Detailed information NTG0107828

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: ECOLIN_RS20260
• Genome accession: NZ_CP007799
• Coordinates: 4075403..4076386 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196477.1
• Uniprot ID: A0A0H2VBW7
• Organism: Escherichia coli Nissle 1917
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 4070403..4081386

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECOLIN_RS29335	-	4072035..4072142 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
ECOLIN_RS31690	-	4072518..4072625 (-)	108	WP_000170738.1	type I toxin-antitoxin system toxin Ldr family protein	-
ECOLIN_RS20250 (ECOLIN_19570)	yhjV	4073101..4074372 (+)	1272	WP_001298005.1	aromatic amino acid transport family protein	-
ECOLIN_RS20255 (ECOLIN_19575)	dppF	4074402..4075406 (-)	1005	WP_000103580.1	dipeptide ABC transporter ATP-binding subunit DppF	-
ECOLIN_RS20260 (ECOLIN_19580)	amiE	4075403..4076386 (-)	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	Regulator
ECOLIN_RS20265 (ECOLIN_19585)	dppC	4076397..4077299 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
ECOLIN_RS20270 (ECOLIN_19590)	dppB	4077309..4078328 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
ECOLIN_RS32110	-	4078346..4078573 (+)	228	WP_000198577.1	hypothetical protein	-
ECOLIN_RS20280 (ECOLIN_19595)	dppA	4078635..4080242 (-)	1608	WP_001222871.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35801.38 Da
• Isoelectric Point: 6.5813

>NTDB_id=107828 ECOLIN_RS20260 WP_001196477.1 4075403..4076386(-) (amiE) [Escherichia coli Nissle 1917]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=107828 ECOLIN_RS20260 WP_001196477.1 4075403..4076386(-) (amiE) [Escherichia coli Nissle 1917]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGTGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAATCGGTCAGTTCGCTGGCAATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCAATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAAGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCTGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGACCAAAACTGCTGATTGCCGATGAACCGACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATTATCGTGATGTATGCAGGCCAGGTAGTAGAAACGGGCGATGCGCACGCT
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTACGCCA
CTGACAAATGCCGTGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (24-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

3D structure

• AlphaFold DB: A0A0H2VBW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404