Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACJJWD_RS00585 Genome accession   NZ_CP174505
Coordinates   134348..135733 (-) Length   461 a.a.
NCBI ID   WP_003059793.1    Uniprot ID   -
Organism   Comamonas testosteroni strain VSPL.dLG     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 129348..140733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJWD_RS00560 (ACJJWD_00560) - 130422..131186 (+) 765 WP_407714761.1 glycosyltransferase family 25 protein -
  ACJJWD_RS00565 (ACJJWD_00565) - 131176..132261 (-) 1086 WP_407714763.1 glycosyltransferase -
  ACJJWD_RS00570 (ACJJWD_00570) - 132342..132551 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  ACJJWD_RS00575 (ACJJWD_00575) - 132692..133630 (-) 939 WP_003059799.1 branched-chain amino acid transaminase -
  ACJJWD_RS00580 (ACJJWD_00580) - 133712..134167 (-) 456 WP_211458096.1 glycerate kinase -
  ACJJWD_RS00585 (ACJJWD_00585) radA 134348..135733 (-) 1386 WP_003059793.1 DNA repair protein RadA Machinery gene
  ACJJWD_RS00595 (ACJJWD_00595) - 136204..137511 (-) 1308 WP_043003323.1 Glu/Leu/Phe/Val dehydrogenase -
  ACJJWD_RS00600 (ACJJWD_00600) - 137721..138644 (+) 924 WP_407714764.1 fumarylacetoacetate hydrolase family protein -
  ACJJWD_RS00605 (ACJJWD_00605) - 138804..139667 (-) 864 WP_407714765.1 PPK2 family polyphosphate kinase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48059.37 Da        Isoelectric Point: 7.6670

>NTDB_id=1077445 ACJJWD_RS00585 WP_003059793.1 134348..135733(-) (radA) [Comamonas testosteroni strain VSPL.dLG]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNSLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTIAVVPKANAPKKPIAGLTIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1077445 ACJJWD_RS00585 WP_003059793.1 134348..135733(-) (radA) [Comamonas testosteroni strain VSPL.dLG]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCGCGCTGGCTGGGCAAATGCCCGGGCTG
CGGCGCCTGGAACAGCCTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAACCGCCTGAGCCAGCCCCAGGGCT
ATGCGGGTCTGGCCAATGCCCAGCCCGTCACCCCGCTGTCGGCCATCGAGGCCCAGGATGTGGCGCGCACCCCCAGCGGC
ATCGAGGAGCTGGACCGGGTGCTGGGCGGCGGCGTGGTCGAAGGCGGCGTGGTTCTGATCGGCGGCGACCCCGGCATAGG
TAAGTCCACACTTCTTTTGCAGGCCATGGATGCATTGCACCGTATCGGCCTGCCCACGCTCTATGTGACGGGCGAGGAAA
GCGGCGCCCAGGTGGCGCTGCGCTCGCGCCGGCTCGGGCTGGACAACAGCCAGGTCAATGTGCTGGCCGAGATCCAGCTC
GAAAAAATCCTGGCCACCGTGGAGGCCACCCAGCCCGCCGTGGTGGTCATCGACTCGATCCAGACCGTGTATTCGGACCA
GCTCTCCTCGGCTCCGGGCTCCGTGGCCCAGGTGCGCGAATGCGCGGCCCATCTCACGCGTGCGGCCAAGACCACGGGCA
TCACCATCATTCTGGTGGGCCATGTGACCAAGGACGGCGCGCTGGCCGGCCCGCGCGTGCTCGAGCACATGGTGGACACG
GTGCTCTATTTCGAAGGCGACACGCACAGCAACTTCCGCCTGGTGCGCGCCATCAAGAACCGCTTCGGTGCCGTCAACGA
GATCGGCGTCTTCGCGATGACGGAAAAAGGCCTCAAGGGCGTGACCAACCCCAGCGCCATCTTTCTGAGCCAGCACAGCG
AGCCCGTGCCCGGCAGCTGCGTGCTGGTGACGCTGGAGGGCACGCGCCCCATGCTGGTGGAAATCCAGGCCCTGGTGGAC
CAGGGCGGCCCGGCTGCGCGTCGCCTCTCGGTCGGCCTGGACCGCGACCGGCTGGCCATGCTGCTGGCCGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGACCAGGATGTCTTCGTCAATGCCGTGGGCGGCGTGCGCATCAACGAGCCGGCAGCCG
ACTTAGCGGTGATGTTGGCAATCACTTCCAGCCTGCGCGGCAAGTCGCTGCCCAAGGGCTTTATCGCGTTTGGCGAGGTC
GGTCTGGCCGGCGAGGTGCGCCCTGCACCGCGCGGTCAGGAGCGACTCAAGGAGGCGGCAAAGTTGGGATTCACTATCGC
CGTGGTTCCCAAGGCCAATGCCCCCAAGAAGCCCATCGCCGGCCTGACCATCCATGCCGTGGAGCGCGTGGATGAGGCCA
TCAACATCGTGCGCGGCATTGGCTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(364-455)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.802

99.566

0.486
  radA Streptococcus mitis NCTC 12261

47.817

99.349

0.475
  radA Streptococcus pneumoniae D39

48.661

97.18

0.473
  radA Streptococcus pneumoniae TIGR4

48.661

97.18

0.473
  radA Streptococcus pneumoniae R6

48.661

97.18

0.473
  radA Streptococcus pneumoniae Rx1

48.661

97.18

0.473
  radA Streptococcus mitis SK321

49.655

94.36

0.469

Multiple sequence alignment