Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACJA3J_RS00585 Genome accession   NZ_CP174345
Coordinates   112176..113555 (+) Length   459 a.a.
NCBI ID   WP_089654457.1    Uniprot ID   A0A3E0J2F3
Organism   Halobacillus sp. SY10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 107176..118555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJA3J_RS00565 (ACJA3J_00565) - 107335..107799 (+) 465 WP_089654461.1 CtsR family transcriptional regulator -
  ACJA3J_RS00570 (ACJA3J_00570) - 107820..108368 (+) 549 WP_089654460.1 UvrB/UvrC motif-containing protein -
  ACJA3J_RS00575 (ACJA3J_00575) - 108368..109438 (+) 1071 WP_089654459.1 protein arginine kinase -
  ACJA3J_RS00580 (ACJA3J_00580) clpC 109457..111916 (+) 2460 WP_089654458.1 ATP-dependent protease ATP-binding subunit ClpC -
  ACJA3J_RS00585 (ACJA3J_00585) radA 112176..113555 (+) 1380 WP_089654457.1 DNA repair protein RadA Machinery gene
  ACJA3J_RS00590 (ACJA3J_00590) disA 113556..114629 (+) 1074 WP_089654456.1 DNA integrity scanning diadenylate cyclase DisA -
  ACJA3J_RS00595 (ACJA3J_00595) - 114784..115878 (+) 1095 WP_115895010.1 PIN/TRAM domain-containing protein -
  ACJA3J_RS00600 (ACJA3J_00600) - 115977..117167 (+) 1191 WP_406945261.1 bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50287.83 Da        Isoelectric Point: 7.0909

>NTDB_id=1076762 ACJA3J_RS00585 WP_089654457.1 112176..113555(+) (radA) [Halobacillus sp. SY10]
MAKRKTKFVCQECGYETPKWMGKCPGCNQWNTLVEEMTAAPANSRHVFQTSSTSATTKPEKITQIKSQKEPRMPTDMPEF
NRVLGGGIVAGSLVLIGGDPGIGKSTLLLQVSAQIAKKDFPVLYISGEESSRQTKLRAERLDITSDELYVLSETNLQDVI
NQIENIEPRFVVIDSIQTIFKEDVTSAPGSVSQVRECTSHLMRIAKSKGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRSVKNRFGSTHEMGIFEMKEKGLEEVLNPSEIFLEERSQGAAGSIVVASMEGTRPVLVEIQALISPTAY
GNPRRMATGLDNSRVPLLMAVLEKRAGLLLQNQDAYVKVAGGVKLDEPAIDLAVAISIASSFRDQSSNGDDVVVGEVGLT
GEIRRVARIEQRVQEAAKLGFKRVIIPKKNMDGWTPPSQIEVIGVSTVQEAMKVTLGDS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=1076762 ACJA3J_RS00585 WP_089654457.1 112176..113555(+) (radA) [Halobacillus sp. SY10]
TTGGCAAAAAGGAAAACGAAGTTTGTTTGCCAGGAATGTGGGTATGAGACACCTAAATGGATGGGAAAGTGTCCGGGGTG
TAATCAATGGAACACGCTGGTCGAAGAAATGACGGCTGCTCCGGCGAATTCCAGACATGTGTTTCAGACAAGCTCCACTT
CCGCAACAACGAAACCAGAAAAAATCACACAAATCAAATCACAAAAAGAACCGCGTATGCCGACGGATATGCCTGAATTC
AACCGGGTTTTAGGTGGAGGAATCGTGGCGGGGTCTTTAGTTTTGATCGGCGGAGATCCGGGAATCGGTAAATCCACTCT
TTTACTCCAAGTCTCTGCGCAGATCGCCAAAAAAGATTTTCCTGTCCTCTACATATCCGGAGAGGAGTCATCGAGACAAA
CGAAGCTTCGAGCCGAACGTTTAGATATTACATCCGATGAACTCTATGTTCTATCGGAAACGAACTTACAGGATGTCATC
AACCAAATTGAAAATATTGAACCAAGGTTTGTGGTCATAGACTCGATCCAGACGATTTTCAAAGAAGACGTCACCTCTGC
TCCAGGGAGTGTTTCTCAAGTTCGTGAGTGTACGAGTCATTTGATGAGAATTGCTAAAAGCAAAGGTATTCCTATTTTCA
TCGTTGGGCATGTTACTAAAGAAGGTTCGATTGCCGGCCCACGTCTGCTTGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGGGAGCGGCATCATACATTTAGAATTCTAAGAAGTGTGAAGAACCGTTTCGGAAGCACTCATGAAATGGGAATCTT
CGAAATGAAGGAGAAAGGTCTGGAAGAGGTTCTGAACCCTTCAGAGATTTTCCTCGAAGAACGCTCGCAAGGGGCTGCAG
GTTCGATCGTAGTGGCTTCCATGGAAGGGACAAGGCCGGTACTTGTAGAAATTCAAGCCCTGATTTCACCTACAGCTTAC
GGCAACCCGAGACGGATGGCTACAGGCCTTGATAACAGCCGGGTACCGCTTTTAATGGCGGTCTTGGAAAAGAGAGCAGG
GCTCCTGCTTCAGAACCAGGATGCTTACGTAAAAGTCGCTGGTGGAGTGAAATTGGATGAGCCGGCGATTGACTTAGCGG
TTGCGATCAGCATTGCCTCAAGTTTCAGGGATCAGTCTTCGAATGGAGATGATGTCGTCGTTGGGGAAGTTGGCTTAACG
GGTGAGATACGAAGAGTGGCCAGAATTGAGCAAAGAGTCCAGGAAGCGGCGAAGTTAGGCTTTAAAAGGGTCATCATTCC
GAAGAAGAACATGGATGGCTGGACGCCTCCTTCTCAAATCGAAGTGATCGGTGTCAGTACCGTTCAGGAGGCGATGAAGG
TTACACTGGGGGATTCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E0J2F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.362

99.782

0.732
  radA Streptococcus mitis NCTC 12261

62.914

98.693

0.621
  radA Streptococcus mitis SK321

62.914

98.693

0.621
  radA Streptococcus pneumoniae Rx1

62.693

98.693

0.619
  radA Streptococcus pneumoniae D39

62.693

98.693

0.619
  radA Streptococcus pneumoniae R6

62.693

98.693

0.619
  radA Streptococcus pneumoniae TIGR4

62.693

98.693

0.619

Multiple sequence alignment