Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACJMCD_RS19520 Genome accession   NZ_CP174470
Coordinates   3683863..3684237 (+) Length   124 a.a.
NCBI ID   WP_407728419.1    Uniprot ID   -
Organism   Priestia megaterium strain WSY10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3678863..3689237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJMCD_RS19500 (ACJMCD_19500) - 3679354..3680025 (-) 672 WP_013058558.1 response regulator transcription factor -
  ACJMCD_RS19505 (ACJMCD_19505) - 3680351..3681169 (+) 819 WP_013058559.1 squalene/phytoene synthase family protein -
  ACJMCD_RS19510 (ACJMCD_19510) - 3681205..3683154 (-) 1950 WP_053487648.1 FUSC family protein -
  ACJMCD_RS19515 (ACJMCD_19515) - 3683310..3683570 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  ACJMCD_RS19520 (ACJMCD_19520) nucA/comI 3683863..3684237 (+) 375 WP_407728419.1 sporulation protein Machinery gene
  ACJMCD_RS19525 (ACJMCD_19525) comJ 3684273..3684680 (+) 408 WP_064472036.1 competence protein ComJ -
  ACJMCD_RS19530 (ACJMCD_19530) helD 3684709..3687027 (-) 2319 WP_064472037.1 RNA polymerase recycling motor HelD -
  ACJMCD_RS19535 (ACJMCD_19535) - 3687272..3687397 (-) 126 WP_016765178.1 hypothetical protein -
  ACJMCD_RS19540 (ACJMCD_19540) - 3687537..3688055 (-) 519 WP_223268980.1 enoyl-CoA hydratase/isomerase family protein -
  ACJMCD_RS19545 (ACJMCD_19545) - 3688295..3688663 (+) 369 WP_029323759.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13683.96 Da        Isoelectric Point: 4.4177

>NTDB_id=1077223 ACJMCD_RS19520 WP_407728419.1 3683863..3684237(+) (nucA/comI) [Priestia megaterium strain WSY10]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPHGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=1077223 ACJMCD_RS19520 WP_407728419.1 3683863..3684237(+) (nucA/comI) [Priestia megaterium strain WSY10]
ATGGGACATGCGGCGTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAACTACGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGGGAAGAATCGCTGAAAGGATATCCGACAAAAACAGGCTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGCGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCACATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.387

95.968

0.637

Multiple sequence alignment