Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RGQ30_RS09305 Genome accession   NZ_AP028947
Coordinates   2028820..2030202 (-) Length   460 a.a.
NCBI ID   WP_130556181.1    Uniprot ID   A0AA86MIJ5
Organism   Limnobacter thiooxidans strain CS-K2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2023820..2035202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGQ30_RS09295 (RGQ30_18360) - 2024574..2027648 (-) 3075 WP_130556183.1 efflux RND transporter permease subunit -
  RGQ30_RS09300 (RGQ30_18370) - 2027645..2028745 (-) 1101 WP_130556182.1 efflux RND transporter periplasmic adaptor subunit -
  RGQ30_RS09305 (RGQ30_18380) radA 2028820..2030202 (-) 1383 WP_130556181.1 DNA repair protein RadA Machinery gene
  RGQ30_RS09310 (RGQ30_18390) alr 2030224..2031330 (-) 1107 WP_130556180.1 alanine racemase -
  RGQ30_RS09315 (RGQ30_18400) lplT 2031488..2032765 (+) 1278 WP_130556179.1 lysophospholipid transporter LplT -
  RGQ30_RS09320 (RGQ30_18410) - 2032814..2033515 (-) 702 WP_130556178.1 uracil-DNA glycosylase -
  RGQ30_RS09325 (RGQ30_18420) rimI 2033512..2033973 (-) 462 WP_130556177.1 ribosomal protein S18-alanine N-acetyltransferase -
  RGQ30_RS09330 (RGQ30_18430) tsaB 2033960..2034664 (-) 705 WP_130556176.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  RGQ30_RS09335 (RGQ30_18440) ispF 2034664..2035158 (-) 495 WP_130556175.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49276.88 Da        Isoelectric Point: 7.3644

>NTDB_id=107487 RGQ30_RS09305 WP_130556181.1 2028820..2030202(-) (radA) [Limnobacter thiooxidans strain CS-K2]
MAKSKSQYQCTECGSVFPKWQGQCTDCNSWNTLVESAVTATAPPKPSRFAALAAQSNTVIKLADIQAQDHPRFSSGVPEF
DRVLGGGMVPGMVVLLGGDPGIGKSTLLLQSMVTLAKTTSALYVSGEESAGQIALRAQRLGLEHANLSVLTEIQLEQIVA
QLEILKPQVLVIDSIQTLYTSELSAAPGSVSQVRECASQLTRLAKALGITTILVGHVTKEGTLAGPRVLEHIVDTVLYFE
GDSQSSFRLVRAFKNRFGAVNELGVFAMTDRGLKGVNNPSALFLSQQAEWVAGSCVMCTQEGARPLLVEIQALVDASHSP
APRRLSVGLEQNRLAMLLAVMHRHAGLQFFDQDIFINAVGGVRISEPAADLAIVLAMVSSLRNKRLPKELVVFGEIGLSG
ELRPAPRGQERLREAAKLGFTHAIVPASNAPKQPIDGIEVIGVDRVEKALAALRDKLDCF

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=107487 RGQ30_RS09305 WP_130556181.1 2028820..2030202(-) (radA) [Limnobacter thiooxidans strain CS-K2]
TTGGCCAAAAGTAAAAGTCAATATCAATGCACCGAGTGTGGTTCCGTATTCCCCAAATGGCAGGGGCAATGTACCGATTG
CAACAGCTGGAACACACTGGTTGAATCGGCTGTAACGGCCACTGCACCACCTAAGCCCAGCCGGTTTGCTGCACTTGCCG
CGCAAAGCAACACCGTCATCAAGCTTGCTGATATTCAGGCTCAGGACCACCCGCGATTCAGTAGCGGTGTGCCGGAGTTT
GATCGCGTGTTGGGTGGGGGCATGGTACCCGGCATGGTGGTGTTGCTGGGTGGCGATCCTGGTATTGGTAAATCAACCTT
GCTGCTGCAGTCCATGGTCACCTTGGCCAAAACCACCTCGGCCCTGTATGTCAGCGGTGAAGAGTCTGCAGGTCAAATTG
CATTGCGCGCGCAACGATTGGGTCTGGAGCACGCCAACCTCAGTGTGTTAACGGAAATACAACTGGAGCAGATCGTTGCT
CAGCTTGAAATACTGAAACCCCAGGTCCTGGTGATCGATTCGATCCAGACCCTTTACACCAGTGAATTAAGCGCAGCCCC
AGGTTCCGTCAGTCAGGTTCGCGAGTGTGCAAGCCAGTTGACCCGCCTGGCCAAAGCCTTGGGAATCACCACCATTCTGG
TCGGCCATGTCACCAAAGAAGGTACTTTGGCTGGCCCCCGTGTTCTCGAACACATTGTGGACACCGTCTTGTATTTTGAG
GGCGACTCGCAGTCGTCTTTCCGCCTGGTTCGAGCATTCAAGAACCGTTTTGGCGCCGTAAATGAACTGGGTGTCTTTGC
CATGACAGACCGGGGTTTGAAAGGGGTCAACAATCCGTCCGCCTTGTTTTTGTCTCAACAGGCTGAATGGGTTGCGGGCT
CCTGTGTCATGTGTACGCAGGAAGGGGCACGACCTTTGTTGGTCGAAATTCAGGCCCTGGTCGACGCGTCCCATTCGCCT
GCACCCCGGCGTTTGTCAGTGGGGCTTGAACAAAACCGCCTGGCCATGTTGCTGGCCGTGATGCATCGGCATGCGGGTTT
GCAGTTCTTTGACCAGGACATTTTCATCAATGCGGTGGGCGGGGTTCGAATTTCGGAACCTGCGGCTGACTTGGCGATTG
TGTTGGCCATGGTGTCCTCCCTGCGGAACAAGCGTTTGCCCAAAGAGCTGGTGGTTTTTGGTGAAATTGGCTTGTCAGGT
GAGTTGCGTCCTGCACCCCGCGGGCAGGAACGTTTGAGGGAAGCGGCCAAGTTGGGTTTTACCCATGCCATCGTTCCGGC
CAGCAATGCACCCAAGCAGCCCATCGACGGCATTGAAGTCATCGGTGTGGATCGGGTTGAGAAAGCGCTTGCCGCACTGA
GAGACAAGCTGGATTGTTTTTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(344-429)

AAA domain (AAA_25)

(82-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.366

99.783

0.483
  radA Streptococcus pneumoniae Rx1

45.714

98.913

0.452
  radA Streptococcus pneumoniae D39

45.714

98.913

0.452
  radA Streptococcus pneumoniae R6

45.714

98.913

0.452
  radA Streptococcus pneumoniae TIGR4

45.714

98.913

0.452
  radA Streptococcus mitis SK321

45.898

98.043

0.45
  radA Streptococcus mitis NCTC 12261

45.676

98.043

0.448

Multiple sequence alignment