Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACJA3T_RS14600 Genome accession   NZ_CP174276
Coordinates   3131871..3133376 (+) Length   501 a.a.
NCBI ID   WP_059642617.1    Uniprot ID   A0AAW3Q401
Organism   Burkholderia semiarida strain SYBS718     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3126871..3138376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJA3T_RS14575 (ACJA3T_14575) kdsD 3128102..3129085 (+) 984 WP_059575664.1 arabinose 5-phosphate isomerase KdsD -
  ACJA3T_RS14580 (ACJA3T_14580) - 3129085..3129621 (+) 537 WP_059575662.1 HAD family hydrolase -
  ACJA3T_RS14585 (ACJA3T_14585) lptC 3129625..3130239 (+) 615 WP_059506659.1 LPS export ABC transporter periplasmic protein LptC -
  ACJA3T_RS14590 (ACJA3T_14590) lptA 3130260..3130925 (+) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  ACJA3T_RS14595 (ACJA3T_14595) lptB 3130922..3131698 (+) 777 WP_059575659.1 LPS export ABC transporter ATP-binding protein -
  ACJA3T_RS14600 (ACJA3T_14600) rpoN/rpoN1 3131871..3133376 (+) 1506 WP_059642617.1 RNA polymerase factor sigma-54 Machinery gene
  ACJA3T_RS14605 (ACJA3T_14605) hpf 3133536..3133895 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACJA3T_RS14610 (ACJA3T_14610) - 3134090..3134602 (+) 513 WP_179231743.1 PTS sugar transporter subunit IIA -
  ACJA3T_RS14615 (ACJA3T_14615) hprK 3134732..3135700 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  ACJA3T_RS14620 (ACJA3T_14620) rapZ 3135775..3136683 (+) 909 WP_406805596.1 RNase adapter RapZ -
  ACJA3T_RS14625 (ACJA3T_14625) - 3136711..3137346 (+) 636 WP_011546241.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54699.83 Da        Isoelectric Point: 4.8740

>NTDB_id=1076739 ACJA3T_RS14600 WP_059642617.1 3131871..3133376(+) (rpoN/rpoN1) [Burkholderia semiarida strain SYBS718]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPASNTEPAA
ANGTSTSSDRAERDEPAGADEYDGYASGDGSDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1076739 ACJA3T_RS14600 WP_059642617.1 3131871..3133376(+) (rpoN/rpoN1) [Burkholderia semiarida strain SYBS718]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCCCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCCGCGGACGGATCGCTGATCGCGCAGACGCCACCCGCATCGAACACCGAACCCGCCGCG
GCCAACGGCACCAGTACGTCCAGCGATCGCGCGGAACGCGACGAGCCGGCAGGCGCCGACGAATACGACGGCTATGCGTC
CGGCGACGGCAGCGACGGCCCGCAGTGGAATCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGATCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCCACGCTCGACGAAGTGCT
CGCCGATCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGTTGCTGCGTCTCGATCCGTCCCCCACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAC
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGTGCCGCTTACGGGAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGACCCGTCGGCCGGATCGTTGAAACAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTCGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGATAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691

Multiple sequence alignment