Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   ACJGE4_RS11965 Genome accession   NZ_CP174519
Coordinates   2482738..2483538 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain JD-3     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2477738..2488538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJGE4_RS11935 (ACJGE4_11935) - 2478275..2478907 (+) 633 WP_007408365.1 helix-turn-helix domain-containing protein -
  ACJGE4_RS11940 (ACJGE4_11940) - 2479204..2479341 (+) 138 WP_162839613.1 hypothetical protein -
  ACJGE4_RS11945 (ACJGE4_11945) - 2479435..2480553 (+) 1119 WP_032863662.1 NADH-dependent flavin oxidoreductase -
  ACJGE4_RS11950 (ACJGE4_11950) - 2480681..2480893 (+) 213 WP_007408362.1 spore germination protein -
  ACJGE4_RS11955 (ACJGE4_11955) - 2481019..2481231 (+) 213 WP_003153181.1 spore germination protein -
  ACJGE4_RS11960 (ACJGE4_11960) alr 2481408..2482589 (+) 1182 WP_094031829.1 alanine racemase -
  ACJGE4_RS11965 (ACJGE4_11965) spo0A 2482738..2483538 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  ACJGE4_RS11970 (ACJGE4_11970) spoIVB 2483819..2485099 (-) 1281 WP_043020798.1 SpoIVB peptidase -
  ACJGE4_RS11975 (ACJGE4_11975) recN 2485263..2486993 (-) 1731 WP_007408359.1 DNA repair protein RecN Machinery gene
  ACJGE4_RS11980 (ACJGE4_11980) ahrC 2487025..2487474 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  ACJGE4_RS11985 (ACJGE4_11985) - 2487571..2488416 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=1077722 ACJGE4_RS11965 WP_003153177.1 2482738..2483538(-) (spo0A) [Bacillus velezensis strain JD-3]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1077722 ACJGE4_RS11965 WP_003153177.1 2482738..2483538(-) (spo0A) [Bacillus velezensis strain JD-3]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCTGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACTGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTCAGC
ATGACAAAAGCAAAACCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment