Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACFLKC_RS16960 Genome accession   NZ_CP175916
Coordinates   3689258..3690616 (-) Length   452 a.a.
NCBI ID   WP_163213176.1    Uniprot ID   -
Organism   Clostridium sp. FAM 27665     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3684258..3695616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFLKC_RS16940 (ACFLKC_16940) ispD 3685546..3686235 (-) 690 WP_053467242.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACFLKC_RS16945 (ACFLKC_16945) - 3686237..3687340 (-) 1104 WP_053467241.1 PIN/TRAM domain-containing protein -
  ACFLKC_RS16950 (ACFLKC_16950) - 3687748..3688146 (+) 399 WP_409069047.1 DUF1573 domain-containing protein -
  ACFLKC_RS16955 (ACFLKC_16955) disA 3688178..3689239 (-) 1062 WP_053469078.1 DNA integrity scanning diadenylate cyclase DisA -
  ACFLKC_RS16960 (ACFLKC_16960) radA 3689258..3690616 (-) 1359 WP_163213176.1 DNA repair protein RadA Machinery gene
  ACFLKC_RS16965 (ACFLKC_16965) - 3690736..3693171 (-) 2436 WP_409069048.1 ATP-dependent Clp protease ATP-binding subunit -
  ACFLKC_RS16970 (ACFLKC_16970) - 3693173..3694216 (-) 1044 WP_409069049.1 protein arginine kinase -
  ACFLKC_RS16975 (ACFLKC_16975) - 3694221..3694748 (-) 528 WP_409069050.1 UvrB/UvrC motif-containing protein -
  ACFLKC_RS16980 (ACFLKC_16980) - 3694771..3695235 (-) 465 WP_409069051.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49759.57 Da        Isoelectric Point: 7.2056

>NTDB_id=1079138 ACFLKC_RS16960 WP_163213176.1 3689258..3690616(-) (radA) [Clostridium sp. FAM 27665]
MAKNRIVYICQQCGYESIKWVGKCPGCNTWNSMVEEEKQSTDKNIKNLNIKSEPKIISNIKSSEYERLDTGINELNRVLG
GGIVKGSLTLISGSPGIGKSTILLQAANNIANKYGKVLYVSGEESEEQIKMRADRLKVISKDIYILSETNIDAIKEHITT
IDPKFVIIDSIQTLFKSELSSAPGTVSQVRQCSNDIMHISKNNNIPFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTVKNRFGTTSEIGVFEMAGEGLLEISNPSAVFLEETEFQREGSVIIGIMEGTRPILIEIQALVSETKAYMPRR
TAVGVDTARLNLILAVLEKKLNVPFYNCDVYVNVVGGLDLTGTFADLGLALALLSSAKSKDIKLPKVLVVGEIGLTGEVR
PVSFCDRIVNEGIKMGFTNIIIPDRNKDKISVKDNVNLIGISSLKEGISKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=1079138 ACFLKC_RS16960 WP_163213176.1 3689258..3690616(-) (radA) [Clostridium sp. FAM 27665]
ATGGCTAAAAATAGAATTGTTTATATATGTCAGCAATGTGGTTACGAATCTATAAAATGGGTGGGAAAATGTCCTGGATG
CAATACTTGGAATAGTATGGTAGAAGAGGAGAAACAATCAACTGATAAGAACATAAAAAACTTAAATATTAAATCGGAAC
CTAAAATAATATCCAACATAAAATCCAGTGAATATGAAAGATTAGATACTGGAATAAATGAACTTAATAGAGTCTTAGGT
GGCGGTATAGTTAAAGGTTCTTTAACTTTAATATCTGGGTCTCCCGGAATAGGGAAATCTACAATACTATTACAAGCTGC
TAATAATATTGCTAATAAGTATGGTAAGGTTTTATATGTATCAGGAGAAGAATCAGAAGAACAAATAAAAATGAGGGCAG
ATAGGCTTAAAGTAATATCAAAGGATATATACATACTTTCAGAAACAAATATAGATGCAATCAAAGAGCATATTACGACA
ATTGATCCTAAATTTGTTATAATAGATTCTATACAGACTCTTTTCAAGAGTGAACTATCTTCAGCTCCAGGGACTGTCTC
TCAAGTTAGGCAATGTTCTAACGATATTATGCATATAAGTAAAAATAATAATATACCGTTTTTTATAGTAGCACATGTTA
CAAAGCAAGGGGAACTAGCAGGACCTAGAGTTTTAGAACATATGGTAGATACAGTTTTATCTTTCGAAGGGGAAAGAACA
CAAGAATTTAGGATTTTAAGAACGGTAAAAAATCGTTTTGGAACTACAAGTGAAATAGGAGTCTTTGAAATGGCTGGAGA
AGGATTATTAGAAATAAGCAACCCTTCTGCTGTGTTTTTAGAAGAAACTGAATTTCAAAGGGAAGGTTCTGTAATAATAG
GTATAATGGAAGGAACTAGACCAATACTTATAGAAATACAGGCATTGGTAAGTGAAACAAAAGCTTATATGCCAAGGAGA
ACGGCAGTAGGAGTAGATACAGCTAGACTAAATTTAATATTAGCTGTGTTAGAAAAAAAATTAAATGTACCTTTCTATAA
TTGTGATGTATATGTTAATGTAGTAGGAGGATTAGATTTAACAGGAACTTTTGCTGATTTAGGATTAGCATTAGCCTTAT
TATCTAGCGCTAAATCAAAAGATATAAAATTACCTAAAGTTTTAGTAGTTGGAGAAATAGGACTCACAGGAGAGGTTCGT
CCAGTAAGTTTTTGTGATAGAATAGTAAATGAAGGAATAAAAATGGGGTTCACTAATATAATTATACCCGATAGAAATAA
AGATAAAATTTCTGTTAAAGATAATGTTAATTTAATTGGTATATCTTCTTTAAAAGAAGGTATTAGTAAAGTATTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

AAA domain (AAA_25)

(59-217)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.669

100

0.498
  radA Streptococcus pneumoniae Rx1

48.678

100

0.489
  radA Streptococcus pneumoniae D39

48.678

100

0.489
  radA Streptococcus pneumoniae R6

48.678

100

0.489
  radA Streptococcus pneumoniae TIGR4

48.678

100

0.489
  radA Streptococcus mitis NCTC 12261

48.678

100

0.489
  radA Streptococcus mitis SK321

48.678

100

0.489

Multiple sequence alignment