Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACJU26_RS13355 Genome accession   NZ_CP175559
Coordinates   2571727..2572923 (-) Length   398 a.a.
NCBI ID   WP_414040117.1    Uniprot ID   -
Organism   Acidithiobacillus sp. M4-SHS-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2566727..2577923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJU26_RS13325 (ACJU26_13325) hslV 2566928..2567467 (+) 540 WP_414040111.1 ATP-dependent protease subunit HslV -
  ACJU26_RS13330 (ACJU26_13330) hslU 2567470..2568792 (+) 1323 WP_414040112.1 ATP-dependent protease ATPase subunit HslU -
  ACJU26_RS13335 (ACJU26_13335) - 2568829..2569149 (-) 321 WP_414040113.1 hypothetical protein -
  ACJU26_RS13340 (ACJU26_13340) rpoH 2569157..2570017 (-) 861 WP_414040114.1 RNA polymerase sigma factor RpoH -
  ACJU26_RS13345 (ACJU26_13345) ftsX 2570154..2571062 (-) 909 WP_414040115.1 permease-like cell division protein FtsX -
  ACJU26_RS13350 (ACJU26_13350) ftsE 2571059..2571730 (-) 672 WP_414040116.1 cell division ATP-binding protein FtsE -
  ACJU26_RS13355 (ACJU26_13355) pilA 2571727..2572923 (-) 1197 WP_414040117.1 signal recognition particle-docking protein FtsY Machinery gene
  ACJU26_RS13360 (ACJU26_13360) rsmD 2573040..2573612 (+) 573 WP_414040118.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACJU26_RS13365 (ACJU26_13365) coaD 2573609..2574130 (+) 522 WP_414040119.1 pantetheine-phosphate adenylyltransferase -
  ACJU26_RS13370 (ACJU26_13370) - 2574159..2574410 (+) 252 WP_215842621.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ACJU26_RS13375 (ACJU26_13375) mutM 2574447..2575259 (-) 813 WP_414040120.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  ACJU26_RS13380 (ACJU26_13380) prmC 2575252..2576094 (-) 843 WP_414041986.1 peptide chain release factor N(5)-glutamine methyltransferase -
  ACJU26_RS13385 (ACJU26_13385) prfA 2576087..2577163 (-) 1077 WP_414041987.1 peptide chain release factor 1 -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 42466.35 Da        Isoelectric Point: 5.0965

>NTDB_id=1078201 ACJU26_RS13355 WP_414040117.1 2571727..2572923(-) (pilA) [Acidithiobacillus sp. M4-SHS-6]
MVFNWFKRNKSPAPEERGEASETAPVPQELEALSRNSIPEAVEPTEKSATAEDVAASPSGLLPEPAAEAIPEAAAEDALS
QPQPAAASAPTKSMFARLREGLSRSREQFTEGVSRLVLGKKVIDEDLLEDLEALLLQADLGSAATQEIMSAVTESVRRKE
LTDPAALKKALRNALLDILRPRAETWVPEKGRTQVLMMVGINGAGKTTTTGKLAARWKAEGFSLMLAAGDTFRAAAVEQL
QGWGQRVQVPVVAQGTGADSASVIFDAFTAARARGADLLIADTAGRLHTQNHLMEELKKVKRVLGKQDPDAPHQVWLVLD
AGTGQNALNQAQQFHQAIGLTGICITKLDGTAKGGVVAAIAKTLPIPIRYIGMGEQVADLRPFDPESFVDALFAETKS

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=1078201 ACJU26_RS13355 WP_414040117.1 2571727..2572923(-) (pilA) [Acidithiobacillus sp. M4-SHS-6]
ATGGTTTTTAACTGGTTCAAGCGTAACAAAAGCCCCGCTCCGGAGGAACGGGGTGAGGCATCCGAAACCGCTCCTGTCCC
TCAGGAGCTTGAGGCGCTTTCCAGGAACAGTATCCCCGAAGCCGTGGAACCAACTGAAAAATCGGCAACAGCTGAGGACG
TTGCTGCTTCTCCATCGGGTCTGCTGCCGGAACCGGCAGCCGAAGCAATACCGGAGGCAGCTGCAGAGGATGCGCTTTCC
CAGCCCCAGCCCGCGGCGGCCAGTGCTCCCACAAAAAGCATGTTCGCGCGTCTGCGCGAGGGTCTGAGTCGCAGTCGGGA
ACAGTTCACCGAAGGCGTCAGCCGGCTGGTTCTGGGCAAGAAAGTCATCGACGAAGACTTGCTGGAAGATCTGGAAGCCC
TGCTTTTGCAAGCAGATCTGGGTTCGGCGGCGACCCAGGAAATCATGTCTGCCGTGACCGAAAGCGTCCGCCGCAAGGAA
TTGACGGATCCCGCCGCACTCAAAAAAGCCCTGCGCAACGCCCTGCTGGACATCCTGCGACCCCGGGCCGAAACCTGGGT
GCCGGAAAAAGGGCGGACCCAGGTGCTGATGATGGTCGGTATCAATGGCGCGGGAAAAACCACGACCACCGGTAAACTCG
CGGCACGTTGGAAAGCCGAGGGCTTTTCCCTGATGCTCGCCGCTGGTGATACCTTCCGGGCGGCGGCGGTGGAACAATTA
CAAGGTTGGGGTCAGCGTGTTCAGGTTCCAGTGGTCGCCCAGGGAACCGGGGCAGACAGTGCCTCGGTCATTTTTGATGC
CTTCACTGCCGCCCGTGCCCGGGGCGCCGACCTGCTCATTGCCGATACGGCTGGTCGCTTGCACACCCAGAACCACTTGA
TGGAAGAGCTCAAAAAGGTCAAGCGGGTACTGGGCAAGCAGGACCCGGATGCCCCCCATCAGGTCTGGCTGGTGCTGGAT
GCGGGCACCGGACAGAATGCCCTGAATCAGGCCCAGCAGTTTCACCAAGCTATCGGCCTGACCGGTATTTGTATTACCAA
GCTGGATGGTACCGCCAAGGGCGGGGTGGTGGCGGCCATTGCCAAGACGCTGCCTATCCCGATCCGCTATATTGGTATGG
GTGAGCAGGTTGCCGACCTGCGCCCCTTTGATCCCGAGTCTTTTGTGGATGCTCTTTTCGCGGAAACGAAATCATGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(194-393)

SRP54-type protein, helical bundle domain (SRP54_N)

(112-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.255

100

0.497

Multiple sequence alignment