKWCM_07960) trmFO 1598360..1599664 (+) 1305 WP_149126666.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  ACKWCM_RS07965 (ACKWCM_07965) xerC 1599727..1600644 (+) 918 WP_024424182.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34070.43 Da        Isoelectric Point: 8.8768

>NTDB_id=1079966 ACKWCM_RS07950 WP_025092576.1 1595118..1596020(+) (dprA) [Bacillus safensis strain GUCCb3-7]
MYNVSERMIFHRLKGLISPSLLTKWWKVDPELYINEETHHFKQDRALQTIDFTRLKQAEENEFPIFQHIVQAYLKQNIHM
IPITSPLYPSTLKNIYDPPPVLFLKGNVSYLNEEKSLGVVGTRVPSSYGEACVKKIVGELVKEEWMIVSGLAKGIDGLAH
KECIRKKGKTIGIIAGGFQHVYPKEHAQMAQYMGEHHLLLSEHPPYVKPEKWHFPMRNRLISALTKGTIVIQCKEKSGSL
ITAYQALEQGKEVFAVAGSIFDSNSTGPARLIQQGAKLVHSTKDILEEFSFHSVQYTELS

Nucleotide


Download         Length: 903 bp        

>NTDB_id=1079966 ACKWCM_RS07950 WP_025092576.1 1595118..1596020(+) (dprA) [Bacillus safensis strain GUCCb3-7]
ATGTACAACGTGTCCGAAAGAATGATTTTTCACCGCTTAAAAGGCCTCATCTCACCCTCATTATTAACAAAATGGTGGAA
AGTCGATCCTGAACTATATATAAATGAAGAAACACATCATTTTAAACAGGATCGAGCATTACAAACGATCGACTTTACCC
GCTTAAAACAAGCTGAAGAAAATGAATTCCCCATTTTTCAACACATCGTTCAAGCCTATTTAAAGCAAAACATTCACATG
ATTCCCATCACATCACCCTTATATCCCAGCACACTAAAAAATATTTATGATCCTCCCCCTGTGTTATTCCTAAAAGGAAA
CGTATCATATTTAAATGAAGAAAAAAGTTTAGGTGTAGTCGGCACGCGAGTTCCATCGTCTTATGGAGAAGCATGTGTGA
AGAAAATTGTTGGTGAGCTTGTCAAGGAAGAGTGGATGATTGTCAGTGGTTTAGCTAAAGGAATCGATGGACTGGCACAT
AAAGAGTGCATCAGGAAAAAAGGGAAAACAATCGGCATTATAGCAGGCGGCTTTCAGCATGTATACCCAAAAGAACATGC
GCAAATGGCTCAATACATGGGTGAACATCATTTGCTTTTGTCAGAGCACCCGCCTTATGTCAAACCAGAAAAGTGGCATT
TTCCAATGAGAAATCGTTTAATTAGTGCATTGACGAAAGGGACCATCGTGATTCAGTGCAAAGAAAAGAGCGGTTCTCTT
ATTACAGCCTATCAAGCACTTGAGCAAGGCAAAGAAGTATTTGCGGTTGCTGGATCCATCTTTGATTCTAATTCCACAGG
TCCGGCAAGACTCATTCAGCAGGGGGCAAAGCTTGTTCATTCCACAAAGGATATTTTAGAGGAATTCTCCTTCCACAGCG
TTCAATATACTGAACTCTCATAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WNT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

54.355

95.667

0.52
  dprA Legionella pneumophila strain ERS1305867

37.705

100

0.383

Multiple sequence alignment