Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACH36K_RS03035 Genome accession   NZ_CP174370
Coordinates   610237..611604 (+) Length   455 a.a.
NCBI ID   WP_411169506.1    Uniprot ID   -
Organism   Clostridium sp. MB05     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 605237..616604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH36K_RS03005 (ACH36K_03005) - 606173..606400 (+) 228 WP_322412497.1 glutaredoxin family protein -
  ACH36K_RS03010 (ACH36K_03010) - 606409..607869 (+) 1461 WP_411170648.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  ACH36K_RS03015 (ACH36K_03015) - 607950..608177 (-) 228 WP_411169502.1 glutaredoxin family protein -
  ACH36K_RS03020 (ACH36K_03020) - 608328..608489 (-) 162 WP_411169503.1 hypothetical protein -
  ACH36K_RS03025 (ACH36K_03025) - 608622..609344 (+) 723 WP_411169504.1 GntR family transcriptional regulator -
  ACH36K_RS03030 (ACH36K_03030) nagB 609341..610066 (+) 726 WP_411169505.1 glucosamine-6-phosphate deaminase -
  ACH36K_RS03035 (ACH36K_03035) radA 610237..611604 (+) 1368 WP_411169506.1 DNA repair protein RadA Machinery gene
  ACH36K_RS03040 (ACH36K_03040) disA 611621..612685 (+) 1065 WP_411169507.1 DNA integrity scanning diadenylate cyclase DisA -
  ACH36K_RS03045 (ACH36K_03045) - 612756..613151 (-) 396 WP_411169508.1 DUF1573 domain-containing protein -
  ACH36K_RS03050 (ACH36K_03050) - 613409..614500 (+) 1092 WP_411169509.1 PIN/TRAM domain-containing protein -
  ACH36K_RS03055 (ACH36K_03055) ispD 614516..615184 (+) 669 WP_411169510.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50496.33 Da        Isoelectric Point: 7.5802

>NTDB_id=1076909 ACH36K_RS03035 WP_411169506.1 610237..611604(+) (radA) [Clostridium sp. MB05]
MAKIKSMFVCQECGYESPKWLGKCPDCNKWNTLVEEIKETQKEIKSKVNNIRVLDNMPKSIGQIKSGEKQRYNTGLKELN
RVLGGGLVRGSLTLISGDPGIGKSTLLLQTANSISRTYGKVLYVSGEESEEQIKIRGDRLGANAEQLYILSETNLDLIEA
YINELKPVFVIIDSIQTVYKESVTSAPGSVSQVKECSNAIMRIGKNQNIPLFIVAHVTKQGELAGPRVLEHMVDTVLYFE
GERTEEFRVLRTMKNRFGTTSEIGVFEMAEEGLKEIYDPSRIFLEDTNFNQEGSAVIGIMEGTRPILVEMQALVSETNMH
MASRTAVGIDNQRLRLILAVLEKKLRVPFYKYDVYVNVVGGLSLDGTTGDLGLALALLSSVKNTGFKLERMVIVGEVGLT
GEIRPVSSCDRLIKEAEKMGFKNAIIPERNKDKVNSNSINIIGVSNLREAISKIF

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1076909 ACH36K_RS03035 WP_411169506.1 610237..611604(+) (radA) [Clostridium sp. MB05]
ATGGCTAAGATTAAATCCATGTTTGTTTGTCAAGAATGCGGATATGAATCTCCTAAATGGTTAGGGAAATGTCCAGATTG
TAACAAATGGAATACCTTAGTAGAAGAAATAAAAGAAACTCAAAAAGAAATTAAAAGCAAAGTAAATAATATAAGAGTTT
TAGATAATATGCCAAAGAGTATAGGACAAATTAAATCTGGAGAAAAACAACGATATAATACTGGCTTAAAAGAGTTAAAT
AGGGTTTTGGGTGGAGGCTTAGTAAGAGGGTCATTAACATTAATATCAGGAGATCCTGGAATAGGTAAGTCAACATTGTT
ACTACAAACAGCAAATTCAATATCAAGGACATATGGAAAAGTATTATATGTTTCTGGTGAAGAATCTGAAGAACAAATTA
AAATAAGAGGAGATAGATTAGGAGCTAATGCAGAGCAACTATATATATTATCAGAAACTAATCTTGACTTAATAGAAGCA
TACATAAATGAGTTAAAGCCTGTTTTCGTAATAATAGATTCAATACAAACTGTTTATAAAGAGTCTGTTACATCAGCTCC
AGGAAGTGTGTCTCAGGTAAAGGAATGCTCTAATGCTATAATGAGAATAGGCAAAAACCAAAATATACCATTGTTTATAG
TTGCTCATGTAACTAAACAAGGAGAACTTGCTGGGCCAAGAGTACTAGAGCATATGGTAGATACGGTATTATATTTTGAA
GGAGAACGTACAGAAGAGTTTAGAGTTCTTAGAACTATGAAAAATCGTTTTGGAACAACTAGCGAAATAGGTGTATTTGA
AATGGCTGAAGAAGGATTAAAGGAAATATATGATCCTTCGAGAATATTCTTAGAAGATACTAACTTTAATCAAGAGGGAT
CAGCTGTTATAGGCATAATGGAAGGAACTAGACCAATTCTTGTTGAAATGCAAGCTCTAGTTAGTGAAACAAATATGCAT
ATGGCTAGTAGAACCGCTGTTGGAATAGATAATCAAAGGTTAAGACTTATATTAGCAGTTTTAGAGAAAAAACTAAGAGT
GCCATTTTATAAGTATGATGTATATGTAAATGTAGTTGGTGGTCTTAGTTTAGATGGAACAACAGGAGACTTAGGGCTTG
CGTTAGCACTTTTATCAAGTGTAAAAAATACTGGATTTAAATTAGAAAGAATGGTCATAGTTGGAGAGGTTGGTCTTACT
GGTGAGATAAGACCCGTTTCATCTTGCGATAGGCTAATTAAAGAAGCTGAGAAGATGGGCTTTAAGAATGCTATTATTCC
AGAAAGAAATAAGGATAAAGTTAATAGTAATTCTATTAATATAATAGGAGTAAGCAATTTAAGAGAGGCTATTAGTAAGA
TATTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Subunit ChlI of Mg-chelatase (ChlI)

(318-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

52.98

99.56

0.527
  radA Streptococcus pneumoniae Rx1

47.609

100

0.481
  radA Streptococcus pneumoniae D39

47.609

100

0.481
  radA Streptococcus pneumoniae R6

47.609

100

0.481
  radA Streptococcus pneumoniae TIGR4

47.609

100

0.481
  radA Streptococcus mitis SK321

47.609

100

0.481
  radA Streptococcus mitis NCTC 12261

47.391

100

0.479

Multiple sequence alignment