Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACJ51O_RS15350 Genome accession   NZ_CP174521
Coordinates   3271920..3273425 (+) Length   501 a.a.
NCBI ID   WP_034179060.1    Uniprot ID   A0A2S8I6S2
Organism   Burkholderia pyrrocinia strain DLL-114     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3266920..3278425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ51O_RS15325 (ACJ51O_15325) kdsD 3268163..3269146 (+) 984 WP_105393307.1 arabinose 5-phosphate isomerase KdsD -
  ACJ51O_RS15330 (ACJ51O_15330) - 3269146..3269682 (+) 537 WP_407970887.1 KdsC family phosphatase -
  ACJ51O_RS15335 (ACJ51O_15335) lptC 3269686..3270300 (+) 615 WP_034179057.1 LPS export ABC transporter periplasmic protein LptC -
  ACJ51O_RS15340 (ACJ51O_15340) lptA 3270321..3270986 (+) 666 WP_407970888.1 lipopolysaccharide transport periplasmic protein LptA -
  ACJ51O_RS15345 (ACJ51O_15345) lptB 3270983..3271759 (+) 777 WP_034179059.1 LPS export ABC transporter ATP-binding protein -
  ACJ51O_RS15350 (ACJ51O_15350) rpoN/rpoN1 3271920..3273425 (+) 1506 WP_034179060.1 RNA polymerase factor sigma-54 Machinery gene
  ACJ51O_RS15355 (ACJ51O_15355) hpf 3273585..3273944 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACJ51O_RS15360 (ACJ51O_15360) - 3274141..3274653 (+) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  ACJ51O_RS15365 (ACJ51O_15365) hprK 3274783..3275751 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  ACJ51O_RS15370 (ACJ51O_15370) rapZ 3275827..3276735 (+) 909 WP_034179062.1 RNase adapter RapZ -
  ACJ51O_RS15375 (ACJ51O_15375) - 3276763..3277398 (+) 636 WP_034179063.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54843.96 Da        Isoelectric Point: 4.8740

>NTDB_id=1077772 ACJ51O_RS15350 WP_034179060.1 3271920..3273425(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain DLL-114]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIVSPLRVAADGSLIAQTPSSQNTEPAP
ANSSSTSGDRAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1077772 ACJ51O_RS15350 WP_034179060.1 3271920..3273425(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain DLL-114]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGTGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACACCTTCCAGCCAGAATACCGAGCCGGCCCCG
GCAAACAGCAGCAGCACGTCGGGCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGTGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTGGAGTTGCTCGCCGCACGCGACTTCACGCGATTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGACCGGTTCGCTGAAACAGCA
GCTTCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAAAAGAATTTCTTTGCACATGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S8I6S2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.713

100

0.693

Multiple sequence alignment