Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACKFA7_RS01645 Genome accession   NZ_CP175553
Coordinates   301576..302013 (-) Length   145 a.a.
NCBI ID   WP_038456921.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain 4-9-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 296576..307013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKFA7_RS01620 (ACKFA7_01620) - 297755..298957 (+) 1203 WP_038456918.1 GTP-binding protein -
  ACKFA7_RS01625 (ACKFA7_01625) - 299022..300158 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  ACKFA7_RS01630 (ACKFA7_01630) - 300173..300607 (+) 435 WP_021494203.1 RDD family protein -
  ACKFA7_RS01635 (ACKFA7_01635) - 300724..301002 (+) 279 WP_263612330.1 YckD family protein -
  ACKFA7_RS01640 (ACKFA7_01640) nin/comJ 301157..301555 (-) 399 WP_038456919.1 competence protein ComJ Regulator
  ACKFA7_RS01645 (ACKFA7_01645) nucA/comI 301576..302013 (-) 438 WP_038456921.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACKFA7_RS01650 (ACKFA7_01650) hxlB 302370..302927 (-) 558 WP_038456922.1 6-phospho-3-hexuloisomerase -
  ACKFA7_RS01655 (ACKFA7_01655) hxlA 302924..303559 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  ACKFA7_RS01660 (ACKFA7_01660) - 303791..304153 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16099.38 Da        Isoelectric Point: 8.4850

>NTDB_id=1078113 ACKFA7_RS01645 WP_038456921.1 301576..302013(-) (nucA/comI) [Bacillus amyloliquefaciens strain 4-9-2]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKNDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1078113 ACKFA7_RS01645 WP_038456921.1 301576..302013(-) (nucA/comI) [Bacillus amyloliquefaciens strain 4-9-2]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAATGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment