Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACJJWD_RS24065 Genome accession   NZ_CP174505
Coordinates   5283073..5284674 (-) Length   533 a.a.
NCBI ID   WP_407714533.1    Uniprot ID   -
Organism   Comamonas testosteroni strain VSPL.dLG     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5278073..5289674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJWD_RS24045 (ACJJWD_24065) - 5278321..5279553 (+) 1233 WP_407714530.1 DUF711 family protein -
  ACJJWD_RS24050 (ACJJWD_24070) - 5279688..5280614 (+) 927 WP_080731616.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  ACJJWD_RS24055 (ACJJWD_24075) - 5281108..5281674 (+) 567 WP_407714531.1 hypothetical protein -
  ACJJWD_RS24060 (ACJJWD_24080) - 5281718..5282992 (+) 1275 WP_407714532.1 YfaP family protein -
  ACJJWD_RS24065 (ACJJWD_24085) rpoN/rpoN1 5283073..5284674 (-) 1602 WP_407714533.1 RNA polymerase factor sigma-54 Machinery gene
  ACJJWD_RS24070 (ACJJWD_24090) lptB 5284820..5285602 (-) 783 WP_407714534.1 LPS export ABC transporter ATP-binding protein -
  ACJJWD_RS24075 (ACJJWD_24095) lptA 5285599..5286255 (-) 657 WP_211457385.1 lipopolysaccharide transport periplasmic protein LptA -
  ACJJWD_RS24080 (ACJJWD_24100) - 5286387..5287037 (-) 651 WP_043003858.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACJJWD_RS24085 (ACJJWD_24105) - 5287193..5287894 (-) 702 WP_043003857.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 58983.29 Da        Isoelectric Point: 4.6632

>NTDB_id=1077458 ACJJWD_RS24065 WP_407714533.1 5283073..5284674(-) (rpoN/rpoN1) [Comamonas testosteroni strain VSPL.dLG]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAEAAGDAPVDVAASASEYSVSDSIHAE
KEASQAQLATETVAEAPNPETPETLNWEADGMDGQDGEWGGESSSEWDTPSASSSTRNRDGDDETDAIDLAREHQSLTDF
LHRQALSLHLGAADTAALRFLIESLSDDGYLEDSLQELAQSLAGDDLEEREELEQHFAIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRTAEDEGTTEAHMSRLLLAQAICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVVPDVIVRPVRSGSGRADFSVQLNPEVMPRLRVHDIYAGVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDARKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=1077458 ACJJWD_RS24065 WP_407714533.1 5283073..5284674(-) (rpoN/rpoN1) [Comamonas testosteroni strain VSPL.dLG]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTATCGCTGACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTCTCCACTCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAACGTGCGGGCGATGAGG
CCGAAGCTGCGGGAGATGCTCCTGTAGATGTAGCTGCTAGCGCCAGTGAATACAGTGTTTCAGATAGTATTCATGCTGAA
AAAGAAGCATCGCAAGCGCAATTAGCTACCGAAACCGTAGCAGAAGCGCCGAATCCCGAAACCCCGGAAACGCTGAACTG
GGAGGCCGACGGCATGGACGGCCAGGACGGGGAATGGGGCGGCGAATCCAGCAGCGAGTGGGATACGCCCTCGGCCAGCA
GCTCCACGCGCAACCGCGATGGCGATGACGAAACCGATGCCATCGATCTGGCGCGCGAGCACCAGAGCCTGACCGACTTT
CTCCATCGTCAGGCGCTGAGCCTGCATCTGGGCGCGGCAGACACGGCGGCGCTGCGCTTTCTCATCGAGTCGCTCAGCGA
CGACGGCTATCTCGAGGATTCCCTGCAGGAGCTGGCGCAGAGCCTGGCCGGCGACGATCTGGAAGAGCGCGAGGAGCTGG
AGCAGCACTTTGCCATTGCGCTGAAGCTGCTGCAAAGCCTGGAGCCGGTGGGCGTGGGCGCGCGCGATCTGGCCGAGTGC
CTGCAACTGCAGCTGCGCGATCTGATCCGCACGGCCGAGGACGAGGGTACGACCGAGGCGCATATGAGCCGGCTGCTGCT
GGCGCAGGCCATCTGTGCCCAGCCCATGGACCTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGCGGCGCGC
GCGAGGAACAGCTGCGCGAGGCCATGGCCCTGATCGCCAGACTGGAGCCGCGTCCGGGTCGGCGCTTTGTGGACGTGGAG
CGCCATATCGTGGTGCCCGATGTCATCGTGCGCCCGGTGCGCTCCGGCTCGGGCCGGGCGGACTTTTCCGTGCAGCTCAA
TCCCGAGGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCGGAGTGCTGCGTCGCCACAAGAACAGTGACGGTCATG
CGGCACTGCAGCAGCAGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCTTGCGTGTT
TCCGAAGCCATCGTGGAACGGCAGAAGAACTTCTTTGTGCATGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGATAT
CGCCGACACGCTTGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCCAAGGTACTT
TCGAGCTCAAGTATTTCTTCGGTTCGGGCCTGGGCACGGAAACCGGCGGCAGTGCCTCCAGCACGGCCGTGCGTGCGCTG
ATCAAACAGTTTGTCGATGCCGAAGATGCCAGAAAGCCGCTGTCGGACGCCAAGCTGGCCGACATGCTCAAGGAGCAGGG
CATTGAGTGTGCGCGCCGCACCGTCGCCAAATACCGCGAGCAGCTCAAGATACCCACGACCACGCTGCGCAAGGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.28

100

0.505

Multiple sequence alignment