Detailed information NTG0107844

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ECOLIN_RS22655
• Genome accession: NZ_CP007799
• Coordinates: 4547445..4549643 (-)
• Length: 732 a.a.
• NCBI ID: WP_001305041.1
• Uniprot ID: A0A0H3ERG8
• Organism: Escherichia coli Nissle 1917
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4542445..4554643

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECOLIN_RS22635 (ECOLIN_21890)	hslU	4543249..4544580 (-)	1332	WP_001293344.1	HslU--HslV peptidase ATPase subunit	-
ECOLIN_RS22640 (ECOLIN_21895)	hslV	4544590..4545120 (-)	531	WP_000208242.1	ATP-dependent protease subunit HslV	-
ECOLIN_RS22645 (ECOLIN_21900)	ftsN	4545213..4546172 (-)	960	WP_000068846.1	cell division protein FtsN	-
ECOLIN_RS22650 (ECOLIN_21905)	cytR	4546264..4547289 (-)	1026	WP_000644904.1	DNA-binding transcriptional regulator CytR	Regulator
ECOLIN_RS22655 (ECOLIN_21910)	priA	4547445..4549643 (-)	2199	WP_001305041.1	primosomal protein N'	Machinery gene
ECOLIN_RS22660 (ECOLIN_21915)	rpmE	4549846..4550058 (+)	213	WP_000710769.1	50S ribosomal protein L31	-
ECOLIN_RS22665 (ECOLIN_21920)	yiiX	4550119..4550727 (-)	609	WP_000702296.1	YiiX family permuted papain-like enzyme	-
ECOLIN_RS22670 (ECOLIN_21925)	metJ	4550787..4551104 (-)	318	WP_000852812.1	met regulon transcriptional regulator MetJ	-
ECOLIN_RS31740 (ECOLIN_21930)	-	4551381..4551656 (+)	276	Protein_4357	PLP-dependent transferase	-
ECOLIN_RS22680 (ECOLIN_21935)	-	4551659..4551946 (-)	288	Protein_4358	primosomal protein N'	-
ECOLIN_RS22685 (ECOLIN_21940)	rpmE	4552149..4552361 (+)	213	WP_000710769.1	50S ribosomal protein L31	-
ECOLIN_RS22690 (ECOLIN_21945)	-	4552422..4553030 (-)	609	WP_000702296.1	YiiX family permuted papain-like enzyme	-
ECOLIN_RS22695 (ECOLIN_21950)	metJ	4553090..4553407 (-)	318	WP_000852812.1	met regulon transcriptional regulator MetJ	-
ECOLIN_RS31745 (ECOLIN_21955)	-	4553684..4553959 (+)	276	Protein_4362	PLP-dependent transferase	-
ECOLIN_RS22705 (ECOLIN_21960)	-	4553962..4554249 (-)	288	Protein_4363	primosomal protein N'	-

Sequence

Protein

• Length: 732 a.a.
• Molecular weight: 81666.95 Da
• Isoelectric Point: 9.1267

>NTDB_id=107844 ECOLIN_RS22655 WP_001305041.1 4547445..4549643(-) (priA) [Escherichia coli Nissle 1917]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILASPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide

• Length: 2199 bp

>NTDB_id=107844 ECOLIN_RS22655 WP_001305041.1 4547445..4549643(-) (priA) [Escherichia coli Nissle 1917]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGAGTCGCGTGCGCGTGCCGTTTGGCAAACAACAGGAGCGTATCGGTGTTGTGGTGTCAGTGAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCTGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GTTCGCCAAAGCAACAACAGGCGCTGGCAGCGTTACGGCAAGGCAAAATCTGGCGTGATCAGGTCGCGGAACTTGAATTT
AATGATGCCGCGTTGCAGGCACTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGGTTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCGGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTCATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTTATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGCGCGCATAG
CGAGCAAATCCCAATTATTCTTGGCTCCGCAACGCCCGCACTGGAAACGTTATGCAACGTGCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCGGGCAATGCGCGCCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAATCG
TCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGCCACCATTGCGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCTTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTGTTCCCTGGTGTCCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTGCATCGCGGCGGCGCGA
GGATCTTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAACGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACATTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCACTGGCTGAACGACGCATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCATTGTTCCTGCAACAACTGCGTAATCTCATTCTCGCCAGCCCACTGGCAGA
CGAGAAACTGTGGGTTCTCGGTCCGGTTCCGGCGCTGGCCCCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTACAGCACATCATCAATGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTGCTGGATGTCGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (632-729)

• DEAD/DEAH box helicase (DEAD) (203-362)

• Helicase conserved C-terminal domain (Helicase_C) (522-589)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-101)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (445-471)

3D structure

• AlphaFold DB: A0A0H3ERG8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	45.183	100	0.455