Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AACK42_RS01730 Genome accession   NZ_AP028996
Coordinates   381500..383020 (-) Length   506 a.a.
NCBI ID   WP_180721445.1    Uniprot ID   -
Organism   Paraburkholderia sp. 22B1P     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 376500..388020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACK42_RS01705 (PBP221_03350) - 377276..377911 (-) 636 WP_180721444.1 LON peptidase substrate-binding domain-containing protein -
  AACK42_RS01710 (PBP221_03360) rapZ 377976..378869 (-) 894 WP_054929656.1 RNase adapter RapZ -
  AACK42_RS01715 (PBP221_03370) hprK 378947..379915 (-) 969 WP_007579699.1 HPr(Ser) kinase/phosphatase -
  AACK42_RS01720 (PBP221_03380) - 380148..380663 (-) 516 WP_028365018.1 PTS sugar transporter subunit IIA -
  AACK42_RS01725 (PBP221_03390) raiA 380965..381324 (-) 360 WP_012399744.1 ribosome-associated translation inhibitor RaiA -
  AACK42_RS01730 (PBP221_03400) rpoN/rpoN1 381500..383020 (-) 1521 WP_180721445.1 RNA polymerase factor sigma-54 Machinery gene
  AACK42_RS01735 (PBP221_03410) lptB 383253..384035 (-) 783 WP_007579703.1 LPS export ABC transporter ATP-binding protein -
  AACK42_RS01740 (PBP221_03420) lptA 384032..384709 (-) 678 WP_180721446.1 lipopolysaccharide transport periplasmic protein LptA -
  AACK42_RS01745 (PBP221_03430) lptC 384731..385336 (-) 606 WP_180721447.1 LPS export ABC transporter periplasmic protein LptC -
  AACK42_RS01750 (PBP221_03440) - 385333..385893 (-) 561 WP_238266525.1 HAD family hydrolase -
  AACK42_RS01755 (PBP221_03450) kdsD 385893..386876 (-) 984 WP_007579707.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55620.49 Da        Isoelectric Point: 4.8325

>NTDB_id=107920 AACK42_RS01730 WP_180721445.1 381500..383020(-) (rpoN/rpoN1) [Paraburkholderia sp. 22B1P]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVSMAIAQNPLLETEDDWIASPLRVAADGTVISQTSQNTSPEEMS
SAPPSSSSSSSSSESQEGGEPQGVDEYNGMGSDGNSDANQWNLDDYGRSGNASDDDDLPPLQIHESTTTLRDHLTAQLRV
TGASARDRALITFLIESLDDDGYLTATFDEILADLPEELEVDTDELNAALALLHSFDPAGVGARSASECLRLQLCRLEPS
PTRTLALDIVAHHLELLAARDFTRLRKYLKASDDDLREAHALIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELN
PEVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREI
ADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQG
FVVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=107920 AACK42_RS01730 WP_180721445.1 381500..383020(-) (rpoN/rpoN1) [Paraburkholderia sp. 22B1P]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTTGCGCTGACACCACAACTGCAACAGTCCATCCGGCTGCTGCA
GCTGTCTACGCTTGAGCTGCAACAGGAAGTGTCGATGGCGATCGCCCAGAATCCGTTGCTGGAAACGGAGGACGACTGGA
TCGCGAGTCCGTTGCGCGTTGCCGCCGACGGCACGGTCATCTCGCAGACGTCGCAGAACACCTCGCCGGAAGAGATGTCT
AGCGCGCCGCCGTCTTCGTCGTCGTCGAGCTCTTCCAGCGAAAGCCAGGAAGGCGGCGAGCCTCAAGGTGTGGACGAATA
CAACGGCATGGGTTCTGATGGGAACTCGGATGCCAACCAGTGGAATCTCGACGATTACGGCCGTTCGGGCAACGCATCGG
ACGACGACGATCTGCCGCCGCTGCAAATCCACGAATCGACCACGACGCTGCGCGATCACCTGACTGCTCAACTTCGCGTC
ACGGGAGCAAGCGCACGCGACCGCGCGCTCATCACGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACGGCCAC
CTTCGACGAAATCCTCGCTGATCTGCCCGAAGAACTCGAAGTCGATACCGACGAACTCAACGCGGCCCTCGCCTTGCTGC
ACAGCTTCGACCCAGCGGGCGTCGGCGCGCGTTCGGCGTCCGAATGCCTGCGTTTGCAGCTGTGCCGGCTCGAGCCTTCG
CCCACGCGCACGCTCGCGCTCGACATCGTCGCGCATCATCTGGAACTGCTTGCCGCACGCGACTTCACGCGCCTGCGCAA
ATACCTGAAGGCAAGCGACGACGATCTGCGCGAAGCCCATGCGCTGATCCGTTCGCTGGAGCCGTTCCCGGGCGCGGCCT
ACGGCAAGGCCGAAGCGGACTACGTCGTGCCCGACATCATGGTGCGCAAAACCGCACAGGGATGGCAGGCGGAACTGAAT
CCGGAAGTCGTGCCGAAACTGCGTATCAACCATCTGTACGCGAATATCCTGCGCAACAATCGCGGCGATCCGGGCAGCGG
TTCACTGCGGCAGCAACTGCAGGAAGCACGTTGGCTGATCAAGAATATCCAGCAGCGCTTCGAGACGATCCTGCGTGTCG
CACAGGCAATTGTGGAGCGTCAAAAGAACTTTTTCGCCCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATA
GCTGATACGCTGGGTTTACACGAGTCGACGGTCTCGCGTGTGACAACCGGCAAGTACATGCTCACCCCATTCGGGACGCT
TGAATTTAAGTACTTCTTCGGATCGCACGTGTCCACCGACACGGGGGGCGCGGCCTCTTCAACGGCCATTCGCGCACTCA
TCAAGCAACTGATAGGAGCGGAAGACACCAAATCTCCTCTTTCAGACAGCCGCATTGCCGAACTGCTGGCGGAACAGGGT
TTCGTCGTCGCTCGCCGTACCGTTGCGAAATACCGCGAAGCACTGAAGATCCCGGCAGTCAATCTGCGCAAGTCTCTTTA
G

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(349-504)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(143-333)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.996

100

0.67

Multiple sequence alignment