Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACKPXQ_RS12140 Genome accession   NZ_CP176038
Coordinates   2521453..2522220 (-) Length   255 a.a.
NCBI ID   WP_007408287.1    Uniprot ID   A0AAP3YCF1
Organism   Bacillus velezensis strain A4     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2516453..2527220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKPXQ_RS12120 (ACKPXQ_12120) - 2516655..2517467 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  ACKPXQ_RS12125 (ACKPXQ_12125) ccpN 2517493..2518131 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  ACKPXQ_RS12130 (ACKPXQ_12130) glyS 2518232..2520271 (-) 2040 WP_128496724.1 glycine--tRNA ligase subunit beta -
  ACKPXQ_RS12135 (ACKPXQ_12135) glyQ 2520264..2521151 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  ACKPXQ_RS12140 (ACKPXQ_12140) recO 2521453..2522220 (-) 768 WP_007408287.1 DNA repair protein RecO Machinery gene
  ACKPXQ_RS12145 (ACKPXQ_12145) - 2522257..2522400 (-) 144 WP_007408286.1 YqzL family protein -
  ACKPXQ_RS12150 (ACKPXQ_12150) era 2522556..2523461 (-) 906 WP_007408285.1 GTPase Era -
  ACKPXQ_RS12155 (ACKPXQ_12155) - 2523442..2523852 (-) 411 WP_003152976.1 cytidine deaminase -
  ACKPXQ_RS12160 (ACKPXQ_12160) - 2523976..2524350 (-) 375 WP_003152974.1 diacylglycerol kinase family protein -
  ACKPXQ_RS12165 (ACKPXQ_12165) ybeY 2524331..2524804 (-) 474 WP_053284930.1 rRNA maturation RNase YbeY -
  ACKPXQ_RS12170 (ACKPXQ_12170) - 2524805..2526937 (-) 2133 WP_014418406.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29292.91 Da        Isoelectric Point: 8.5625

>NTDB_id=1079523 ACKPXQ_RS12140 WP_007408287.1 2521453..2522220(-) (recO) [Bacillus velezensis strain A4]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=1079523 ACKPXQ_RS12140 WP_007408287.1 2521453..2522220(-) (recO) [Bacillus velezensis strain A4]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATACTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.02

100

0.89

Multiple sequence alignment