Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACKFA7_RS08395 Genome accession   NZ_CP175553
Coordinates   1782282..1782716 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus amyloliquefaciens strain 4-9-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1777282..1787716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKFA7_RS08390 (ACKFA7_08390) - 1780782..1781993 (-) 1212 WP_038463906.1 cytochrome P450 -
  ACKFA7_RS08395 (ACKFA7_08395) nucA/comI 1782282..1782716 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACKFA7_RS08400 (ACKFA7_08400) - 1782776..1783531 (+) 756 WP_003154084.1 YoaK family protein -
  ACKFA7_RS08405 (ACKFA7_08405) - 1783565..1783927 (-) 363 WP_007410383.1 hypothetical protein -
  ACKFA7_RS08410 (ACKFA7_08410) - 1784120..1785448 (-) 1329 WP_038463909.1 S8 family peptidase -
  ACKFA7_RS08415 (ACKFA7_08415) - 1785627..1785858 (+) 232 Protein_1610 hypothetical protein -
  ACKFA7_RS08420 (ACKFA7_08420) - 1786114..1786821 (+) 708 WP_038463915.1 poly-gamma-glutamate hydrolase family protein -
  ACKFA7_RS08425 (ACKFA7_08425) - 1786881..1787333 (+) 453 WP_038463918.1 OsmC family protein -
  ACKFA7_RS08430 (ACKFA7_08430) - 1787347..1787700 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=1078141 ACKFA7_RS08395 WP_007611576.1 1782282..1782716(+) (nucA/comI) [Bacillus amyloliquefaciens strain 4-9-2]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1078141 ACKFA7_RS08395 WP_007611576.1 1782282..1782716(+) (nucA/comI) [Bacillus amyloliquefaciens strain 4-9-2]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATAGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment