Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACI65L_RS05640 Genome accession   NZ_CP174195
Coordinates   1244994..1246376 (-) Length   460 a.a.
NCBI ID   WP_010296280.1    Uniprot ID   -
Organism   Pectobacterium carotovorum strain PCC27     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1239994..1251376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI65L_RS05610 (ACI65L_001118) pepP 1240172..1241497 (-) 1326 WP_039507384.1 Xaa-Pro aminopeptidase -
  ACI65L_RS05615 (ACI65L_001119) - 1241572..1242159 (-) 588 WP_201879071.1 YecA family protein -
  ACI65L_RS05620 (ACI65L_001120) zapA 1242352..1242681 (+) 330 WP_010284667.1 cell division protein ZapA -
  ACI65L_RS05630 (ACI65L_001122) - 1242979..1243626 (+) 648 WP_201879072.1 5-formyltetrahydrofolate cyclo-ligase -
  ACI65L_RS05635 (ACI65L_001123) nadR 1243623..1244876 (-) 1254 WP_161508762.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  ACI65L_RS05640 (ACI65L_001124) radA 1244994..1246376 (-) 1383 WP_010296280.1 DNA repair protein RadA Machinery gene
  ACI65L_RS05645 (ACI65L_001125) serB 1246394..1247371 (-) 978 WP_119880570.1 phosphoserine phosphatase -
  ACI65L_RS05650 (ACI65L_001126) - 1247528..1248226 (+) 699 WP_201879073.1 YtjB family periplasmic protein -
  ACI65L_RS05655 (ACI65L_001127) - 1248281..1249207 (-) 927 WP_336846619.1 hypothetical protein -
  ACI65L_RS05660 (ACI65L_001128) prfC 1249508..1251097 (+) 1590 WP_039546948.1 peptide chain release factor 3 -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49618.21 Da        Isoelectric Point: 7.4218

>NTDB_id=1074819 ACI65L_RS05640 WP_010296280.1 1244994..1246376(-) (radA) [Pectobacterium carotovorum strain PCC27]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASVSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEITSGSSVMVVWEGTRPLLVEIQALVDQ
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKVPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=1074819 ACI65L_RS05640 WP_010296280.1 1244994..1246376(-) (radA) [Pectobacterium carotovorum strain PCC27]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGACTACCCGCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAATACCATTACCGAAGTCCGTCTGGCCTCGGCGTCTGTGTCACGTTCTGACCGTCTCACTGGCTATG
CAGGCGAAAGCGCTGGCGTCAGCCGGGTACAGAAGCTCTCGGAAATCAGTCTTGAAGCGCTGCCTCGCTTTTCTACCGGC
TTTCAGGAGTTTGACCGCGTTCTGGGCGGCGGTGTCGTTCCGGGCAGTGCCATTCTGATCGGCGGTAACCCCGGTGCAGG
TAAAAGTACCCTGCTGCTGCAAACGCTCTGCAAGCTGTCAGAGAACATGAAAACCCTGTACGTCACCGGTGAAGAATCAC
TACAGCAGGTGGCAATGCGGGCACATCGTCTCAATCTGCCAACGCAGAATCTCAATATGCTGTCAGAAACCAGCATCGAA
CAGATTTGCCTGATCGCCGAGCAGGAACAGCCGAAACTGATGGTGATCGACTCCATTCAGGTCATGCATCTCGCCGATAT
TCAATCGTCTCCCGGCAGCGTGGCGCAGGTGCGTGAAACCGCCGCCTACCTGACGCGCTTCGCCAAAACGCGCGGCGTTG
CTATCGTCATGGTCGGCCACGTCACCAAAGACGGTTCGCTCGCCGGGCCGAAAGTCTTGGAACACTGCATCGACTGCTCC
GTGCTGCTGGATGGCGATGCCGATTCCCGCTTCCGCACGCTGCGCAGCCATAAAAACCGTTTCGGCGCCGTTAACGAGTT
GGGCGTGTTCGCGATGACGGAACAAGGTCTACGCGAGATCAGCAATCCGTCAGCGATTTTCCTCAGCCGCGGGGACGAAA
TCACGTCCGGCAGTTCGGTCATGGTGGTGTGGGAAGGCACGCGTCCGCTGCTGGTCGAAATTCAGGCGCTGGTAGATCAA
TCGATGATGGCCAACCCGCGTCGCGTTGCGGTCGGGCTGGAGCAAAACCGATTAGCCATCCTGCTGGCGGTGCTGCATCG
ACACGGCGGCTTGCAGATGTCGGATCAGGATGTGTTCGTGAATGTCGTCGGCGGAGTGAAAGTCACCGAAACCAGTGCCG
ATCTGGCGCTACTGCTATCGCTGGTCTCCAGCTTCCGCGACCGCCCGCTGCCGCAAGATCTGGTCATCTTCGGTGAAGTC
GGTCTGGCGGGCGAAATCCGCCCGGTCCCCAGCGGACAAGAGCGGATTACCGAAGCCGCCAAACACGGTTTCAAACGTGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAGTCCCTGCCAGCATGCAGGTGTTCGGCGTGAAAAAGCTGGCCGATGCGC
TGGCGATCCTCGACGATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.261

100

0.483
  radA Streptococcus pneumoniae Rx1

42.982

99.13

0.426
  radA Streptococcus pneumoniae D39

42.982

99.13

0.426
  radA Streptococcus pneumoniae R6

42.982

99.13

0.426
  radA Streptococcus pneumoniae TIGR4

42.982

99.13

0.426
  radA Streptococcus mitis NCTC 12261

42.982

99.13

0.426
  radA Streptococcus mitis SK321

42.982

99.13

0.426

Multiple sequence alignment