Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RGQ30_RS01625 Genome accession   NZ_AP028947
Coordinates   364026..365546 (-) Length   506 a.a.
NCBI ID   WP_130558638.1    Uniprot ID   A0AA86IXE5
Organism   Limnobacter thiooxidans strain CS-K2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359026..370546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGQ30_RS01595 (RGQ30_03240) mutM 359284..360102 (+) 819 WP_130558642.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  RGQ30_RS01600 (RGQ30_03250) mutY 360099..361268 (+) 1170 WP_338284656.1 A/G-specific adenine glycosylase -
  RGQ30_RS01605 (RGQ30_03260) rapZ 361224..362093 (-) 870 WP_338284657.1 RNase adapter RapZ -
  RGQ30_RS01610 (RGQ30_03270) hprK 362086..363069 (-) 984 WP_130558640.1 HPr(Ser) kinase/phosphatase -
  RGQ30_RS01615 (RGQ30_03280) - 363074..363544 (-) 471 WP_040512070.1 PTS sugar transporter subunit IIA -
  RGQ30_RS01620 (RGQ30_03290) hpf 363655..363984 (-) 330 WP_130558639.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RGQ30_RS01625 (RGQ30_03300) rpoN/rpoN1 364026..365546 (-) 1521 WP_130558638.1 RNA polymerase factor sigma-54 Machinery gene
  RGQ30_RS01630 (RGQ30_03310) lptB 365559..366338 (-) 780 WP_420915152.1 LPS export ABC transporter ATP-binding protein -
  RGQ30_RS01635 (RGQ30_03320) lptA 366335..366883 (-) 549 WP_130558637.1 lipopolysaccharide transport periplasmic protein LptA -
  RGQ30_RS01640 (RGQ30_03330) lptC 366880..367467 (-) 588 WP_298219359.1 LPS export ABC transporter periplasmic protein LptC -
  RGQ30_RS01645 (RGQ30_03340) - 367464..368003 (-) 540 WP_130558635.1 KdsC family phosphatase -
  RGQ30_RS01650 (RGQ30_03350) - 368003..368995 (-) 993 WP_130558634.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 56888.18 Da        Isoelectric Point: 4.5420

>NTDB_id=107477 RGQ30_RS01625 WP_130558638.1 364026..365546(-) (rpoN/rpoN1) [Limnobacter thiooxidans strain CS-K2]
MKQSLQLKVSQNLTLTPQLQQSIRLLQLSTLELQQEIQQALAENPLLELADEEAMEGERTLSLDSSNGELHEPKGDSFEA
EPDPRKAPEPVSDQQAEQDNQGEEIVQRETIDSIEDNWEGDWRDNSGANADGDEEKPGLQLAAPDSSLHQHLIDQLRLTQ
ASERDKNLVLWLIDYLSDDGYLTVELEDLWETLQDEPDLEFEELSAALSLLQSFDPPGVGARNASECLLLQLAQRSDITC
QPSLLGNCQAIIRDHLGLLAQRDFTKLKKALNVDEDVLKVCHQIIRSLEPFPGARFRRIDDDHVVPDVIVKKLSGQWHVQ
LNPDVMPKIRINEMYASILRSHRGQSAQLSAQLQEARWLLKNVQQRFDTILRVSQAIVERQIGYFNHGEIAMQPLVLREI
ADLLGLHESTVSRVTTQKYMLTPYGLVELKYFFGSHVSTDSGGQASSTAIRALIKQLISAEDAKQPISDSQIAELLGQQG
IVVARRTVAKYRESLKIPPVNMRKSL

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=107477 RGQ30_RS01625 WP_130558638.1 364026..365546(-) (rpoN/rpoN1) [Limnobacter thiooxidans strain CS-K2]
ATGAAACAATCACTTCAGCTTAAAGTTTCCCAAAACCTCACGCTAACCCCTCAGCTACAGCAGTCCATTCGCCTGTTACA
ACTGTCCACGCTTGAATTGCAGCAGGAAATCCAGCAGGCCCTTGCCGAAAACCCGCTGCTAGAACTGGCAGATGAAGAAG
CGATGGAAGGCGAACGCACACTGAGCCTGGACAGCAGCAATGGTGAACTGCACGAACCCAAAGGCGACAGCTTTGAGGCT
GAACCCGACCCCCGTAAAGCGCCTGAACCCGTCAGCGACCAACAGGCTGAACAGGACAACCAGGGCGAAGAAATTGTTCA
GCGTGAAACCATTGACTCGATTGAGGACAATTGGGAAGGCGATTGGCGCGACAACAGCGGCGCCAACGCCGACGGCGATG
AGGAAAAGCCAGGCCTGCAGCTTGCTGCGCCTGACAGCAGCCTGCACCAACATCTGATCGACCAGTTGCGCCTGACGCAA
GCCAGTGAACGGGACAAAAACCTCGTGCTGTGGCTGATCGACTACCTGAGTGACGACGGCTACCTCACCGTTGAATTGGA
AGACCTTTGGGAAACCCTGCAAGACGAACCCGACCTGGAGTTTGAAGAACTCAGCGCGGCCCTGAGCCTGCTGCAAAGCT
TTGACCCACCTGGTGTGGGCGCACGCAATGCTTCCGAATGCCTGCTGTTGCAACTGGCCCAAAGGTCCGACATCACCTGC
CAGCCCTCACTACTCGGCAACTGCCAGGCCATTATCCGCGACCACCTTGGACTGCTCGCCCAACGCGACTTCACCAAGTT
GAAAAAAGCACTCAACGTGGACGAAGACGTGCTGAAGGTTTGCCACCAGATCATTCGTTCACTTGAACCATTTCCTGGCG
CGCGCTTTCGCCGAATTGACGACGACCATGTAGTTCCCGACGTAATTGTCAAGAAACTTAGCGGCCAATGGCATGTGCAA
TTGAACCCGGATGTCATGCCAAAAATCCGAATCAATGAAATGTATGCCTCTATTTTGCGCTCTCATCGAGGGCAAAGTGC
GCAACTCAGCGCCCAACTTCAAGAGGCCCGCTGGCTATTAAAAAACGTTCAGCAGCGATTTGACACTATTTTACGAGTCA
GCCAGGCCATCGTTGAAAGGCAGATTGGCTACTTCAACCATGGTGAAATTGCCATGCAGCCCTTGGTTTTGCGAGAAATT
GCAGACCTTCTCGGTCTTCATGAATCGACTGTGTCAAGGGTTACCACGCAAAAATATATGTTGACGCCCTATGGGTTGGT
TGAGCTAAAGTATTTCTTCGGGAGTCATGTTTCCACCGACTCGGGTGGGCAGGCTTCCAGCACGGCCATCCGTGCGCTCA
TCAAGCAACTGATTTCTGCGGAGGACGCCAAGCAGCCGATATCTGACAGCCAAATCGCCGAACTACTCGGTCAACAGGGC
ATCGTGGTCGCAAGACGCACGGTCGCTAAATACCGGGAATCACTGAAAATACCCCCGGTGAACATGCGAAAGTCGCTTTA
A

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(349-504)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-335)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.926

100

0.585

Multiple sequence alignment